Curvature Sensing by the Epsin N-Terminal Homology Domain Measured on Cylindrical Lipid Membrane Tethers

Capraro, Benjamin R.; Yoon, Yonghan; Cho, Wonhwa; Baumgart, Tobias

doi:10.1021/ja907936c

Cited by 84 publications

(112 citation statements)

References 18 publications

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“…A recent review (13) concluded that hydrophobic and electrostatic membrane interactions must be in opposition for membrane curvature sensing to occur. Our study, in line with previous findings (28,30,65), shows that this is not a necessary condition. Several of the examined peptides showed strong curvature sensing despite positive interactions between interfacial lysines and the negatively charged membrane.…”

Section: Discussionsupporting

confidence: 93%

Membrane Curvature Sensing by Amphipathic Helices

Jensen

Bhatia

Jao

et al. 2011

Journal of Biological Chemistry

110

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Background: Amphipathic helices preferentially bind highly curved lipid membranes, providing a method of protein sorting. Results: Curvature sensing requires the insertion of hydrophobic residues and is modulated by electrostatic interactions. Conclusion:The relative strength of hydrophobic and electrostatic membrane interactions determines whether helix-containing proteins sense curvature. Significance: Sensing cannot be described through simple physicochemical properties but depends on the total sum of membrane interactions.

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Section: Discussionsupporting

confidence: 93%

Membrane Curvature Sensing by Amphipathic Helices

Jensen

Bhatia

Jao

et al. 2011

Journal of Biological Chemistry

110

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“…This tilt results in very high effective curvature of the proteolipid complex consisting of the inserting domain and its boundary lipids. For the ENTH domain of epsin, the adaptor protein is responsible for membrane curvature creation during endocytosis, this curvature being similar in magnitude to those created by DOPE or lysolipids (Capraro et al 2010). The interaction between the protein inserting motifs and lipid polymorphism is evident from the experiments demonstrating the effect of membrane-inserting peptides on the phase behavior of lipids.…”

Section: Protein Insertionmentioning

confidence: 92%

“…The coupling is revealed as lateral redistribution of lipids or lipid -protein complexes in a curved membrane so that the SC of the lipid bilayer approaches its geometric curvature (Sorre et al 2009;Bashkirov et al 2010;Capraro et al 2010). This redistribution leads to lowering of the membrane energy and thus "auto-tuning" of membrane shape (Fig.…”

Section: Curvature-composition Couplingmentioning

confidence: 99%

Lipid Polymorphisms and Membrane Shape

Frolov¹,

Shnyrova²,

Zimmerberg³

2011

Cold Spring Harbor Perspectives in Biology

160

113

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Morphological plasticity of biological membrane is critical for cellular life, as cells need to quickly rearrange their membranes. Yet, these rearrangements are constrained in two ways. First, membrane transformations may not lead to undesirable mixing of, or leakage from, the participating cellular compartments. Second, membrane systems should be metastable at large length scales, ensuring the correct function of the particular organelle and its turnover during cellular division. Lipids, through their ability to exist with many shapes ( polymorphism), provide an adequate construction material for cellular membranes. They can selfassemble into shells that are very flexible, albeit hardly stretchable, which allows for their far-reaching morphological and topological behaviors. In this article, we will discuss the importance of lipid polymorphisms in the shaping of membranes and its role in controlling cellular membrane morphology.

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“…The curvature-sensing ability of -arrestin, the likely adaptor protein for 2AR (Goodman et al, 1996;Traub, 2009), is currently unknown. However, other adaptor proteins such as epsin, an adaptor for ubiquitinated receptors, show strong preference for curved membranes (Capraro et al, 2010) and could affect the sorting of such receptors to the more deeply invaginated parts. A potential role for such differential targeting could be to ensure prolonged signaling from plasma membrane-localized platforms.…”

Section: Protein Purification and In Vitro Characterizationmentioning

confidence: 99%