Cutaneous Wound Healing: A Review about Innate Immune Response and Current Therapeutic Applications

Adib, Yara; Bensussan, Armand; Michel, Laurence

doi:10.1155/2022/5344085

Cited by 39 publications

(28 citation statements)

References 175 publications

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“…After 60 days, the material underwent a disintegration process compatible with a hydrolytic degradation that produced the separation of the apatite particles from the network of collagen fibers where they were immersed (Figure 12D,E). Large blood vessels were observed in all the periods evaluated, which play a critical role in the tissue repair process and increase significantly in the proliferative phase when necessary to guarantee adequate blood flow [56]. Figure 12F shows a large blood vessel in the implantation area at 90 days with fragments of collagen material and bovine apatite.…”

Section: Results Of Subdermal Implantation Of F2 (Inteross Collagen)mentioning

confidence: 97%

“…12D,E). Large blood vessels were observed in all the periods evaluated, which play a critical role in the tissue repair process and increase significantly in the proliferative phase when necessary to guarantee adequate blood flow [56]. Figure 12F shows a large blood vessel in the implantation area at 90 days with fragments of collagen material and bovine apatite.…”

Section: Results Of Subdermal Implantation Of F2 (Inteross Collagen)mentioning

confidence: 97%

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Biocompatibility Assessment of Two Commercial Bone Xenografts by In Vitro and In Vivo Methods

2022

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Bone substitutes based on xenografts have been used for a long time in bone regeneration thanks to their inductive capacity for bone tissue regeneration. Some bone-based scaffolds have been modified by adding collagen and other proteins to improve their regenerative capacity and prevent migration and aggregation, especially particles. However, rejection of this graft has been reported due to protein residues caused by poor material preparation. We compared the in vitro and in vivo biological response of two commercial xenografts (InterOss®, F1 and InterOss® Collagen, F2) and a commercial porcine collagen membrane (InterCollagen® Guide, F3) as a rapid degradation control. Fourier Transform Infrared Spectroscopy (FT-IR) analysis evidenced the presence of hydroxyl, orthophosphate, and carbonate groups of the xenografts and amide groups of collagen. Thermogravimetric analysis (TGA) of the xenografts demonstrated their thermal stability and the presence of a few amounts of organic material. The study by differential scanning calorimetry showed the presence of endothermic peaks typical of the dehydration of the xenografts (F1 and F2) and for the collagen membrane (F3), the beginning of structural three-dimensional protein changes. Subsequently, in vitro biocompatibility tests were carried out for the materials with Artemia salina and MTT cell viability with HeLa cells, demonstrating the high biocompatibility of the materials. Finally, in vivo biocompatibility was studied by implanting xenografts in biomodels (Wistar rats) at different periods (30, 60, and 90 days). The F1 xenograft (InterOss) remained remarkably stable throughout the experiment (90 days). F2 (InterOss Collagen) presented a separation of its apatite and collagen components at 60 days and advanced resorption at 90 days of implantation. Finally, the collagen membrane (F3) presented faster resorption since, at 90 days, only some tiny fragments of the material were evident. All the in vivo and in vitro test results demonstrated the biocompatibility of the xenografts, demonstrating the potential of these materials for tissue engineering.

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Section: Results Of Subdermal Implantation Of F2 (Inteross Collagen)mentioning

confidence: 97%

Section: Results Of Subdermal Implantation Of F2 (Inteross Collagen)mentioning

confidence: 97%

Biocompatibility Assessment of Two Commercial Bone Xenografts by In Vitro and In Vivo Methods

