Cutaneous Lymphocyte Antigen Is Expressed on Memory/Effector B Cells in the Peripheral Blood and Monocytoid B Cells in the Lymphoid Tissues

Yoshino, Tadashi; Okano, Mitsuhiro; Chen, Hong Li; Tsuchiyama, Junjiro; Kondo, Eisaku; Nishiuchi, Ritsuo; Teramoto, Norihiro; Nishizaki, Kazunori; Akagi, Tadaatsu

doi:10.1006/cimm.1999.1552

Cited by 18 publications

(19 citation statements)

References 26 publications

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“…36,No. 2 mice sensitized with ovalbumin via the epicutaneous route were found to display a less-severe histological AD phenotype (assessed by skin thickening) compared to wild type (WT) mice.…”

Section: Reviewmentioning

confidence: 99%

“…36,No. 2 independent roles in the maintenance of skin immunity as well as in its defense against tumors and invading pathogens ( Figure 1).…”

Section: Reviewmentioning

confidence: 99%

“…CLA binds to E-selectin, which is upregulated on cutaneous vascular endothelium under inflammatory conditions [30,31] but is also constitutively expressed at low levels in normal uninflamed skin [32,33]. CLA is expressed on a population of human circulating, class-switched memory B cells [34][35][36]. B cells activated in vitro with phorbol myristate acetate (PMA) upregulated CLA expression and exhibited enhanced binding to E-selectin [34], indicating that appropriate stimulatory conditions can induce CLA expression in B cells, potentially enabling cutaneous migration.…”

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confidence: 99%

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Revisiting the role of B cells in skin immune surveillance

Egbuniwe

Karagiannis

Nestle

et al. 2015

Trends in Immunology

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Whereas our understanding of the skin immune system has increased exponentially in recent years, the role of B cells in cutaneous immunity remains poorly defined. Recent studies have revealed the presence of B cells within lymphocytic infiltrates in chronic inflammatory skin diseases and cutaneous malignancies including melanoma, and have examined their functional significance in these settings. We review these findings and discuss them in the context of the current understanding of the role of B cells in normal skin physiology, as well as in both animal and human models of skin pathology. We integrate these findings into a model of cutaneous immunity wherein crosstalk between B cells and other skin-resident immune cells plays a central role in skin immune homeostasis.

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Section: Reviewmentioning

confidence: 99%

“…36,No. 2 independent roles in the maintenance of skin immunity as well as in its defense against tumors and invading pathogens ( Figure 1).…”

Section: Reviewmentioning

confidence: 99%

mentioning

confidence: 99%

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Revisiting the role of B cells in skin immune surveillance

Egbuniwe

Karagiannis

Nestle

et al. 2015

Trends in Immunology

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“…All TBC used in our experiments expressed CD75, a marker for mature, mostly germinal center B cells, and CD78, a marker for mantle zone B cells [30]. Both the groups of Montoya et al [45]and Yoshino et al [47]seem thus to have isolated different populations of B cells from those used in our studies.…”

Section: Discussionmentioning

confidence: 99%

“…These cells did not adhere to P selectin. Yoshino et al [47]have reported HECA-452 reactivity with concomitant adhesive properties for E-selectin on peripheral blood memory-type immunoglobulin class switched B cells, but did not look for PSGL-1 expression. We ourselves have not yet succeeded in isolating sufficient numbers of memory-type B cells to repeat these experiments.…”

Section: Discussionmentioning

confidence: 99%

Tonsillar B Cells Do Not Express PSGL-1, but a Significant Fraction Displays the Cutaneous Lymphocyte Antigen and Exhibits Effective E- and P-Selectin Ligand Activity

Armerding

Fuhlbrigge²,

Kieffer³

et al. 2001

Int Arch Allergy Immunol

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Skin-homing T cells are defined by the expression of the cutaneous lymphocyte-associated antigen (CLA) which enables the cells to selectively bind to vascular endothelial E-selectin close to sites of cutaneous inflammation, an initial step in the effective extravasation from blood into the inflamed tissue. Essentially all CLA on T cells decorates the backbone of the P-selectin glycoprotein ligand-1 (PSGL-1). In this study we show that human peripheral blood B cells (PBBC) and tonsillar B cells (TBC) do not display PSGL-1 in fluorescence-activated cell sorter analysis using different murine monoclonal antibodies and polyclonal rabbit anti-PSGL-1 antiserum. A significant population of TBC, however, expresses a HECA-452-reactive epitope. These cells represent nonactivated IgM⁺/IgG^– mature B lymphocytes. Up to 50% of the TBC in a given preparation strongly bind to E- and up to 79% to P-selectin. The shear stress resistance in a parallel-plate flow chamber system was high. Neuraminidase treatment of TBC totally and O-sialoglycoprotein endopeptidase partially diminished HECA-452 reactivity and reduced E- but not P-selectin ligand activities. Mocarhagin had no effect in the assays. The data suggest a different ligand for P-selectin and a distinct glycoprotein carrier for the E-selectin ligand as compared to T cells or other leukocytes. Adhesion to P-selectin, however, still required sulfation of the ligand for function. Western blots of TBC cell lysates detected a >240-kD HECA-452-reactive material that was resistant to reducing conditions. Anti-PSGL-1 did not reveal immunoreactive material in these cell lysates. B cell activation did neither significantly change HECA positivity nor induce PSGL-1 expression. Cultured, activated TBC, however, maintained expression of the integrin α4β7. Human peripheral blood B cells had similar cell surface characteristics to TBC. Our observations suggest that several adhesion molecules may be involved in B cell homing which include CLA, the P-selectin ligand, and structures such as α4β7.

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Primary Cutaneous Diffuse Large B Cell Lymphoma Including the Leg Type and Precursor B Cell Lymphoblastic Lymphoma

2016

The Cutaneous Lymphoid Proliferations

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Cutaneous Lymphocyte Antigen Is Expressed on Memory/Effector B Cells in the Peripheral Blood and Monocytoid B Cells in the Lymphoid Tissues

Cited by 18 publications

References 26 publications

Revisiting the role of B cells in skin immune surveillance

Revisiting the role of B cells in skin immune surveillance

Tonsillar B Cells Do Not Express PSGL-1, but a Significant Fraction Displays the Cutaneous Lymphocyte Antigen and Exhibits Effective E- and P-Selectin Ligand Activity

Primary Cutaneous Diffuse Large B Cell Lymphoma Including the Leg Type and Precursor B Cell Lymphoblastic Lymphoma

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