Cutinase—A new tool for biomodification of synthetic fibers

Silva, Carla; Carneiro, F.; O’Neill, Alexandre; Fonseca, Luís P.; Cabral, Joaquim M. S.; Guebitz, Georg M.; Cavaco‐Paulo, Artur

doi:10.1002/pola.20684

Cited by 116 publications

(99 citation statements)

References 8 publications

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“…The latter was also reported to be active in the hydrolysis of poly(lactic acid) as well as cutinases from Thermobifida halotolerans [70,77]. Finally, cutinase from Fusarium solani is currently reported as the only cutinase able to hydrolyze Polyamide 6,6 [80].…”

Section: Cutinases As Biocatalysts For Polymers Hydrolysismentioning

confidence: 99%

“…During the last 10 years, with the aim to expand the biocatalytical tools for polymer hydrolysis and modification, several cutinases have been tested for their ability to hydrolyze polyesters (Table 2). Fusarium solani [80] The scientific work was focused on the hydrolysis of the most relevant industrial polymers, in particular poly(ethylene terephthalate) (PET). Cutinases from various Thermobifida species demonstrated a good selectivity on PET [72,73].…”

Section: Cutinases As Biocatalysts For Polymers Hydrolysismentioning

confidence: 99%

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Nature Inspired Solutions for Polymers: Will Cutinase Enzymes Make Polyesters and Polyamides Greener?

et al. 2016

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Abstract:The polymer and plastic sectors are under the urge of mitigating their environmental impact. The need for novel and more benign catalysts for polyester synthesis or targeted functionalization led, in recent years, to an increasing interest towards cutinases due to their natural ability to hydrolyze ester bonds in cutin, a natural polymer. In this review, the most recent advances in the synthesis and hydrolysis of various classes of polyesters and polyamides are discussed with a critical focus on the actual perspectives of applying enzymatic technologies for practical industrial purposes. More specifically, cutinase enzymes are compared to lipases and, in particular, to lipase B from Candida antarctica, the biocatalyst most widely employed in polymer chemistry so far. Computational and bioinformatics studies suggest that the natural role of cutinases in attacking natural polymers confer some essential features for processing also synthetic polyesters and polyamides.

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Section: Cutinases As Biocatalysts For Polymers Hydrolysismentioning

confidence: 99%

Section: Cutinases As Biocatalysts For Polymers Hydrolysismentioning

confidence: 99%

Nature Inspired Solutions for Polymers: Will Cutinase Enzymes Make Polyesters and Polyamides Greener?

et al. 2016

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“…48 A produção de fibras de poliéster é maior que a de fibras de algodão. Devido à importância das fibras sintéticas, existe um grande interesse em pesquisa relacionada à melhora dos processos de produção, bem como à melhora das características do produto.…”

Section: Tratamento E Lavagem De Tecidosunclassified

“…Silva et al 48 utilizaram a cutinase de Fusarium solani pisi para modificar a superfície de fibras sintéticas de poliéster, poliamida e acrílicos. A cutinase foi escolhida para atuar na modificação das fibras devido a sua hidrofobicidade e ação em biopoliésteres presentes na cutina das plantas.…”

