Current trends in salivary gland tight junctions

Baker, Olga J.

doi:10.1080/21688370.2016.1162348

Cited by 47 publications

(38 citation statements)

References 123 publications

(173 reference statements)

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“…The morphological impairements of SMGs and PGs are relevent to their functions. TJ‐based paracellular fluid secretion largely represents the functions of salivary glands, which play a dynamic role in salivary secretion (Peppi and Ghabriel, 2004; Baker, 2016; Yano et al , 2017). The expression and organization of TJ proteins are altered during several pathological processes of xerostomia (Ewert et al , 2010; Mellas et al , 2015; Ding et al , 2017; Cong et al , 2018; Zhang et al , 2019).…”

Section: Discussionmentioning

confidence: 99%

Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes

et al. 2020

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Tight junction (TJ) plays an important role in regulating paracellular fluid transport in salivary glands; however, little is known about the involvement of TJs in diabetes salivary glands. This study aimed to investigate the alterations of TJs and their possible contribution in diabetes‐induced hyposalivation. Here, we observed that the morphologies of submandibular glands (SMGs) were impaired, characterized by enlarged acini accumulation with giant secretory granules, which were significantly reduced in atrophic ducts in SMGs of db/db mice, a spontaneous model of type‐2 diabetes. However, the secretory granules were increased and scattered in the acini of diabetes parotid glands (PGs). Other ultrastructural damages including swollen mitochondria, expansive endoplasmic reticulum, and autophagosomes were observed in the diabetes group. The levels of TJ proteins including claudin‐1 (Cldn1) and claudin‐3 (Cldn3) were increased, whereas those of claudin‐4 (Cldn4), occludin (Ocln), and zonula occludens‐1 (ZO‐1) were decreased in SMGs of db/db mice. Higher Cldn1 and Cldn3 and lower claudin‐10 (Cldn10) and Ocln levels were observed in PGs of diabetes mice. Taken together, the structures of SMGs and PGs were impaired in diabetes mice, and the disruption of TJ integrity in both SMGs and PGs may contribute to diabetes‐induced hyposalivation.

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Section: Discussionmentioning

confidence: 99%

Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes

et al. 2020

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“…However, these proteins are critical for salivary gland function as they allow unidirectional fluid secretion as well as maintain a fence between apical and basolateral compartments for secretory function and need to be better understood. [33][34][35] A limitation of this study is that we only studied the scaffold protein, ZO-1, which links transmembrane tight junction proteins to the cytoskeleton. [36][37][38][39] Therefore, future studies including the transmembrane proteins (i.e., occludin, claudins, junctional adhesion molecules, ticellulin, and Coxsackieadenovirus receptors) will be necessary to confirm that tight junction proteins are completely lost after irradiation of the glands.…”

Section: Discussionmentioning

confidence: 99%

Post-Irradiated Human Submandibular Glands Display High Collagen Deposition, Disorganized Cell Junctions, and an Increased Number of Adipocytes

Nam

Maruyama

Trump

et al. 2016

J Histochem Cytochem.

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Salivary glands are vital for maintaining oral health. Head and neck radiation therapy is one of the most common causes of salivary gland hypofunction. Little is known about the structural changes that occur in salivary glands after radiation therapy. The aim of this study is to understand the structural changes that occur in post-irradiated human (submandibular gland [SMG]) as compared with untreated ones. We determined changes in epithelial polarity, presence of collagen deposition, and alteration in adipose tissue. We used formalin-fixed paraffin-embedded human SMG from two female subjects exposed to head and neck irradiation. We utilized hematoxylin and eosin staining and Masson’s Trichrome staining. The immunostained tissue sections were examined using confocal microscopy. The number and size of adipocytes per tissue section were calculated using ImageJ, Prism, and SPSS software. Post-irradiated human SMG displayed high collagen deposition, disorganized cell junctions, and an increased number of adipocytes as compared with non-irradiated controls. These findings are important to improve our understanding of the individual risk and variation in radiation-related salivary gland dysfunction.

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“…336 Several authors reported previously about TJ organization and expression in salivary epithelium cell models and salivary gland tissue. 336,337,338,339 A major topic of several studies is the relationship between the expression of different claudins and the corresponding barrier functionality, especially in immortalized rat salivary gland epithelial cell lines such as SMIE, SMG-C6, Par-C10 or Par-C5 (Table 4). In more detail, SMIE cells were reported to have a limited claudin expression profile, which was in line with their low barrier function, indicated by low TEER and high dextran flux.…”

Section: Tight Junctions (Tjs)mentioning

confidence: 99%

Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties

et al. 2018

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Understanding the function of oral mucosal epithelial barriers is essential for a plethora of research fields such as tumor biology, inflammation and infection diseases, microbiomics, pharmacology, drug delivery, dental and biomarker research. The barrier properties are comprised by a physical, a transport and a metabolic barrier, and all these barrier components play pivotal roles in the communication between saliva and blood. The sum of all epithelia of the oral cavity and salivary glands is defined as the blood-saliva barrier. The functionality of the barrier is regulated by its microenvironment and often altered during diseases. A huge array of cell culture models have been developed to mimic specific parts of the blood-saliva barrier, but no ultimate standard in vitro models have been established. This review provides a comprehensive overview about developed in vitro models of oral mucosal barriers, their applications, various cultivation protocols and corresponding barrier properties.

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Current trends in salivary gland tight junctions

Cited by 47 publications

References 123 publications

Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes

Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes

Post-Irradiated Human Submandibular Glands Display High Collagen Deposition, Disorganized Cell Junctions, and an Increased Number of Adipocytes

Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties

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