Curved and Folded Micropatterns in 3D Cell Culture and Tissue Engineering

Yilmaz, Cem Onat; Xu, Zinnia S.; Gracias, David H.

doi:10.1016/b978-0-12-800281-0.00009-9

Cited by 5 publications

(3 citation statements)

References 57 publications

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“…These conditions were perturbed during disease progression, leading to a more aggressive or debilitating prognosis. − In recent years, attention has been increasingly attracted by the micropatterning of soft material surfaces. In this way, the materials tend to mimic natural morphologies on the micro/nanometer scale. , There were numerous studies investigating the theme of 3D patterning approaches in soft materials including self-rolling, , origami-inspired, , and 3D or 4D printing. − The latter has become the last frontier and consists in biomaterials also called intelligent materials, that have, as an additional feature, the sensitivity to external stimuli. In fact, 4D printed materials can transform their structure in response to specific stimuli, such as pressure, temperature, pH, or light radiation.…”

Section: Introductionmentioning

confidence: 99%

“…In this way, the materials tend to mimic natural morphologies on the micro/nanometer scale. 22,23 There were numerous studies investigating the theme of 3D patterning approaches in soft materials including self-rolling, 24,25 origami-inspired, 26,27 and 3D or 4D printing. 28−30 The latter has become the last frontier and consists in biomaterials also called intelligent materials, that have, as an additional feature, the sensitivity to external stimuli.…”

Section: Introductionmentioning

confidence: 99%

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3D Cell Culture: Recent Development in Materials with Tunable Stiffness

Baruffaldi

Palmara

Pirri

et al. 2021

ACS Appl. Bio Mater.

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It is widely accepted that three-dimensional cell culture systems simulate physiological conditions better than traditional 2D systems. Although extracellular matrix components strongly modulate cell behavior, several studies underlined the importance of mechanosensing in the control of different cell functions such as growth, proliferation, differentiation, and migration. Human tissues are characterized by different degrees of stiffness, and various pathologies (e.g., tumor or fibrosis) cause changes in the mechanical properties through the alteration of the extracellular matrix structure. Additionally, these modifications have an impact on disease progression and on therapy response. Hence, the development of platforms whose stiffness could be modulated may improve our knowledge of cell behavior under different mechanical stress stimuli. In this review, we have analyzed the mechanical diversity of healthy and diseased tissues, and we have summarized recently developed materials with a wide range of stiffness.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

3D Cell Culture: Recent Development in Materials with Tunable Stiffness

Baruffaldi

Palmara

Pirri

et al. 2021

ACS Appl. Bio Mater.

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“…Curving and folding of tissues at multiple length scales, defined here as hierarchical, is a typical architectural phenotype found in living systems. − These geometries provide unique advantages for enhanced surface area, cell viability, and functionality. For example, the dermal-epidermal junction in human skin exhibits an undulating pattern with dermal papillae projecting into the dermis.…”

Section: Introductionmentioning

confidence: 99%

Hierarchically Curved Gelatin for 3D Biomimetic Cell Culture

Pahapale

Gao

Romer³

et al. 2019

ACS Appl. Bio Mater.

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The stiffness, microcurvature, and meso-curvature of cellular microenvironments can significantly alter cell and tissue function. However, it is challenging to produce in vitro tissue models that feature tunability in shape, stiffness, and curvature simultaneously in a high-throughput and costeffective manner. One of the significant challenges is the fragility of micropatterns in soft and biocompatible hydrogels.Here, we describe an approach that combines reflow photolithography, soft lithography, and strain engineering to create soft anatomically mimetic gelatin cell culture models. The models can be mechanically tuned to have stiffnesses as low as 400 Pa to as high as 50 kPa featuring hierarchical curvature at two length scales: the cellular length scale of 12 to 120 μm, and the mesoscale of 1−4 mm. We characterize the microstructured gels using optical microscopy and rheometry, highlighting tunability in the hierarchical curvature, modulus, and shape. Also, collagenbased gelatin offers high-level biocompatibility and bypasses the need for additional surface modification to enhance cell adhesion. We anticipate that this approach could advance anatomically accurate in vitro 3D cell culture models of relevance to biofabrication, cell biology, and drug screening.

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Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering

Bosch‐Fortea

Martı́n-Belmonte

2020

Methods in Molecular Biology

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Curved and Folded Micropatterns in 3D Cell Culture and Tissue Engineering

Cited by 5 publications

References 57 publications

3D Cell Culture: Recent Development in Materials with Tunable Stiffness

3D Cell Culture: Recent Development in Materials with Tunable Stiffness

Hierarchically Curved Gelatin for 3D Biomimetic Cell Culture

Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering

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