Cut-Column Pollination Method to Overcome Pollination Barrier in Phalaenopsis

Chuang, Hua-Ting; Huang, Kuang-Liang; Hsu, Shan–Te

doi:10.1007/978-1-4939-7771-0_12

Cited by 2 publications

(4 citation statements)

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“…Approximately 82% of these cultivars are tetraploid, and the rest are either triploid or aneuploid (Di et al 2013, He et al 2013, Zhuang et al 2007). Many cultivars are produced via interspecific hybridization and intergeneric hybridization (Bolaños-Villegas et al 2017, Chuang et al 2013, Yuan et al 2018. Differences in the frequency of diploidy and the size of chromosomes in Phalaenopsis species make transferring desirable genes of wild species to commercial hybrids a major challenge (Chen et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Differences in the frequency of diploidy and the size of chromosomes in Phalaenopsis species make transferring desirable genes of wild species to commercial hybrids a major challenge (Chen et al 2010). Most of the progeny produced by crosses between species differing in chromosome size are sterile (Chuang et al 2013(Chuang et al , 2014. Chromosome doubling is an effective method for circumventing hybrid sterility.…”

Section: Introductionmentioning

confidence: 99%

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Induction of 2n pollen with colchicine during microsporogenesis in <i>Phalaenopsis</i>

et al. 2022

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The induction of 2n pollen is an important technique for breeding polyploid plants. Here, we observed meiosis in the pollen mother cells (PMCs) of six Phalaenopsis cultivars and attempted to induce 2n pollen. The meiotic stage was related to flower bud length. During meiosis, Phalaenopsis cultivars with flower widths of approximately 20-40 mm and 50-60 mm had bud lengths of approximately 3-8 mm and 5-13 mm, respectively. The duration of meiosis ranged from 4.2 to 14 d. This was the first study to characterize meiosis of the PMCs of Phalaenopsis. The natural generation frequency of 2n pollen varied from 0.68% to 1.78%. Meiotic stage and colchicine concentration significantly affected the induction of 2n pollen. The most effective treatment for obtaining 2n pollen was 0.05% colchicine in the leptotene to zygotene stage for 3 d, which achieved a 2n pollen frequency of 10.04%.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of 2n pollen with colchicine during microsporogenesis in <i>Phalaenopsis</i>

et al. 2022

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“…The genus Phalaenopsis belongs to the orchid family which comprises approximately 60 species native to tropical and subtropical Asia (Chen and Tang, 2018). Phalaenopsis has a high horticultural value as potted plants or cut flowers and is widely cultivated in greenhouses (Chuang et al, 2018). Because of the great diversity in plant type and flower color, Phalaenopsis species is one of the most popular orchids in the world market (Chen and Tang, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Around one-fourth of 63 species of the genus Phalaenopsis have been popularly used for the breeding of commercial hybrids. One of the most important problems is chromosome abnormalities and the occurrence of sterility after hybridization between tetraploid varieties and diploid species (Chuang et al, 2018). Therefore, there is a need to transform diploid species to tetraploid for development of new commercial varieties for the markets (Chen and Tang, 2018).…”

Section: Introductionmentioning

confidence: 99%

In vivo polyploidy induction of Phalaenopsis amabilis in a bubble bioreactor system using colchicine

2021

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Phalaenopsis amabilis Blume var. grandiflora Bateman is economically important as cut and pot flower. Polyploidy is considered as a valuable tool in improvement and evolution of ornamental plants. Protocorm-like bodies (PLBs) of P. amabilis were cultured on Murashige and Skoog medium containing 0.20 mg L-1 IBA together with 2.00 mg L-1 KIN and 1.00 g L-1 activated charcoal and grown for a period of five months. Fully-developed plantlets from in vitro grown PLBs were immersed in a bubble reactor filled with half-strength Hoagland solution containing the antimitotic agent colchicine (0.05%, 0.10% and 0.15%, w/v) for 72 h with a few drops (1 mL of 0.1%) of octylphenoxypolyethoxyethanol or Nonidet (P-40) as a surfactant. Plantlets were aerated to prevent hypoxia. Colchicine-treated and untreated plantlets were transferred to pots for a period of 60 days. Tetraploidy was successfully induced by 0.15% colchicine. Polyploidy levels were firstly detected using flow cytometry and then confirmed by cytological and morphological observations. The chromosome number was 2n = 2x = 38 in diploids and 2n = 4x = 76 in tetraploid. Incubation of plantlets in liquid medium containing 0.15% colchicine induced the maximum recovered tetraploids with minimum frequency of survival (50%). The tetraploid plants were more compact and exhibited round and thick leaves with darker green color than diploids. Stomata size in tetraploids were larger with less density than diploids. Chloroplast number in guard cells of tetraploids was about two times more than that of control. These results indicate that induction of tetraploids are a reliable and powerful tool for generation of novel phenotypes with ornamental and horticultural value for genetic improvement and breeding. Produced tetraploids in current study have potential in the ornamental/floriculture trade.

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Cut-Column Pollination Method to Overcome Pollination Barrier in Phalaenopsis

Cited by 2 publications

References 24 publications

Induction of 2n pollen with colchicine during microsporogenesis in <i>Phalaenopsis</i>

Induction of 2n pollen with colchicine during microsporogenesis in <i>Phalaenopsis</i>

In vivo polyploidy induction of Phalaenopsis amabilis in a bubble bioreactor system using colchicine

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