Cutaneous abscess caused by Corynebacterium lactis in a companion dog

Antunes, João Marcelo Azevedo de Paula; Ribeiro, Márcio Garcia; Demoner, Larissa de Castro; Ramos, Juliana Nunes; Baio, Paulo Victor Pereira; Simpson-Louredo, Liliane; Santos, Cíntia Silva; Hirata, Raphael; Ferioli, Raquel Beneton; Romera, Adriana Resmond Cruz; Vieira, Verônica Viana; Mattos‐Guaraldi, Ana Luíza

doi:10.1016/j.vetmic.2015.04.014

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…C. lactis and other Corynebacterium species are frequently found in raw milk ( 14 , 31 ), and some isolates of Corynebacterium bovis and C. amycolatum are causative agents of mastitis in dairy cows ( 39 , 40 ). C. lactis was eventually linked to infections in companion animals, but it is not considered a pathogen so far and the determinants of infection remain unclear ( 41 , 42 ). Interestingly, a putative BGC similar to that of corynaridin exists in the genome of an isolate of C. striatum from an intensive care unit ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis

et al. 2023

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Bacteriocins are antimicrobial peptides produced by bacteria to fend off competitors in ecological niches and are considered to be important factors influencing the composition of microbial communities. However, bacteriocin production by bacteria of the genus Corynebacterium has been a hitherto neglected trait, although its species are ubiquitous in nature and make up large parts of the microbiome of humans and animals.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis

et al. 2023

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“…In dogs, several species have been isolated including Corynebacterium auricanis, Corynebacterium urealyticum, Corynebacterium mustelae, Corynebacterium ulcerans and Corynebacterium lactis. [6][7][8][9][10] None of these strains have been reported to be the causative agent of PPM or intramuscular abscesses, although C. lactis has been isolated from a percutaneous abscess in a dog. 9 Corynebacterium pseudotuberculosis in horses can present in different forms, with the most common being external abscess formation in the pectoral and ventral abdominal muscles.…”

Section: Discussionmentioning

confidence: 99%

“…[6][7][8][9][10] None of these strains have been reported to be the causative agent of PPM or intramuscular abscesses, although C. lactis has been isolated from a percutaneous abscess in a dog. 9 Corynebacterium pseudotuberculosis in horses can present in different forms, with the most common being external abscess formation in the pectoral and ventral abdominal muscles. 11 However, C. pseudotuberculosis has also been associated with deep intramuscular abscesses in horses.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5] In dogs, CSpp are considered transient bacterial flora and have been associated with dermatitis, otitis, endocarditis, urinary tract infections, bronchopneumonia and cutaneous abscesses. 3,[6][7][8][9][10] In horses, Corynebacterium pseudotuberculosis most commonly causes external abscesses in the pectoral and ventral abdominal region and ulcerative lymphangitis. 11,12 However, deep intramuscular abscesses have also been reported causing severe lameness.…”

Section: Introductionmentioning

confidence: 99%

“…CSpp have been reported to cause disease in different species, including humans, horses, dogs, cats, sheep and goats, and may have zoonotic potential 3–5 . In dogs, CSpp are considered transient bacterial flora and have been associated with dermatitis, otitis, endocarditis, urinary tract infections, bronchopneumonia and cutaneous abscesses 3,6–10 . In horses, Corynebacterium pseudotuberculosis most commonly causes external abscesses in the pectoral and ventral abdominal region and ulcerative lymphangitis 11,12 .…”

Section: Introductionmentioning

confidence: 99%

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Recurrent pyogranulomatous polymyositis and intramuscular abscesses secondary to Corynebacterium species in a young beagle

Mahon,

Albertini,

Marsh

et al. 2023

Vet Record Case Reports

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A 16‐month‐old, male, entire beagle presented for suspected relapse of steroid‐responsive meningitis‐arteritis 12 months after diagnosis. Following improvement with immunosuppressive treatment, the dog developed lethargy and right pelvic limb lameness. Neuroanatomical localisation was to the L4–S3 spinal cord segments/associated peripheral nerves. Magnetic resonance imaging revealed peripherally contrast‐enhancing, fluid‐isointense, cavitary lesions within the right and left great adductor, gracilis muscles and right semimembranosus, internal and external obturator, superficial glutaeal and coccygeal muscles. Culture and sensitivity of muscle fine‐needle aspiration revealed Corynebacterium species without antibiotic resistance. Clinical signs resolved on amoxicillin‐clavulanate (25 mg/kg/12 h) and clindamycin (20 mg/kg/12 h), but relapsed after 6 weeks. Addition of marbofloxacin (2 mg/kg/24 h) achieved clinical resolution. After 2 weeks, the dog relapsed and the owner elected euthanasia. Postmortem biopsies of the gracilis and semitendinosus muscles revealed pyogranulomatous polymyositis. Corynebacterium species should be considered a differential diagnosis for pyogranulomatous polymyositis in dogs. Lack of response to appropriate antibiotic treatment in immunosuppressed dogs may carry a poor prognosis.

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Identification, Characterization and Mode of Action of Corynaridin, a Novel Linaridin from Corynebacterium lactis

Pashou

Reich

Weixler

et al. 2022

Preprint

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Genome analysis of Corynebacterium lactis revealed a bacteriocin gene cluster encoding a putative bacteriocin of the linaridin family of ribosomally synthesized and posttranslationally modified peptides (RIPPs). The locus harbors typical linaridin modification enzymes but lacks genes for a decarboxylase and methyltransferase, which is unusual for type B linaridins. Supernatants of Corynebacterium lactis RW3-42 showed antimicrobial activity against Corynebacterium glutamicum. Deletion of the precursor gene crdA clearly linked the antimicrobial activity of the producer strain to the identified gene cluster. Following purification, we observed potent activity of the peptide against Actinobacteria, mainly other members of the genus Corynebacterium including the pathogenic species Corynebacterium striatum and Corynebacterium amycolatum. Also, low activity against some Firmicutes was observed, but no activity against Gram-negative species. The peptide is resilient towards heat but sensitive to proteolytic degradation by trypsin and proteinase K. Notably, time-kill kinetics and experiments using live biosensors to monitor membrane integrity suggest bactericidal activity that does not involve formation of pores in the cytoplasmic membrane. As Corynebacterium species are ubiquitous in nature and include important commensal and pathogens of mammalian organisms, secretion of bacteriocins by species of this genus could be a hitherto neglected trait with high relevance for intra- and interspecies competition and infection.ImportanceBacteriocins are antimicrobial peptides produced by bacteria to fend off competitors in ecological niches and are considered to be important factors influencing the composition of microbial communities. However, bacteriocin production by bacteria of the genus Corynebacterium has been a hitherto neglected trait, although its species are ubiquitous in nature and make up large parts of the microbiome of humans and animals. In this study, we describe and characterize a novel linaridin-family bacteriocin from Corynebacterium lactis and show its narrow spectrum activity, mainly against other actinobacteria. Moreover, we were able to extend the limited knowledge on linaridin bioactivity in general and for the first time describe the mode of action of such a bacteriocin. Interestingly, the peptide, which was named corynaridin, appears bactericidal, but without formation of pores in the bacterial membrane.

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Cutaneous abscess caused by Corynebacterium lactis in a companion dog

Cited by 6 publications

References 16 publications

Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis

Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis

Recurrent pyogranulomatous polymyositis and intramuscular abscesses secondary to Corynebacterium species in a young beagle

Identification, Characterization and Mode of Action of Corynaridin, a Novel Linaridin from Corynebacterium lactis

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