Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts

Hé, Zhiguo; Forest, Fabien; Bernard, Adeline; Gauthier, Anne‐Sophie; Montard, R.; Péoc’h, Michel; Jumelle, Clotilde; Courrier, Emilie; Perrache, Chantal; Gain, Philippe; Thuret, Gilles

doi:10.1167/iovs.16-20256

Cited by 37 publications

(45 citation statements)

References 44 publications

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“…[192a,208] He et al decellularized cornea lamellae obtained by femtosecond laser using different methods and found 1% SDS with DNAse to be optimal. [208] The lamellae were then recellularized with an endothelial cell line that adhered and formed a tight endothelium expressing Na + /K + ATPase and tight junctions. While the reports are promising, the Descemet's membrane was not present.…”

Section: Decellularized Corneamentioning

confidence: 99%

Designing Scaffolds for Corneal Regeneration

Ahearne

Fernández‐Pérez

Masterton

et al. 2020

Adv Funct Materials

111

106

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Corneal blindness is one of the most common causes of vision loss worldwide, affecting millions of people. To treat these patients, researchers have been examining different approaches to engineer corneal scaffolds suitable for transplantation. Scaffolds have been developed to replace part or all of the cornea depending on the patient requirements. Both acellular and cell-seeded scaffolds have been tested in animal models. Materials that have been under investigation for manufacturing scaffolds include collagen, silk fibroin, amniotic membrane, decellularized cornea, fibrin, chitosan, gelatin, agarose, alginate, and hyaluronic acid in addition to several synthetic polymers. Different combinations of materials, fiber crosslinking techniques, and incorporation of bioactive molecules have also been examined. Factors such as the physical properties, cytocompatibility, degradation behavior, and optical characteristics have to be considered when selecting a suitable scaffold material. Recent advancements in materials fabrication techniques such as bioprinting, electrospinning, and different collagen alignment techniques, allow scaffolds to be generated that more accurately mimic the structure of the corneal stroma. A number of scaffolds have commenced clinical trials to determine their suitability for corneal regeneration.

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Section: Decellularized Corneamentioning

confidence: 99%

Designing Scaffolds for Corneal Regeneration

Ahearne

Fernández‐Pérez

Masterton

et al. 2020

Adv Funct Materials

111

106

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“…To overcome difficulties concerning biocompatibility and immunoreaction of the host when using engineered tissue, one promising approach is the use of decellularized tissue. Decellularized corneal tissues as a scaffold came into the focus of recent research as this fulfills most of the biological, mechanical, and immunogenic requirements (He et al, 2016;Yam et al, 2016). As a potential source for decellularized tissue substitutes, human cadaveric donor corneas unsuitable for transplantation due to low endothelial cell density or other quality reasons have been used .…”

mentioning

confidence: 99%

Decellularized human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction

Mertsch

Hasenzahl

Reichl

et al. 2020

J Tissue Eng Regen Med

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The shortage of donor corneas as well as the limitations of tissue substitutes leads to the necessity to develop alternative materials for ocular surface reconstruction. Corneal surface substitutes must fulfill specific requirements such as high transparency, low immunogenicity, and mechanical stability combined with elasticity. This in vitro study evaluates a decellularized matrix secreted from human corneal fibroblasts (HCF) as an alternative material for ocular surface reconstruction. HCF from human donors were cultivated with the supplementation of vitamin C to form a stable and thick matrix. Furthermore, due to enhanced cultivation time, a three‐dimensional like multilayered construct which partly mimics the complex structure of the corneal stroma could be generated. The formed human cell‐based matrices (so‐called cell sheets [CS]) were subsequently decellularized. The complete cell removal, collagen content, ultrastructure, and cell toxicity of the decellularized CS (DCS) as well as biomechanical properties were analyzed. Surgical feasibility was tested on enucleated porcine eyes. After decellularization and sterilization, a transparent, thick, cell free, and sterile tissue substitute resulted, which allowed expansion of limbal epithelial stem cells with no signs of cytotoxicity, and good surgical feasibility. DCS seem to be a promising new corneal tissue substitute derived from human cells without the limitation of donor material; however, future in vivo studies are necessary to further elucidate its potential for ocular surface reconstruction.

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“…Although decellularized and layered human corneal lamellae have shown biocompatibility with human CECs, making endothelialized human lamellae is a feasible option for endothelial keratoplasty in the future. Currently, re‐endothelialization by human CECs is only possible in vitro . This is a limitation of decellularized corneas that cannot be directly used in many corneal diseases with endothelial cell damage.…”

Section: Recellularizationmentioning

confidence: 99%

“…Currently, re-endothelialization by human CECs is only possible in vitro. 57,58 This is a limitation of decellularized corneas that cannot be directly used in many corneal diseases with endothelial cell damage.…”

Section: Recellul Ariz Ationmentioning

confidence: 99%

Decellularization methods for developing porcine corneal xenografts and future perspectives

Isidan

Liu

et al. 2019

Xenotransplantation

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Corneal transplantation is the only option to cure corneal opacities. However, there is an imbalance between supply and demand of corneal tissues in the world. To solve the problem of corneal shortage, corneal xenotransplantation studies have been implemented in the past years using porcine corneas. The corneal xenografts could come from (a) wild‐type pigs, (b) genetically engineered pigs, (c) decellularized porcine corneas, and (d) decellularized porcine corneas that are recellularized with human corneal cells, eventually with patients' own cells, as in all type of xenografts. All approaches except, the former would reduce or mitigate recipient immune responses. Although several techniques in decellularization have been reported, there is still no standardized protocol for the complete decellularization of corneal tissue. Herein, we reviewed different decellularization methods for porcine corneas based on the mechanism of action, decellularization efficacy, biocompatibility, and the undesirable effects on corneal ultrastructure. We compared 9 decellularization methods including: (a) sodium dodecyl sulfate, (b) triton x‐100, (c) hypertonic saline, (d) human serum with electrophoresis, (e) high hydrostatic pressure, (f) freeze‐thaw, (h) nitrogen gas, (h) phospholipase A2, and (i) glycerol with chemical crosslinking methods. It appears that combined methods could be more useful to perform efficient corneal decellularization.

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Cutting and Decellularization of Multiple Corneal Stromal Lamellae for the Bioengineering of Endothelial Grafts

Abstract: This study demonstrates that thin human corneal lamellae could have high potential as carriers in next-generation therapy for endothelial dysfunctions.

Cited by 37 publications

References 44 publications

Designing Scaffolds for Corneal Regeneration

Designing Scaffolds for Corneal Regeneration

Decellularized human corneal stromal cell sheet as a novel matrix for ocular surface reconstruction

Decellularization methods for developing porcine corneal xenografts and future perspectives

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