Curvature factor and membrane solubilization, with particular reference to membrane rafts

Mrówczyńska, Lucyna; Salzer, Ulrich; Iglič, Aleš; Hägerstrand, Henry

doi:10.1042/cbi20100786

Cited by 12 publications

(13 citation statements)

References 65 publications

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“…Additionally EVs seem to be enriched in proteins and lipids associated with membrane rafts 50,52. Consistent with this, much experimental and theoretical evidence indicates the importance of membrane rafts in the process of membrane budding and vesiculation 48.…”

Section: Introductionsupporting

confidence: 53%

The role of extracellular vesicles in phenotypic cancer transformation

Ogorevc

Kralj‐Iglič²,

Veranič

2013

Radiology and Oncology

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BackgroundCancer has traditionally been considered as a disease resulting from gene mutations. New findings in biology are challenging gene-centered explanations of cancer progression and redirecting them to the non-genetic origins of tumorigenicity. It has become clear that intercellular communication plays a crucial role in cancer progression. Among the most intriguing ways of intercellular communication is that via extracellular vesicles (EVs). EVs are membrane structures released from various types of cells. After separation from the mother membrane, EVs become mobile and may travel from the extracellular space to blood and other body fluids.ConclusionsRecently it has been shown that tumour cells are particularly prone to vesiculation and that tumour-derived EVs can carry proteins, lipids and nucleic acids causative of cancer progression. The uptake of tumour-derived EVs by noncancerous cells can change their normal phenotype to cancerous. The suppression of vesiculation could slow down tumour growth and the spread of metastases. The purpose of this review is to highlight examples of EV-mediated cancer phenotypic transformation in the light of possible therapeutic applications.

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Section: Introductionsupporting

confidence: 53%

The role of extracellular vesicles in phenotypic cancer transformation

Ogorevc

Kralj‐Iglič²,

Veranič

2013

Radiology and Oncology

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“…In our experiments we used a much lower concentration in order to study cell membranes that have remained intact. Triton X-100 still penetrates cell membranes in the monomeric form [24] and also increases the formation of rafts [25]. Here we used these two agents to assess their effect on the morphology of the LSEC cell membrane.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells

et al. 2012

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Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was an inverse distribution between sieve plates and membrane rafts visualized by structured illumination microscopy and the fluorescent raft stain, Bodipy FL C5 ganglioside GM1. 7-ketocholesterol and/or cytochalasin D increased both fenestrations and lipid-disordered membrane, while Triton X-100 decreased both fenestrations and lipid-disordered membrane. The effects of cytochalasin D on fenestrations were abrogated by co-administration of Triton X-100, suggesting that actin disruption increases fenestrations by its effects on membrane rafts. Vascular endothelial growth factor (VEGF) depleted lipid-ordered membrane and increased fenestrations. The results are consistent with a sieve-raft interaction, where fenestrations form in non-raft lipid-disordered regions of endothelial cells once the membrane-stabilizing effects of actin cytoskeleton and membrane rafts are diminished.

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“…Formation of NVs is consistent with lateral distribution of membrane constituents and is reflected in the composition of NVs [37,38]. Therefore possible mechanisms of suppression of vesiculation should be considered.…”

Section: Non-specific Biophysical Mechanisms May Be Key To Manipulatimentioning

confidence: 78%

A 32-month follow-up study of nanovesicle concentrations in blood from 12 patients with gastrointestinal stromal tumour treated with imatinib

Ogorevc

Štukelj

Bedina-Zavec

et al. 2013

Biochemical Society Transactions

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Clinical studies have indicated that the NV (nanovesicle) concentration in blood samples is a potential indicator of clinical status and can be used to follow the development of the disease. For 32 months, we monitored the effect of imatinib treatment on NV concentrations in blood samples from 12 patients with GIST (gastrointestinal stromal tumour). The NV concentration before the treatment increased with respect to control by a factor of 3.5 on average (range 2.6-9.2). The first week after initiation of the treatment, the NV concentration increased considerably, by a factor of 13 on average (range 5.9-21.2), whereas on average, after 1 month, it decreased to the level of the control and remained at that level for at least 1.5 years. Recent assessment (after 2.5 years) showed a somewhat increased NV concentration, by a factor of 2 on average (range 0.7-3.9). Low NV concentrations in blood samples during the treatment reflect a favourable effect of imatinib in these patients and no remission of the disease was hitherto observed.

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Curvature factor and membrane solubilization, with particular reference to membrane rafts

Cited by 12 publications

References 65 publications

The role of extracellular vesicles in phenotypic cancer transformation

The role of extracellular vesicles in phenotypic cancer transformation

The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells

A 32-month follow-up study of nanovesicle concentrations in blood from 12 patients with gastrointestinal stromal tumour treated with imatinib

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