Custodiol-N Is Superior to Custodiol® Solution in Experimental Rat Uterus Preservation

Abstract

Uterus transplantation (UTx) is the first and only available treatment for women with absolute uterine factor infertility. However, clinical application is limited by the lack of organs, ischemia/reperfusion injury, as well as immunosuppression after UTx. Several different preservation solutions are used in experimental and clinical UTx, including Custodiol® solution. Recently, the novel Custodiol-N solution was developed with superior results in organ preservation. However, the solution was not tested yet in UTx. Therefore, the aims of this study were to evaluate the effect of Custodiol-N in uterus prolonged cold preservation time (8 and 24 h), compared to Custodiol® solution. Uterus tissue samples were obtained from adult Sprague Dawley rats (n = 10/group). Cold ischemic injury was estimated by histology, including immunohistochemistry, and biochemical tissue analyses. After 8 h of cold ischemia, higher percentage of tissue edema, necrosis signs and myeloperoxidase expression, as well as lower superoxide dismutase activity were found in Custodiol® compared to Custodiol-N (p < 0.05). These differences were more pronounced after 24 h of cold preservation time (p < 0.05). This study demonstrated that Custodiol-N protects uterus grafts from cold ischemic injury better than standard Custodiol® most likely via inhibition of oxidative stress and tissue edema. It seems that iron chelators in the composition of Custodiol-N play an important protective role against cold ischemia.

Involving Animal Models in Uterine Transplantation

“…Several preservation solutions were used in animal models to improve the quality of the grafts. Custodiol-N ( 45 ) was better than Custodiol for uterine preservation in rats. Adding acetyl L-carnitine to a Histidine-Tryptophan-Ketoglutarate solution prevented the formation of free radicals ( 44 ).…”

Melatonin and Glycine Reduce Uterus Ischemia/Reperfusion Injury in a Rat Model of Warm Ischemia

“…Rat spleen tissue was used as positive control, while primary antibody was omitted as negative control. The slides were scanned using the QuPath software version 0.2.0-m5 (Belfast, Northern Ireland) [13,62] and analyzed by a blinded examiner. Results are given as the ratio of MPO-positive cells to the total number of cells (percent MPO-positive cells).…”

“…These processes increase the likelihood of short-and long-term complications, such as delayed graft function and acute or chronic rejection [11]. Different strategies to reduce IRI, such as optimization of organ perfusion and storing conditions, or the development of drugs targeting IRI, are under investigation [12,13].…”

Dietary Melatonin and Glycine Decrease Tumor Growth through Antiangiogenic Activity in Experimental Colorectal Liver Metastasis

“…For positive controls, rat intestinal and heart tissues were used for Ki67 and CD31, respectively, while for negative controls, primary antibodies were omitted. Stained slides were scanned and analyzed using the QuPath software version 0.2.0-m5 (Belfast, Northern Ireland) [26,27]. For Ki67 analysis, the number of positive cells was counted in a blinded fashion and expressed as the percentage of stained cells of total nuclei.…”