Current View of The Mechanisms Controlling The Transcription of The TOL Plasmid Aromatic Degradation Pathways

The interplay of EIIANtr with C‐source regulation of the Pu promoter of Pseudomonas putida mt‐2

“…Moreover, the retroactivity (Jayanthi et al, ) between the plasmid‐encoded pathway and the host metabolic and regulatory network may surely involve additional players with important roles. A number of factors have been identified (Crc, IHF, Hfq, CbrAB, CyoB; Domínguez‐Cuevas and Marqués, ) and perhaps there are still others. EIIA Ntr itself is growingly recognized to interplay with more and more molecular partners (Ronneau et al, ; Muriel‐Millán et al, ) that could have direct or indirect effects in Pu output.…”

“…This in turn triggers transcription from the σ 54 ‐dependent promoter Pu and causes expression of the upper TOL operon. This signal is further propagated into the regulatory network through an intricate control circuit involving a suite of plasmid‐encoded and chromosome‐encoded factors (Domínguez‐Cuevas and Marqués, ). The key event remains however, the activation of the Pu promoter as it reflects the decision to build an energy‐expensive multi‐enzymatic complex for degradation of m ‐xylene.…”