Current trends in biosensors for the detection of cattle diseases worldwide

Nehra, Anuj; Kundu, R.S.; Ahlawat, Sweeti; Singh, Krishna Pal; Karki, Kshitij; Lather, Aryan Singh; Poonia, Kanika; Budania, Savita; Kumar, Vanita

doi:10.1016/j.biosx.2023.100355

Cited by 2 publications

(1 citation statement)

References 89 publications

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“…Immune deficiencies are linked to disorders in the repair of DNA strand breaks, leading to disturbances in the integrity and stability [105]. According to Nehra et al [106], the rapid spread of viral diseases in cattle should be addressed by implementing various methods, technologies, and procedures to prevent the expansion of viruses and minimize negative health consequences. Hence, our study is a valuable contribution to the dissemination of information on the detection of latent genomic instability in cows with BVD.…”

Section: Discussionmentioning

confidence: 99%

Identification of Genomic Instability in Cows Infected with BVD Virus

Kępka,

Wójcik,

Wysokińska

2023

Animals

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An important factor for dairy cattle farmers is the profitability of cattle rearing, which is influenced by the animals’ health and reproductive parameters, as well as their genomic stability and integrity. Bovine viral diarrhea (BVD) negatively affects the health of dairy cattle and causes reproductive problems. The aim of the study was to identify genomic instability in cows with reproductive disorders following infection with the BVD virus. The material for analysis was peripheral blood from Holstein-Friesian cows with reproductive problems, which had tested positive for BVD, and from healthy cows with no reproductive problems, which had tested negative for BVD. Three cytogenetic tests were used: the sister chromatid exchange assay, fragile sites assay, and comet assay. Statistically significant differences were noted between the groups and between the individual cows in the average frequency of damage. The assays were good biomarkers of genomic stability and enabled the identification of individuals with an increased frequency of damage to genetic material that posed a negative impact on their health. The assays can be used to prevent disease during its course and evaluate the genetic resistance of animals. This is especially important for the breeder, both for economic and breeding reasons. Of the three assays, the comet assay proved to be the most sensitive for identifying DNA damage in the animals.

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Section: Discussionmentioning

confidence: 99%

Identification of Genomic Instability in Cows Infected with BVD Virus

Kępka,

Wójcik,

Wysokińska

2023

Animals

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Measurement of Human and Bovine Exhaled Breath Condensate pH Using Polyaniline-Modified Flexible Inkjet-Printed Nanocarbon Electrodes

Jacobs,

Prete,

Lesch

et al. 2024

ACS Omega

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The collection, processing, and electrochemical analysis of exhaled breath condensate (EBC) from healthy human and animal subjects is reported on. EBC is a biospecimen potentially rich in biomarkers of respiratory disease. The EBC pH was analyzed potentiometrically using a disposable polyaniline (PANI)-modified inkjet-printed (IJP) carbon electrode. Comparison measurements were performed using a commercial screenprinted carbon (SPC) electrode. The PANI-modified electrodes exhibited reproducible and near-Nernstian responses for pH values between 2 and 9 with slopes from −50 to −60 mV/dec. The PANI-modified IJP carbon electrode exhibited a faster response time and superior reproducibility to the modified SPC electrode. In proof-of-concept studies, the healthy human EBC pH was found to be 6.57 ± 0.09 and the healthy bovine EBC pH was 5.9 ± 0.2. All pH determined using the PANI-modified electrodes were in good agreement with the pH determined using a micro glass pH electrode. An RTube device was used to collect EBC from humans while a modified device was used to collect EBC from calves in the field. EBC volumes of 0.5−2 mL for 5−6 min of tidal breathing were collected from healthy animals. The pH of EBC from healthy calves (17 animals) depends on their age from 1 to 9 weeks with values ranging from 5.3 to 7.2. A distinct alkaline shift was observed for many animals around 20 days of age. The bovine EBC pH also depends on the ambient temperature and humidity at the time of collection. The results indicate that the PANI-modified IJP carbon electrodes outperform commercial SPC and provide reproducible and accurate measurement of pH across various biospecimen types.

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Current trends in biosensors for the detection of cattle diseases worldwide

Cited by 2 publications

References 89 publications

Identification of Genomic Instability in Cows Infected with BVD Virus

Identification of Genomic Instability in Cows Infected with BVD Virus

Measurement of Human and Bovine Exhaled Breath Condensate pH Using Polyaniline-Modified Flexible Inkjet-Printed Nanocarbon Electrodes

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