Cutaneous mucosal immune-parameters and intestinal immune-relevant genes expression in streptococcal-infected rainbow trout (Oncorhynchus mykiss): A comparative study with the administration of florfenicol and olive leaf extract

Shiry, Nima; Khoshnoodifar, Khadije; Alavinia, Seyed Jalil

doi:10.1016/j.fsi.2020.10.023

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…The direct and indirect effects of A. bisporus derivatives have been investigated in various studies (Muszynska et al, 2015b Hoseinifar et al (2016) showed that the highest relative expression of tumor necrosis factor-alpha (TNF-α) and Interleukin 1 beta (IL-1β) genes in the skin of carps received 1% WBMP in the diet. Similar results were observed in the mentioned parameters in Oncorhynchus mykiss (Harikrishnan et al, 2018;Shiry et al, 2020). Enrichment of the cat sh (Clarias gariepinus) diet with powder of A. bisporus enhanced weight gain, regulated blood cells ratio, and induced phagocytic and lysozyme activities (Safari et al, 2018).…”

Section: Introductionsupporting

confidence: 74%

“…Cytokine molecules can stimulate immune responses (Gruss, 1996). Gene expression of this in ammatory cytokine has also been induced following administration of antibiotics similar to the dose mentioned in rainbow trout challenged with infectious agents (Shiry et al, 2020). Compatible with these results, Khodadadian Zou et al (2016) reported that white button mushroom powder (1 and 2% in the diet) had a signi cant effect on increasing the expression of the IL-1β gene in the intestines of common carp.…”

Section: Discussionmentioning

confidence: 96%

“…The tubes were immediately moved to a nitrogen tank. Samples from this part of the trial were also kept in the freezer at -80° C until use for RNA extraction (Shiry et al, 2020).…”

Section: Samplingmentioning

confidence: 99%

“…The primers of the mentioned genes with a housekeeping gene (beta-actin) used in the tests were introduced in Table 3. These sequences were designed by Primer3 online software, then BioNeer corporation (Daejeon, South Korea) made them (Shiry et al, 2020). The real-time PCR detection system was an iCycler iQ™ (Bio-RAD Laboratories, USA), and the procedure of relative expression measuring was conducted according to the ΔΔCt method using the IQ5 software (Bio-RAD).…”

Section: Measurement Of Immune-related Genes Expressionmentioning

confidence: 99%

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Skin mucosal immune-parameters and expression of the immune-relevant genes in Danio rerio treated by white button mushroom (Agaricus bisporus)

Ezatrahimi

Soltanian

Hoseinifar

2023

Preprint

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This study evaluates using different levels of the white button mushroom powder (WBMP) on some mucosal innate immune parameters (lysozyme, protease, esterase, alkaline phosphatase activities, and total immunoglobulin levels), and the relative expression of some principal immune-relevant genes (lysozyme, TNF-α, and IL-1β) in the zebra danio intestine. Zebrafish specimens (1.75 ± 0.25 g) were divided into experimental units based on the additives to a diet including 5, 10, and 20 g of WBMP per kg of food weight, alone or in conjunction with the antibiotic (10 mg/kg BW), and the AGRIMOS (1 g/kg food weight). Following the 11-day experimental duration, the skin mucus and intestine were sampled. To assess the immune gene expression, the real-time PCR detection system was conducted according to the ΔΔCt method using the IQ5 software (Bio-RAD). Results showed that all groups had a significant increase in terms of mucosal lysozyme activity compared to the control group. Examination of total immunoglobulin, protease, esterase, and ALP activity in fish under experimental treatment showed that there was no significant difference between the trial groups and the control groups. The most expression of the lysozyme gene was related to the group that was separately taken the lower concentration (5 g per kg of FW) of WBMP. In conclusion, the amount of 1% mushroom powder in the diet can improve its immune function. Our recommendation is that given the positive effects that mushroom powder added on the diet alone, avoid taking antibiotics for this purpose.

show abstract

Section: Introductionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 96%

“…The tubes were immediately moved to a nitrogen tank. Samples from this part of the trial were also kept in the freezer at -80° C until use for RNA extraction (Shiry et al, 2020).…”

Section: Samplingmentioning

confidence: 99%

Section: Measurement Of Immune-related Genes Expressionmentioning

confidence: 99%

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Skin mucosal immune-parameters and expression of the immune-relevant genes in Danio rerio treated by white button mushroom (Agaricus bisporus)

Ezatrahimi

Soltanian

Hoseinifar

2023

Preprint

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“…Previous studies have demonstrated that A. hydrophila induces intestinal inflammation in zebrafish and grass carp ( 33 , 34 ). Moreover, the IgM expression in the intestine of rainbow trout was significantly increased after infection with Streptococcus iniae ( 35 ). However, the immune response and function of IgM against A. hydrophila infection in the common carp intestine remains largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila

Dong

Kong

et al. 2022

Front. Immunol.

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Immunoglobulin (Ig) M is an important immune effector that protects organisms from a wide variety of pathogens. However, little is known about the immune response of gut mucosal IgM during bacterial invasion. Here, we generated polyclonal antibodies against common carp IgM and developed a model of carp infection with Aeromonas hydrophila via intraperitoneal injection. Our findings indicated that both innate and adaptive immune responses were effectively elicited after A. hydrophila infection. Upon bacterial infection, IgM+ B cells were strongly induced in the gut and head kidney, and bacteria-specific IgM responses were detected in high levels both in the gut mucus and serum. Moreover, our results suggested that IgM responses may vary in different infection strategies. Overall, our findings revealed that the infected common carp exhibited high resistance to this representative enteropathogenic bacterium upon reinfection, suggesting that IgM plays a key role in the defense mechanisms of the gut against bacterial invasion. Significantly, the second injection of A. hydrophila induces strong local mucosal immunity in the gut, which is essential for protection against intestinal pathogens, providing reasonable insights for vaccine preparation.

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The Antimicrobial Activities of Oleuropein and Hydroxytyrosol

Zorić

Kosalec

2022

Promising Antimicrobials From Natural Products

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Cutaneous mucosal immune-parameters and intestinal immune-relevant genes expression in streptococcal-infected rainbow trout (Oncorhynchus mykiss): A comparative study with the administration of florfenicol and olive leaf extract

Cited by 6 publications

References 35 publications

Skin mucosal immune-parameters and expression of the immune-relevant genes in Danio rerio treated by white button mushroom (Agaricus bisporus)

Skin mucosal immune-parameters and expression of the immune-relevant genes in Danio rerio treated by white button mushroom (Agaricus bisporus)

Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila

The Antimicrobial Activities of Oleuropein and Hydroxytyrosol

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