Customizable, multi-functional fluorocarbon nanoparticles for quantitative in vivo imaging using 19F MRI and optical imaging

Srinivas, Mangala; Cruz, Luis J.; Bonetto, F.; Heerschap, Arend; Figdor, Carl G.; Vries, I. Jolanda M. de

doi:10.1016/j.biomaterials.2010.05.069

Cited by 127 publications

(202 citation statements)

References 31 publications

(48 reference statements)

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“…Also, the usage of iron oxide contrast at low dose is an option, especially if the signal intensity change can be correlated with its concentration. 34 In principle also other MR approaches have potential to visualize repaired bone lesions like the use of 19 F nuclei in contrast material 35 or the application of 31 P MR to differentiate between natural and synthetic hydroxyapatite. 36 In conclusion, this study reported on the first in vivo MRI of a CPC repaired lesion in bone, using a ZTE acquisition sequence and clinically approved Gd-DTPA for enhanced contrast.…”

Section: Discussionmentioning

confidence: 99%

Zero Echo Time Magnetic Resonance Imaging of Contrast-Agent-Enhanced Calcium Phosphate Bone Defect Fillers

Sun

Ventura²,

Oosterwijk³

et al. 2013

Tissue Engineering Part C: Methods

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Calcium phosphate cements (CPCs) are widely used bone substitutes. However, CPCs have similar radiopacity as natural bone, rendering them difficult to be differentiated in classical X-ray and computed tomography imaging. As conventional magnetic resonance imaging (MRI) of bone is cumbersome, due to low water content and very short T 2 relaxation time, ultra-short echo time (UTE) and zero echo time (ZTE) MRI have been explored for bone visualization. This study examined the possibility to differentiate bone and CPC by MRI. T 1 and T 2 * values determined with UTE MRI showed little difference between bone and CPC; hence, these materials were difficult to separate based on T 1 or T 2 alone. Incorporation of ultra-small particles of iron oxide and gadopentetatedimeglumine (Gd-DTPA; 1 weight percentage [wt%] and 5 wt% respectively) into CPC resulted in visualization of CPC with decreased intensity on ZTE images in in vitro and ex vivo experiments. However, these additions had unfavorable effects on the solidification time and/or mechanical properties of the CPC, with the exception of 1% Gd-DTPA alone. Therefore, we tested this material in an in vivo experiment. The contrast of CPC was enhanced at an early stage postimplantation, and was significantly reduced in the 8 weeks thereafter. This indicates that ZTE imaging with Gd-DTPA as a contrast agent could be a valid radiation-free method to visualize CPC degradation and bone regeneration in preclinical experiments.

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Section: Discussionmentioning

confidence: 99%

Zero Echo Time Magnetic Resonance Imaging of Contrast-Agent-Enhanced Calcium Phosphate Bone Defect Fillers

Sun

Ventura²,

Oosterwijk³

et al. 2013

Tissue Engineering Part C: Methods

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“…86 The importance of identical 19 F nuclei for 19 F MRI has been pointed out in previous studies, 93 and is also illustrated in Figure 1-3. A single and sharp resonance from symmetric PFCs is favourable for 19 F MRI as it can avoid the use of selective MRI pulse sequences, and thus provide images with no ghost artefacts as well as maximised signal-to-noise ratio (SNR).…”

supporting

confidence: 56%

“…A single and sharp resonance from symmetric PFCs is favourable for 19 F MRI as it can avoid the use of selective MRI pulse sequences, and thus provide images with no ghost artefacts as well as maximised signal-to-noise ratio (SNR). 86,93 PFCs are highly hydrophobic and lipophobic and therefore they are normally formulated into stable emulsions, in particular nanoemulsions, for biological applications. Through high energy processing such as microfluidisation or sonication, PFC nanoemulsions with size of 20~500 nm have been prepared by using small amounts of surfactants.…”

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confidence: 99%

Multifunctional Nanostructured Polymers for Improved 19F MRI

Wang¹

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“…In order to promote biocompatibility and cell loading efficiency, formulations of PFCs used for cell labelling and imaging generally consist of coated nanoemulsions or polymer-based nanoparticles. 10,89,90 A nanoemulsion-based formulation of PFCs was also used in the very recently published first report on 19 F-based cell imaging in humans. 39 In this study, dendritic cells (DCs) were labelled with a PFC nanoemulsion formulation, with the purpose of visualizing the DCs after intradermal administration as part of a Phase-I trial for DC-based immunotherapy of Stage-4 colorectal cancer ( Figure 5).…”

Section: Fluorine-19-based Nanoparticlesmentioning

confidence: 99%

Nanoparticles and clinically applicable cell tracking

Bernsen

Guenoun

Tiel

et al. 2015

BJR

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In vivo cell tracking has emerged as a much sought after tool for design and monitoring of cell-based treatment strategies. Various techniques are available for pre-clinical animal studies, from which much has been learned and still can be learned. However, there is also a need for clinically translatable techniques. Central to in vivo cell imaging is labelling of cells with agents that can give rise to signals in vivo, that can be detected and measured non-invasively. The current imaging technology of choice for clinical translation is MRI in combination with labelling of cells with magnetic agents. The main challenge encountered during the cell labelling procedure is to efficiently incorporate the label into the cell, such that the labelled cells can be imaged at high sensitivity for prolonged periods of time, without the labelling process affecting the functionality of the cells. In this respect, nanoparticles offer attractive features since their structure and chemical properties can be modified to facilitate cellular incorporation and because they can carry a high payload of the relevant label into cells. While these technologies have already been applied in clinical trials and have increased the understanding of cell-based therapy mechanism, many challenges are still faced.

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Customizable, multi-functional fluorocarbon nanoparticles for quantitative in vivo imaging using 19F MRI and optical imaging

Cited by 127 publications

References 31 publications

Zero Echo Time Magnetic Resonance Imaging of Contrast-Agent-Enhanced Calcium Phosphate Bone Defect Fillers

Zero Echo Time Magnetic Resonance Imaging of Contrast-Agent-Enhanced Calcium Phosphate Bone Defect Fillers

Multifunctional Nanostructured Polymers for Improved 19F MRI

Nanoparticles and clinically applicable cell tracking

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