Cutting Edge: Role of B Lymphocytes in Protective Immunity AgainstSalmonella typhimuriumInfection

Abstract

Infection of mice with Salmonella typhimurium gives rise to a disease similar to human typhoid fever caused by S. typhi. Since S. typhimurium is a facultative intracellular bacterium, the requirement of B cells in the immune response against S. typhimurium is a longstanding matter of debate. By infecting mice on a susceptible background and deficient in B cells (Igμ−/− mice) with different strains of S. typhimurium, we could for the first time formally clarify the role of B cells in the response against S. typhimurium. Compared with Igμ+/+ mice, LD50 values in Igμ−/− mice were reduced during primary, and particularly secondary, oral infection with virulent S. typhimurium. After systemic infection, Igμ−/− mice cleared attenuated aroA− S. typhimurium, but vaccine-induced protection against systemic infection with virulent S. typhimurium involved both B cell-dependent and -independent effector mechanisms. Thus, B cell-mediated immunity plays a distinct role in control of S. typhimurium in susceptible mice.

Delivery of the Immunosuppressive Antigen Salp15 to Antigen-Presenting Cells bySalmonella entericaSerovar TyphimuriumaroAMutants

“…This finding correlates with increased splenomegaly in these mice, resulting from a prominent infiltration of CD11b ϩ cells (data not shown). Our results agree with those of previous studies indicating increased mutant Salmonella burdens in mice that are unable to mount T-cell responses (17).…”

Intranasal HIV-1-gp160-DNA/gp41 Peptide Prime-Boost Immunization Regimen in Mice Results in Long-Term HIV-1 Neutralizing Humoral Mucosal and Systemic Immunity

“…They proliferate immediately in the presence of Ags and differentiate into plasma B cells that will produce specific Abs. B cells can also present Ag, stimulating T cell and cytokine responses (57)(58)(59). To evaluate the specific immune responses induced by our immunogens, we performed a number of immunizations with rgp160/Rev DNA/ELDKWAS peptide, with and without the CCR5 peptide.…”

Identification of genetic loci controlling bacterial clearance in experimental Salmonella enteritidis infection: an unexpected role of Nramp1 (Slc11a1) in the persistence of infection in mice

“…Ses2 explained 6% of the genetic variance and Ses3, 4% (Table 2). In these cases also, the C57BL/6J allele is protective and contributes in a recess- 33,9,36,25 D7Mit76,83,62,31,323,38,237,44,189 D8Mit3,41,56 D9Mit218,25,306,37 D10Mit80,38,42,10,205 D11Mit77,20,5,4,124,61 D12Mit182,49,12,59,46,60,14,Nds2 D13Mit236,139,147,30,36,151 D14Mit11,52,30 D15Mit12,26,46,47,29,42,242,159,35 D16Mit81,4,47,…”

“…Mice carrying a null allele at CD28 are susceptible to infection with S. typhimurium and die late during the course of infection. 31 The humoral response is also involved in the clearance of Salmonella as shown by studies performed with the mouse mutant Btk (Bruton's tyrosine kinase or xid for X-linked immunodeficiency). These mice present a defect in peripheral B-cell activation that results in deficient antibody production and the inability to eliminate Salmonella infection.…”