Cutinase and pectinase in cotton bioscouring: an innovative and fast bioscouring process

Agrawal, Piyush; Nierstrasz, Vincent; Bouwhuis, G.H.; Warmoeskerken, Marinus

doi:10.1080/10242420802332558

Cited by 42 publications

(26 citation statements)

References 24 publications

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“…Earlier reports showed that the fungal cutinase from Fusarium solani pisi has potential use for cotton cuticle degradation and exhibits a good synergistic effect with pectinase, an enzyme utilized to degrade pectin, in the scouring of cotton fiber (1,7,8,14). Moreover, site-directed mutagenesis has been performed to replace the specific amino acid residues near the active site of cutinase (3) to improve its hydrolytic activity toward polyesters.…”

mentioning

confidence: 99%

Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Their Potential Application in Bioscouring

Zhang

Chen

et al. 2010

Appl Environ Microbiol

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Cutinase from Thermobifida fusca is thermally stable and has potential application in the bioscouring of cotton in the textile industry. In the present study, the carbohydrate-binding modules (CBMs) from T. fusca cellulase Cel6A (CBM Cel6A ) and Cellulomonas fimi cellulase CenA (CBM CenA ) were fused, separately, to the carboxyl terminus of T. fusca cutinase. Both fusion enzymes, cutinase-CBM Cel6A and cutinase-CBM CenA , were expressed in Escherichia coli and purified to homogeneity. Enzyme characterization showed that both displayed similar catalytic properties and pH stabilities in response to T. fusca cutinase. In addition, both fusion proteins displayed an activity half-life of 53 h at their optimal temperature of 50°C. Compared to T. fusca cutinase, in the absence of pectinase, the binding activity on cotton fiber was enhanced by 2% for cutinase-CBM Cel6A and by 28% for cutinase-CBM CenA , whereas in the presence of pectinase, the binding activity was enhanced by 40% for the former and 45% for the latter. Notably, a dramatic increase of up to 3-fold was observed in the amount of released fatty acids from cotton fiber by both cutinase-CBM fusion proteins when acting in concert with pectinase. This is the first report of improving the scouring efficiency of cutinase by fusing it with CBM. The improvement in activity and the strong synergistic effect between the fusion proteins and pectinase suggest that they may have better applications in textile bioscouring than the native cutinase.Cotton fiber has a multilayered structure, with its outermost surface being the cuticle that is cross-linked to the primary cell wall of cotton fiber by esterified pectin substances. The major component of the cuticle is cutin, an insoluble polyester composed mainly of saturated C 16 and C 18 hydroxy and epoxy fatty acids (14,16,27,38). During the process of scouring in the textile industry, the cuticle layer has to be removed in order to improve the wettability of cotton fiber, which then facilitates uniform dyeing and finishing. Traditionally, this process is performed by hot hydrolysis in alkaline medium, which not only consumes large quantities of water and energy but also causes severe pollution and fiber damage (20,21,33). Therefore, environment-friendly scouring methods based on biocatalysts have been actively sought (2,30,36).Cutinase is a multifunctional esterase capable of degrading the cutin component of the cuticle. Earlier reports showed that the fungal cutinase from Fusarium solani pisi has potential use for cotton cuticle degradation and exhibits a good synergistic effect with pectinase, an enzyme utilized to degrade pectin, in the scouring of cotton fiber (1,7,8,14). Moreover, site-directed mutagenesis has been performed to replace the specific amino acid residues near the active site of cutinase (3) to improve its hydrolytic activity toward polyesters. More recently, a cutinase from the thermophilic bacterium Thermobifida fusca has been identified and overexpressed in Escherichia coli in our laboratory (10). The...

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confidence: 99%

Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Their Potential Application in Bioscouring

Zhang

Chen

et al. 2010

Appl Environ Microbiol

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“…Over the years, biotechnology has been employed in the textile fi bre processing such as fl ax and kenaf retting , ramie fi bre extraction (Zheng et al 2001;Basu et al 2011;Basu et al 2009), cotton bio-scouring (Agrawal et al 2008;Rajendran et al 2011), silk degumming (Arami et al 2007), wool functionalization (Queiroga et al 2007;Hossain et al 2009), etc.…”

Section: Biotechnological Fi Bre Extraction In Bamboomentioning

confidence: 98%

Bio-processing of bamboo fibres for textile applications: a mini review

Gao

et al. 2012

Biocatalysis and Biotransformation

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This review addresses the potential biotechnological techniques for textile fi bre extraction (degumming), mainly focusing on bamboo, one of the main and recently developed textile natural fi bres in China. The latest developments in microbial and enzymatic processing for ecological fi bre extraction as well as the development of a molecular technique for natural fi bre bio-treatment are highlighted.

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“…The necessary information and knowledge about the low-temperature wax removal, pectin removal, efficient wetting and use of chelators is available now (Agrawal et al 2007(Agrawal et al , 2008. The optimum conditions for enzymatic scouring are 50 mM Tris -HCl buffer, pH 8 -9, at 30 ° C, 1 g Triton X-100 L -1 , 2 g Baysolex L -1 as chelator and enzyme concentration of 100 U g-fabric -1 and 52 U g-fabric -1 for cutinase and pectinase, respectively.…”

Section: Ultrasound-assisted Benign One-step Cotton Scouringmentioning

confidence: 99%

“…The diffusion-based slow scouring process can be converted to a faster process by effi cient wetting and by intensifying mass transport. Effi cient wetting in cotton scouring is possible with non-ionic surfactants (Lenting et al 2002;Agrawal et al 2008). To intensify the mass transport in a textile processes, techniques such as the use of vacuum technology (Lenting & Warmoeskerken 2004) and ultrasound are being investigated.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-boosted enzymatic cotton scouring process with cutinase and pectate lyase

Agrawal

Nierstrasz

Warmoeskerken

2010

Biocatalysis and Biotransformation

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The synergistic effect between power ultrasound and enzymes in an enzymatic scouring process has been studied. The scouring enzymes were Fusarium solani pisi cutinase (EC 3.1.1.74) and pectate lyase (EC 4.2.2.2). In different stages of the scouring process, power ultrasound with a pre-optimized power of 0.57 W cm(-2) and a frequency of 30 kHz was applied. It was found that ultrasound shortens the enzymatic scouring process time dramatically; less than 5 min was required to achieve the desired scouring expressed in terms of hydrophilicity of the cotton fiber. The results obtained have been explained in terms of mass transfer intensification by ultrasound (so-called 'sono-mechanics') and its effect on the enzyme kinetics (so-called 'sono-chemistry'). This latter effect has been found by applying ultrasound in a homogeneous enzymatic reaction in which mass transfer did not play any role. The kinetics of product formation in a homogeneous system was carried out using poly-D-galacturonic acid as a model substrate

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Cutinase and pectinase in cotton bioscouring: an innovative and fast bioscouring process

Cited by 42 publications

References 24 publications

Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Their Potential Application in Bioscouring

Characterization of Thermobifida fusca Cutinase-Carbohydrate-Binding Module Fusion Proteins and Their Potential Application in Bioscouring

Bio-processing of bamboo fibres for textile applications: a mini review

Ultrasound-boosted enzymatic cotton scouring process with cutinase and pectate lyase

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