Section: Tratamento E Lavagem De Tecidosunclassified

Cutinases fúngicas: propriedades e aplicações industriais

et al. 2008

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Recebido em 18/12/07; aceito em 20/6/08; publicado na web em 10/11/08 FUNGAL CUTINASES: PROPERTIES AND INDUSTRIAL APPLICATIONS -REVIEW.Cutinases (EC 3.1.1.74) are also known as cutin hidrolases. These enzymes share catalytic properties of lipases and esterases, presenting a unique feature of being active regardless the presence of an oil-water interface, making them interesting as biocatalysts in several industrial processes involving hydrolysis, esterification and trans-esterification reactions. They are also active in different reaction media, allowing their applications in different areas such as food industry, cosmetics, fine chemicals, pesticide and insecticide degradation, treatment and laundry of fiber textiles and polymer chemistry. The present review describes the characteristics, potential applications and new perspectives for these enzymes.Keywords: cutinase; industrial applications; biochemical characteristics. introduçÃoCutinases são enzimas que catalisam a hidrólise de um biopoliéster insolúvel, a cutina, componente estrutural da cutícula das plantas.1 Este poliéster é composto do seguintes ácidos graxos: ω-hidróxi ácidos graxos, ácido diidroxidróxi palmítico, e ácidos 18-hidróxi-9,10,-epóxi C 18 saturados e Δ12 insaturados, e ácidos 9,10,18-triidróxi C 18 saturados e Δ12 monoinsaturados. A composição da cutina é dependente da espécie, porém, em geral, em plantas de crescimento rápido predominam os ácidos de 16 carbonos, particularmente diidroxipalmitato, enquanto em plantas de crescimento lento uma mistura de ácidos de 16 e 18 carbonos é encontrada. A cutinase libera hidroliticamente todos os tipos de monômeros a partir do polímero. 2Além da hidrólise, assim como as lipases, as cutinases também são capazes de catalisar reações reversas, como esterificação, transesterificação (interesterificação, alcoólise e acidólise) e aminólise, sendo que a atividade de água do meio reacional é um dos fatores determinantes para cada classe de reação. 3,4 Segundo Brenda 5 a cutinase é classificada como EC 3.1.1.3 com nome recomendado de triacilglicerol lipase, e nome sistemático de triacilglicerolacil-hidrolase. Também é classificada, pela mesma fonte, como 3.1.l.74 com nome recomendado de cutinase e nome sistemático de cutina hidrolase, ou seja, uma esterase que hidrolisa a cutina, podendo agir e ser classificada também como lipase.As cutinases apresentam diversas características interessantes para aplicações em uma ampla gama de produtos, variando de detergentes a alimentos.6 Conseqüentemente, tem sido desenvolvida intensa pesquisa abordando suas estruturas, funções, purificação e aplicações, visando o desenvolvimento de novos processos empregando este grupo de enzimas. 1O presente artigo descreve as características e potenciais aplicações das cutinases fúngicas em processos industriais. Características das cutinasesCutinases são dos menores membros da família serina hidrolase, que apresenta a tríade catalítica clássica composta por serina, histidina e um grupo carboxil. A sub-família das cutinases consiste em ...

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“…Synthetic polyesters (PET), most commonly used polymer in beverage containers, food packaging and electronic industry [42], represents a total of 50% of the global market for textile fibers [43]. An increased hydrophilicity of the fiber is required to facilitate the dyeing process of these fibers using enzymatic treatments as opposed to using harsh chemicals.…”

Section: Conflict Of Interestsmentioning

confidence: 99%

Expression and characterization of a cutinase (AnCUT2) from Aspergillus niger

et al. 2016

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Cutin hydrolase (EC 3.1.1.74), an extracellular polyesterase found in pollens, bacteria and fungi, is an efficient catalyst that exhibits hydrolytic activity on a variety of water-soluble esters, synthetic fibers, plastics and triglycerides. Thus, cutinase can be used in various applications such as ester synthesis, bio-scouring, food and detergent industries. Ancut2 is one of five genes encoding cutinases present in the Aspergillus niger ATCC 10574 genome. The cDNA of Ancut2 comprising of an open reading frame of 816 bp encoding a protein of 271 amino acid residues, was isolated and expressed in Pichia pastoris. The partially purified recombinant cutinase exhibited a molecular mass of approximately 40 kDa. The enzyme showed highest activity at 40°C with a preference for acidic pH (5.0-6.0). AnCUT2 showed hydrolytic activity towards various p-nitrophenyl esters with preference towards shorter chain esters such as p-nitrophenyl butyrate (C4). Scanning Electron Microscopy demonstrated that AnCUT2 was capable of modifying surfaces of synthetic polycaprolactone and polyethylene terephthalate plastics. The properties of this enzyme suggest that it may be applied in synthetic fiber modification and fruit processing industries.

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Cutinase—A new tool for biomodification of synthetic fibers

Cited by 116 publications

References 8 publications

Nature Inspired Solutions for Polymers: Will Cutinase Enzymes Make Polyesters and Polyamides Greener?

Nature Inspired Solutions for Polymers: Will Cutinase Enzymes Make Polyesters and Polyamides Greener?

Cutinases fúngicas: propriedades e aplicações industriais

Expression and characterization of a cutinase (AnCUT2) from Aspergillus niger

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