Current vascular allograft procurement, cryopreservation and transplantation techniques in the Asan Medical Center Tissue Bank

Hwang, Shin; Bae, Ju Hee; Kim, In-Ok; Hong, Jung-Ja

doi:10.52604/alt.21.0016

Cited by 7 publications

(8 citation statements)

References 13 publications

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“…19 Portal vein stenting in small infant patients should be avoided as much as possible because the expansion of an endovascular stent is were complied with Korean legislation and conformed to ethical and safety concerns for therapeutic use. 23,24 The present study had several limitations, including its retrospective design and its inclusion of a small number of patients treated at a single center. Further studies that include larger numbers of patients are needed to validate the results of this study.…”

Section: Discussionmentioning

confidence: 99%

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Usability of intraoperative cine‐portogram during liver transplantation in young pediatric patients with biliary atresia

Namgoong

Hwang

et al. 2021

Pediatric Transplantation

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Background Pediatric patients with biliary atresia (BA) often present liver cirrhosis‐associated portal hypertension and portal vein (PV) hypoplasia. For successful liver transplantation (LT), it is essential to maintain sufficient PV inflow through stenosis‐free PV reconstruction with effective ligation of collateral veins. The aim of this study was to assess the clinical usability of intraoperative cine‐portogram (IOCP) in young pediatric patients who underwent LT for BA. Methods Medical records of pediatric patients younger than 10 years who underwent primary LT for BA from 2018 to 2020 were reviewed. Results A total of 31 patients had undergone Kasai portoenterostomy soon after birth. Their median ages at Kasai portoenterostomy and LT were 1 and 11 months, respectively. Types of LT were living‐donor LT in 13, deceased‐donor split LT in 15, and deceased‐donor whole LT in three patients. PV interposition using an iliac vein homograft was performed in 28 patients receiving partial liver grafts. Side‐to‐side PV unification venoplasty was performed in three patients undergoing whole LT. All patients underwent ligation of collateral veins. IOCP was performed in 6 (19.4%) patients. Four showed no or faint residual venous collaterals. Collateral vein embolization and endovascular stenting were performed in one patient each. PV insufficiency‐free survival rate was 100% at 1 year and 93.8% at 3 years. All patients are currently alive with a median follow‐up period of 23 months. Conclusions Intraoperative cine‐portogram can be a useful method for identification and embolization of residual portosystemic collateral veins in young pediatric patients who undergo LT for biliary atresia.

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Section: Discussionmentioning

confidence: 99%

“…All human tissues stored at the tissue bank were donated after obtaining informed consent from donors’ family members. All procedures for vascular tissue procurement and processing were complied with Korean legislation and conformed to ethical and safety concerns for therapeutic use 23,24 …”

Section: Discussionmentioning

confidence: 99%

Usability of intraoperative cine‐portogram during liver transplantation in young pediatric patients with biliary atresia

Namgoong

Hwang

et al. 2021

Pediatric Transplantation

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“…Microfractures in human arterial grafts were also described by Novotný [24]. In the clinical situation, such an event can cause early graft rupture of thawed arterial grafts [16,17,25,26]. Such events were not confirmed by us in the case of venous grafts [27].…”

Section: Discussionmentioning

confidence: 99%

Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts

Měřička

Janoušek

Benda

et al. 2021

IJMS

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The authors present their contribution to the improvement of methods suitable for the detection of the freezing and thawing damage of cells of cryopreserved venous grafts used for lower limb revascularization procedures. They studied the post-thaw viability of cells of the wall of cryopreserved venous grafts (CVG) immediately after thawing and after 24 and 48 h culture at +37 °C in two groups of six CVG selected randomly for slow thawing in the refrigerator and rapid thawing in a water bath at +37 °C. The grafts were collected from multi-organ and tissue brain-dead donors, cryopreserved, and stored in a liquid nitrogen vapor phase for five years. The viability was assessed from tissue slices obtained by perpendicular and longitudinal cuts of the thawed graft samples using in situ staining with fluorescence vital dyes. The mean and median immediate post-thaw viability values above 70% were found in using both thawing protocols and both types of cutting. The statistically significant decline in viability after the 48-h culture was observed only when using the slow thawing protocol and perpendicular cutting. The possible explanation might be the “solution effect damage” during slow thawing, which caused a gentle reduction in the graft cellularity. The possible influence of this phenomenon on the immunogenicity of CVG should be the subject of further investigations.

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“…The only substitute homograft for IVC is a cryopreserved aorta graft in our institution. However, the diameter of the aorta homograft is often less than 2 cm, thus it is often too small for replacement of the adult IVC [14]. Currently, a large-caliber synthetic vascular graft appears to be most appropriate for IVC replacement.…”

Section: Discussionmentioning

confidence: 99%

Technical refinement of inferior vena cava replacement using a synthetic vascular graft in living donor liver transplantation

Moon

Hwang

Ahn

et al. 2021

Ann Liver Transplant

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Current vascular allograft procurement, cryopreservation and transplantation techniques in the Asan Medical Center Tissue Bank

Cited by 7 publications

References 13 publications

Usability of intraoperative cine‐portogram during liver transplantation in young pediatric patients with biliary atresia

Usability of intraoperative cine‐portogram during liver transplantation in young pediatric patients with biliary atresia

Cell Viability Assessment Using Fluorescence Vital Dyes and Confocal Microscopy in Evaluating Freezing and Thawing Protocols Used in Cryopreservation of Allogeneic Venous Grafts

Technical refinement of inferior vena cava replacement using a synthetic vascular graft in living donor liver transplantation

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