Cutting Edge: CD1a+ Antigen-Presenting Cells in Human Dermis Respond Rapidly to CCR7 Ligands

Angel, Catherine E.; George, Emad; Brooks, Anna; Ostrovsky, Lena; Brown, Tim; Dunbar, P. Rod

doi:10.4049/jimmunol.176.10.5730

Cited by 95 publications

(134 citation statements)

References 19 publications

(10 reference statements)

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“…Dermal dendritic cells are a heterogeneous cell population, both in humans (Nestle et al 1993;Angel et al 2006;Angel et al 2007) and mice (Bursch et al 2007;Ginhoux et al 2007;Poulin et al 2007;Shklovskaya et al 2008). In contrast to LCs, DDCs display an amoeboid morphology (Ng et al 2008a), more characteristic of a migratory cell (Gunzer et al 2000).…”

Section: Dendritic Cells In the Skinmentioning

confidence: 99%

Visualizing dendritic cell migration within the skin

Roediger

Smith

et al. 2008

Histochem Cell Biol

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Dendritic cells (DCs) within the skin are a heterogeneous population of cells, including Langerhans cells of the epidermis and at least three subsets of dermal DCs. Collectively, these DCs play important roles in the initiation of adaptive immune responses following antigen challenge of the skin as well as being mediators of tolerance to self-antigen. A key functional aspect of cutaneous DCs is their migration both within the skin and into lymphatic vessels, resulting in their emigration to draining lymph nodes. Here, we discuss our current understanding of the requirements for successful DC migration in and from the skin, and introduce some of the microscopic techniques developed in our laboratory to facilitate a better understanding of this process. In particular, we detail our current use of multi-photon excitation (MPE) microscopy of murine skin to dissect the migratory behavior of DCs in vivo.

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Section: Dendritic Cells In the Skinmentioning

confidence: 99%

Visualizing dendritic cell migration within the skin

Roediger

Smith

et al. 2008

Histochem Cell Biol

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“…Although we could detect MGL ϩ DC in the emigrated population, their numbers were slightly lower than expected. This can be explained by the DC maturation that accompanies the emigration from skin explants (20). DC maturation abrogates MGL expression and thus reduces the final numbers of DCs that are still MGL positive after completing the emigration from skin (8).…”

Section: Mgl Mediates Retention Of Immature Dcsmentioning

confidence: 99%

The C-Type Lectin Macrophage Galactose-Type Lectin Impedes Migration of Immature APCs

Vliet

Paessens

Berg

et al. 2008

The Journal of Immunology

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Dendritic cells (DCs) are the most potent APCs of the immune system that seed the peripheral tissues and lymphoid organs. In an immature state, DCs sample their surroundings for incoming pathogens. Upon Ag encounter, DCs mature and migrate to the lymph node to induce adaptive immune responses. The C-type macrophage galactose-type lectin (MGL), expressed in immature DCs, mediates binding to glycoproteins carrying GalNAc moieties. In the present study, we demonstrate that MGL ligands are present on the sinusoidal and lymphatic endothelium of lymph node and thymus, respectively. MGL binding strongly correlated with the expression of the preferred MGL ligand, α-GalNAc-containing glycan structures, as visualized by staining with the α-GalNAc-specific snail lectin Helix pomatia agglutinin. MGL+ cells were localized in close proximity of the endothelial structures that express the MGL ligand. Strikingly, instead of inducing migration, MGL mediated retention of human immature DCs, as blockade of MGL interactions enhanced DC trafficking and migration. Thus, MGL+ DCs are hampered in their migratory responses and only upon maturation, when MGL expression is abolished; these DCs will be released from their MGL-mediated restraints.

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“…[2][3][4] In murine models, the precise role of Langerhans cells remains a matter of debate [4][5][6][7][8][9][10] ; in contrast, APCs that have migrated from the murine dermis have been shown to be essential for the initiation of certain T-lymphocyte responses. 2,11 We recently identified a human dermal APC subset capable of migrating in response to lymph node-homing chemokines and stimulating naive CD4 T lymphocytes 12 ; furthermore, a recent study has shown that they also stimulate CD8 T lymphocytes. 13 Given the pivotal role of dermal APCs for the generation of immune responses in mice, we sought to confirm that their putative human counterparts migrate to lymph nodes draining human skin.…”

Section: Introductionmentioning

confidence: 99%