Curtobacterium flaccumfaciens pv. flaccumfaciens detection in bean seeds using a semi-selective culture medium

Abstract

The bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens is currently considered one of the most important bacterial bean disease in Brazil. One of the most effective control methods against this disease is the use of healthy seeds. However, no methods are known that could be routinely used to detect this bacterium in bean seeds under Brazilian condition. The aim of this work was to evaluate qualitative and quantitative detection methods for Curtobacterium flaccumfaciens pv. flaccumfaciens in naturally-infected bean seeds, and the detection of this pathogen in thirty bean seed samples, by sowing onto a semi-selective culture medium the leachate obtained from soaked bean seeds. Both the qualitative and quantitative methods were effective for detecting the presence of the bacteria in the seeds samples analysed. The qualitative method proved more practical for rotine use; of the thirty bean seed samples analyzed by this method, fifty percent were infected with Curtobacterium flaccumfaciens pv. flaccumfaciens.

First Report of Curtobacterium flaccumfaciens pv. flaccumfaciens Causing Cowpea Bacterial Wilt in Iran

“…Detection of the pathogen on infected seeds is relatively easy due to their discoloration of yellow, orange or purple depending on the pigment of the bacterial strain (EPPO ). On the other hand, an effective semi‐selective culture medium has been developed for the detection of the bacterium in seed and leaf samples (Maringoni and Camara ; Camara et al. ).…”

Pest categorisation of Curtobacterium flaccumfaciens pv. flaccumfaciens

“…Semi‐selective media for in vitro growth of C. flaccumfaciens pv flaccumfaciens are available (Mizuno and Kawai, ; Tegli et al., ; Maringoni and Camara, ; Maringoni et al., ), but methods based on isolation are quite time‐consuming and insufficiently specific (Tegli, ).…”

Seleção precoce em plantas segregantes de feijoeiro para resistência à murcha de Curtobacterium

“…flaccumfaciens (Theodoro & Maringoni, 2006). O controle preventivo desta bactéria está fundamentado no uso de cultivares resistentes, rotação de culturas e uso de sementes sadias (Maringoni & Camara, 2006;Alencar et al, 2008;Herbes et al, 2008). Entretanto, o uso de variedades resistentes constitui um dos mais importantes meios de controle de doenças de plantas cultivadas, pois, além de ser uma das medidas mais econômi-cas, não traz risco de contaminação ambiental (Galli, 2005).…”