Cuticular hydrocarbons of triatomines

Juárez, M. Patricia; Fernández, G. Calderón

doi:10.1016/j.cbpa.2006.08.031

Cited by 64 publications

(49 citation statements)

References 106 publications

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“…gambiae CHCs represent the majority (>80%) of the total mosquito HCs, with a composition similar to that of the internal pool. In insects such as triatomines and cockroaches, only ∼10-20% of the HCs are transported to the surface, and the majority of the methyl-branched HCs are retained in the hemolymph as reservoir (27,28).…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae

Balabanidou

Kampouraki

MacLean

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

322

370

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The role of cuticle changes in insecticide resistance in the major malaria vector Anopheles gambiae was assessed. The rate of internalization of 14 C deltamethrin was significantly slower in a resistant strain than in a susceptible strain. Topical application of an acetone insecticide formulation to circumvent lipid-based uptake barriers decreased the resistance ratio by ∼50%. Cuticle analysis by electron microscopy and characterization of lipid extracts indicated that resistant mosquitoes had a thicker epicuticular layer and a significant increase in cuticular hydrocarbon (CHC) content (∼29%). However, the CHC profile and relative distribution were similar in resistant and susceptible insects. The cellular localization and in vitro activity of two P450 enzymes, CYP4G16 and CYP4G17, whose genes are frequently overexpressed in resistant Anopheles mosquitoes, were analyzed. These enzymes are potential orthologs of the CYP4G1/2 enzymes that catalyze the final step of CHC biosynthesis in Drosophila and Musca domestica, respectively. Immunostaining indicated that both CYP4G16 and CYP4G17 are highly abundant in oenocytes, the insect cell type thought to secrete hydrocarbons. However, an intriguing difference was indicated; CYP4G17 occurs throughout the cell, as expected for a microsomal P450, but CYP4G16 localizes to the periphery of the cell and lies on the cytoplasmic side of the cell membrane, a unique position for a P450 enzyme. CYP4G16 and CYP4G17 were functionally expressed in insect cells. CYP4G16 produced hydrocarbons from a C18 aldehyde substrate and thus has bona fide decarbonylase activity similar to that of dmCYP4G1/2. The data support the hypothesis that the coevolution of multiple mechanisms, including cuticular barriers, has occurred in highly pyrethroid-resistant An. gambiae. malaria | insecticide resistance | hydrocarbons | mosquito cuticle | cytochrome P450

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Section: Discussionmentioning

confidence: 99%

Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae

Balabanidou

Kampouraki

MacLean

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

322

370

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“…We found that fatty acyl coA elongase transcripts were more abundant in mature oocyte tissue under diapause inducing SD conditions relative to diapause averting LD conditions in replicate temperate (diapausing) but not tropical (non-diapausing) populations (figure 1). Because fatty acyl coA elongases encode proteins involved in the synthesis of very long chain surface lipids that are known to mediate desiccation resistance in a diverse group of insects (Blomquist et al 1987;Vaz et al 1988;Juárez 1994Juárez , 2004Yoder et al 1995;Benoit & Denlinger 2007;Juárez & Fernández 2007), we hypothesized that the increased transcript abundance of this gene might be related to the previously documented increased survival of diapause relative to non-diapause eggs under desiccating conditions (Sota & Mogi 1992). Consistent with this hypothesis, we found that in a temperate population, diapause eggs had approximately 30 per cent more surface lipids (more than 99% hydrocarbon) than nondiapause eggs, but that eggs from a tropical population reared under SD and LD conditions did not differ in surface lipid quantities (figure 2).…”

Section: Discussionmentioning

confidence: 99%

The molecular physiology of increased egg desiccation resistance during diapause in the invasive mosquito,Aedes albopictus

et al. 2010

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Photoperiodic diapause is a crucial adaptation to seasonal environmental variation in a wide range of arthropods, but relatively little is known regarding the molecular basis of this important trait. In temperate populations of the mosquito Aedes albopictus, exposure to short-day (SD) lengths causes the female to produce diapause eggs. Tropical populations do not undergo a photoperiodic diapause. We identified a fatty acyl coA elongase transcript that is more abundant under SD versus long-day (LD) photoperiods in mature oocyte tissue of replicate temperate, but not tropical, A. albopictus populations. Fatty acyl CoA elongases are involved in the synthesis of long chain fatty acids (hydrocarbon precursors). Diapause eggs from a temperate population had one-third more surface hydrocarbons and one-half the water loss rates of non-diapause eggs. Eggs from a tropical population reared under SD and LD photoperiods did not differ in surface hydrocarbon abundance or water loss rates. In both a temperate and tropical population, composition of hydrocarbon chain lengths did not differ between eggs from SD versus LD conditions. These results implicate the expression of fatty acyl coA elongase and changes in quantity, but not composition, of egg surface hydrocarbons as important components of increased desiccation resistance during diapause in A. albopictus.

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“…The cuticular surface is covered by a thin layer of lipids (mainly hydrocarbons, wax esters, and fatty alcohols) and with free or esterified fatty acids. These lipids play a major role in preventing lethal desiccation by altering the absorption of chemicals and microorganism penetration, and they also participate in chemical communication events [72][73][74]. Some fatty acids, by being part of the epicuticular waxes in T. infestans, have a strong action of regrouping (e.g., hexacosanoic acid) [75].…”

Section: Triatominae Bugs (Vector Of Chagas Disease)mentioning

confidence: 99%

Terpenes: Natural Products for Controlling Insects of Importance to Human Health—A Structure-Activity Relationship Study

Dambolena

Zunino

Herrera

et al. 2016

Psyche: A Journal of Entomology

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Many insects affect food production and human health, and in an attempt to control these insects the use of synthetic insecticides has become widespread. However, this has resulted in the development of resistance in these organisms, human diseases, contamination of food, and pollution of the environment. Plants natural products and essential oil components such as terpenes and phenylpropenes have been shown to have a significant potential for insect control. However, the molecular properties related to their insecticidal activity are not well understood. The purpose of this review is to provide an overview of the toxicity of terpene compounds against three insects of importance to human health: lice, cockroaches, and Triatominae bugs and to evaluate which molecular descriptors are important in the bioactivity of terpenes. For the insects studied, quantitative structure-activity relationship (QSAR) studies were performed in order to predict the insecticidal activity of terpene compounds. The obtained QSAR models indicated that the activity of these compounds depends on their ability to reach the targets and to interact with them. The QSAR analysis can be used to predict the bioactivities of other structurally related molecules. Our findings may provide an important contribution in the search for new compounds with insecticidal activity.

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Cuticular hydrocarbons of triatomines

Cited by 64 publications

References 106 publications

Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae

Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae

The molecular physiology of increased egg desiccation resistance during diapause in the invasive mosquito,Aedes albopictus

Terpenes: Natural Products for Controlling Insects of Importance to Human Health—A Structure-Activity Relationship Study

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