Cutibacterium modestum sp. nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense

Dekio, Itaru; Sakamoto, Mitsuo; Suzuki, Tomo; Yuki, Masahiro; Kinoshita, Shigeru; Murakami, Yoshiyuki; Ohkuma, Moriya

doi:10.1099/ijsem.0.004058

Cited by 22 publications

(20 citation statements)

References 19 publications

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“…Five of these 11 isolates were identified as C. acnes despite considerable differences (97.5–98.2%) towards the 16S rRNA gene sequence of the C. acnes type strain (ATCC 6919 T ). We also detected within the 16S rRNA gene sequence of C. modestum M12 T (LC466959) three mismatches compared to the whole-genome-sequence of M12 T (BJEN01000000): position 3 a G instead of A, position 11 a T instead of C, and position 1484 an A instead of G (Dekio et al 2020 ) (see also Table 1 ).…”

Section: Resultsmentioning

confidence: 89%

“…Retrospective analysis of 16S rRNA gene sequences in public databases resulted in 11 entries with highly similar (99.2–100%) 16S rRNA gene sequences since 2001 (excluding C. modestum M12 T ). The corresponding isolates originated from different countries and sources showing that this organism has a clinical relevance (Table 1 ), (Dekio et al 2020 ; Kunishima et al 2001 ; Lin et al 2010 ). Five of these 11 isolates were identified as C. acnes despite considerable differences (97.5–98.2%) towards the 16S rRNA gene sequence of the C. acnes type strain (ATCC 6919 T ).…”

Section: Resultsmentioning

confidence: 99%

“…Genome sequences of six additional “ P. humerusii ” strains are present in public databases (Table 2 ), (Butler-Wu et al 2011 ; Dekio et al 2020 ). Table 2 also includes genome sequence data of our isolate 602588-20-USB, C. modestum strain M12 T , and C. acnes ATCC 6919 T for comparison.…”

Section: Resultsmentioning

confidence: 99%

“…Very recently, a Japanese group described an isolate from a patient with inflamed meibomian glands for which they formally proposed the name Cutibacterium modestum sp. nov. (Dekio et al 2020 ).…”

Section: Introductionmentioning

confidence: 98%

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Cutibacterium modestum and “Propionibacterium humerusii” represent the same species that is commonly misidentified as Cutibacterium acnes

Goldenberger

Søgaard

Cuénod

et al. 2021

Antonie van Leeuwenhoek

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Cutibacterium spp. play an increasing role in soft tissue and implant-associated infections. We isolated a novel Cutibacterium spp. from an implant and investigated this isolate using multiple identification approaches. Correct identification was hampered by inconsistent reference data. The isolate was characterised using conventional methods such as Gram stain, MALDI-TOF MS, and antimicrobial susceptibility testing against multiple antimicrobials. Partial 16S rRNA gene sequencing and whole genome sequencing were also performed. In addition, we summarised the available published sequence data and compared prior data to our strain. Conventional phenotypic identification of our isolate resulted in Cutibacterium spp. After analysis of 16S rRNA gene and genome sequences, our isolate was identified as C. modestum, a very recently described species. The 16S rRNA gene analysis was hampered by three incorrect nucleotides within the 16S rRNA gene reference sequence of C. modestum M12T (accession no. LC466959). We also clearly demonstrate that this novel species is identical to tentatively named “Propionibacterium humerusii”. Retrospective data analysis indicates that C. modestum is a clinically important Cutibacterium species often misidentified as C. acnes. The isolation and identification of Cutibacterium spp. is still a challenge. The correct description of very recently named C. modestum and the availability of a correct 16S rRNA sequence of the type strain may help to clarify the taxonomical uncertainty concerning “P. humerusii”. The novel C. modestum is an additional, clinically important species within the genus Cutibacterium and may represent a new member of the human skin microbiome.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Cutibacterium modestum and “Propionibacterium humerusii” represent the same species that is commonly misidentified as Cutibacterium acnes

Goldenberger

Søgaard

Cuénod

et al. 2021

Antonie van Leeuwenhoek

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“…These findings likely reflect the greater reliance of proteolytic activity as a source of carbon and energy among commensal propionibacteria given their nutrient-scarce microenvironment [ 10 ]. Although representative species for all four genera that compose the former Propionibacterium genus were considered in this analysis, the analysis needs to be expanded further to encompass newly available sequenced species [ 91 , 92 , 93 ] before it can be generalized more broadly. Only P. acnes was detected as auxotrophic for some amino acids.…”

Section: Resultsmentioning

confidence: 99%

A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity

McCubbin

Gonzalez-Garcia

Palfreyman

et al. 2020

Genes

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Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequence data, the accurate annotation of the available genomes, and the functional characterization of genes. The recent completion of the genome sequences for several species has led researchers to reassess the taxonomical classification of the genus Propionibacterium, which has been divided into several new genres. Such data also enable a comparative genomic approach to annotation and provide a new opportunity to revisit our understanding of their metabolism. Using pan-genome analysis combined with the reconstruction of the first high-quality Propionibacterium genome-scale metabolic model and a pan-metabolic model of current and former members of the genus Propionibacterium, we demonstrate that despite sharing unique metabolic traits, these organisms have an unexpected diversity in central carbon metabolism and a hidden layer of metabolic complexity. This combined approach gave us new insights into the evolution of Propionibacterium metabolism and led us to propose a novel, putative ferredoxin-linked energy conservation strategy. The pan-genomic approach highlighted key differences in Propionibacterium metabolism that reflect adaptation to their environment. Results were mathematically captured in genome-scale metabolic reconstructions that can be used to further explore metabolism using metabolic modeling techniques. Overall, the data provide a platform to explore Propionibacterium metabolism and a tool for the rational design of strains.

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Meibomian Gland Dysfunction Clinical Practice Guidelines

Amano¹,

Shimazaki

Yokoi

et al. 2023

Jpn J Ophthalmol

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Cutibacterium modestum sp. nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense

Cited by 22 publications

References 19 publications

Cutibacterium modestum and “Propionibacterium humerusii” represent the same species that is commonly misidentified as Cutibacterium acnes

Cutibacterium modestum and “Propionibacterium humerusii” represent the same species that is commonly misidentified as Cutibacterium acnes

A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity

Meibomian Gland Dysfunction Clinical Practice Guidelines

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