2022

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“…A capsule surrounding the implanted material is a normal finding during biomaterial studies in animal models. During the healing process, the acute inflammation caused by the surgical lesion occurs with the implanted material [ 44 ]. Once the acute phase is overcome, the remaining material produces a chronic inflammatory phase that finishes once the situation is resolved [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Synthesis, Characterization, and Optimization Studies of Polycaprolactone/Polylactic Acid/Titanium Dioxide Nanoparticle/Orange Essential Oil Membranes for Biomedical Applications

et al. 2022

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The development of scaffolds for cell regeneration has increased because they must have adequate biocompatibility and mechanical properties to be applied in tissue engineering. In this sense, incorporating nanofillers or essential oils has allowed new architectures to promote cell proliferation and regeneration of new tissue. With this goal, we prepared four membranes based on polylactic acid (PLA), polycaprolactone (PCL), titanium dioxide nanoparticles (TiO2-NPs), and orange essential oil (OEO) by the drop-casting method. The preparation of TiO2-NPs followed the sol–gel process with spherical morphology and an average size of 13.39 nm ± 2.28 nm. The results show how the TiO2-NP properties predominate over the crystallization processes, reflected in the decreasing crystallinity percentage from 5.2% to 0.6% in the membranes. On the other hand, when OEO and TiO2-NPs are introduced into a membrane, they act synergistically due to the inclusion of highly conjugated thermostable molecules and the thermal properties of TiO2-NPs. Finally, incorporating OEO and TiO2-NPs promotes tissue regeneration due to the decrease in inflammatory infiltrate and the appearance of connective tissue. These results demonstrate the great potential for biomedical applications of the membranes prepared.

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“…iNKT cells are a unique lineage of T lymphocytes that regulate both innate and adaptive immunity. Undoubtedly, innate and adaptive immunity play essential roles in wound healing [ 55 , 56 ]. Bai et al have generated macrophage-specific MED1/apolipoprotein E (ApoE) double-deficient (MED1 ΔMac /ApoE −/− ) mice and found that MED1 ablation decreased the binding of peroxisome proliferator-activated receptor γ (PPARγ), which promoted the polarization of macrophages from M2 to M1 and enhanced innate immune stimulation, thereby increasing atherosclerosis [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

MED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway

Meng

Guo

et al. 2022

IJMS

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Mediator complex subunit 1 (MED1) is a coactivator of multiple transcription factors and plays a key role in regulating epidermal homeostasis as well as skin wound healing. It is unknown, however, whether it plays a role in healing oral mucosal wounds. In this study, we investigate MED1’s functional effects on oral mucosal wound healing and its underlying mechanism. The epithelial-specific MED1 null (Med1epi−/−) mice were established using the Cre-loxP system with C57/BL6 background. A 3 mm diameter wound was made in the cheek mucosa of the 8-week-old mice. In vivo experiments were conducted using HE staining and immunostaining with Ki67 and uPAR antibodies. The in vitro study used lentiviral transduction, scratch assays, qRT-PCR, and Western blotting to reveal the underlying mechanisms. The results showed that ablation of MED1 accelerated oral mucosal wound healing in 8-week-old mice. As a result of ablation of MED1, Activin A/Follistatin expression was altered, resulting in an activation of the JNK/c-Jun pathway. Similarly, knockdown of MED1 enhanced the proliferation and migration of keratinocytes in vitro, promoting re-epithelialization, which accelerates the healing of oral mucosal wounds. Our study reveals a novel role for MED1 in oral keratinocytes, providing a new molecular therapeutic target for accelerated wound healing.

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Cutaneous Wound Healing: A Review about Innate Immune Response and Current Therapeutic Applications

Cited by 39 publications

References 175 publications

Biocompatibility Assessment of Two Commercial Bone Xenografts by In Vitro and In Vivo Methods

Biocompatibility Assessment of Two Commercial Bone Xenografts by In Vitro and In Vivo Methods

Synthesis, Characterization, and Optimization Studies of Polycaprolactone/Polylactic Acid/Titanium Dioxide Nanoparticle/Orange Essential Oil Membranes for Biomedical Applications

MED1 Ablation Promotes Oral Mucosal Wound Healing via JNK Signaling Pathway

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