Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals

Yasin, Rohani Md; Zin, Norni Mohd; Hussin, Azura; Nawi, Salbiah; Hanapiah, Suhailah Md; Wahab, Zubaidah Abdul; Raj, Ganeswarie; Shafie, Norazita; Peng, Ng P; Chu, Keah K.; Aziz, Muhhamad Nazri; Maning, Nurahan; Mohamad, Jamilah Said; Benjamin, Adom; Salleh, Mohd. Azmi bin Mohd.; Zahari, Sabarina Sabihah; Francis, A.E.; Ahmad, Norazah; Karunakaran, Rina

doi:10.1016/j.vaccine.2011.06.004

Cited by 22 publications

(20 citation statements)

References 42 publications

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“…Other common serotypes being 19A, 14 and 6B [10], [26], [27] This observation also agrees with serotypes reported from other with data from Association of South East Asian Nations (ASEAN) isolates deposited on the MLST database (23F n = 51 (14%), 19A n = 44 (12%), 19F n = 40 (11%)). In this study ST236 contributed to the prevalence of serotype 19F (Table 1), this profile is that of the internationally disseminated multiply resistant clone known as Taiwan 19F -14 (ST236) [28] which is common in many parts of the world, including South East Asia, however isolates in our study were penicillin non-susceptible, erythromycin-resistant isolates.…”

Section: Discussionsupporting

confidence: 90%

Novel Clones of Streptococcus pneumoniae Causing Invasive Disease in Malaysia

et al. 2014

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Although Streptococcus pneumoniae is a leading cause of childhood disease in South East Asia, little has previously been reported regarding the epidemiology of invasive pneumococcal disease in Malaysia and very few studies have explored pneumococcal epidemiology using multilocus sequence typing (MLST). Here we describe serotype, multilocus sequence type (ST), and penicillin susceptibility of thirty pneumococcal invasive disease isolates received by the University of Malaya Medical Centre between February 2000 and January 2007 and relate this to the serotypes included in current pneumococcal conjugate vaccines. A high level of diversity was observed; fourteen serotypes and 26 sequence types (ST), (11 of which were not previously described) were detected from 30 isolates. Penicillin non-susceptible pneumococci accounted for 33% of isolates. The extent of molecular heterogeneity within carried and disease-causing Malaysian pneumococci remains unknown. Larger surveillance and epidemiological studies are now required in this region to provide robust evidence on which to base future vaccine policy.

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Section: Discussionsupporting

confidence: 90%

Novel Clones of Streptococcus pneumoniae Causing Invasive Disease in Malaysia

et al. 2014

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“…In Malaysia, we have observed a rise in penicillin-resistant S. pneumoniae (PRSP) from 0.8% in 1988 [4] to 7.0% in 1995 [5] and 25.54% in 2009 [6]. Another study in Malaysia [7] has reported higher resistant rate to other common oral antibiotics in comparison to another study [5] whereby resistance to erythromycin has increased from 1.1% to 33.33% and clindamycin from 0.4% to 18.08%.…”

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confidence: 99%

Evolution of Erythromycin Resistance among <i>Streptococcus pneumoniae</i> Isolates in Malaysia from 2005 and 2010

Arushothy¹,

Ahmad²,

Yassin³

2016

JBM

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Objectives: This study focuses on the antibiotic susceptibility pattern and distribution of the ermB and mefA virulence genes among the Streptococcus pneumoniae due to an increase in erythromycin resistance in S. pneumoniae worldwide. Methodology: We investigated 255 clinical isolates collected from 2005-2010 to determine the serotype distribution and resistance to erythromycin in comparison to penicillin, clindamycin, clarithromycin, azithromycin, and trimethoprim sulfamethoxazole. Multiplex PCR was carried out to detect erythromycin resistance genes (ermB and mefA). Results: There were 146 (57.3%) isolates resistant to erythromycin. MIC90 for erythromycin is at >256 mg/L and MIC50 is at 16 mg/L. The ermB gene was detected in 25.3% of the erythromycin-resistant isolates and mefA gene was detected in 50.7% of the isolates. The four most common serotypes encountered are 19F, 19A, 23F and 14. The serotype distribution among the erythromycin resistant isolates was 19F (42.0%) followed by serotype 19A (11.3%), serotype 23F (9.2%) and serogroup 14 (7.0%). Conclusion: In conclusion, there is a significant rise in erythromycin resistance among the Malaysian pneumococcal isolates. The emergence of serotype 19A together with increasing prevalence of resistance to macrolide warrants for a more extensive surveillance study.

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“…Pneumococcal capsular vaccines (PCV7 and PCV13) protect against disease and nasopharyngeal carriage due to the serotypes included in the vaccine formulations [1], [15], [17], [19]. Nevertheless, surveillance of serotype prevalence patterns is very important since the serotypes responsible for invasive disease can change over time [10], [16], [18], [20], [23]. The recent availability of sequence information of all serotypes has led to an interest in molecular typing methods.…”

Section: Discussionmentioning

confidence: 99%

“…While the results of many studies previously demonstrated that the multiplex PCR was highly accurate, fast and cost-effective method [2]–[3], [10], [25], [27], there is yet no manufactured multiplex PCR system commercially available for determination of the most invasive serogroups/types targeted by PCV13. In its present form, our multiplex PCR assay could be manufactured as a commercial typing kit for rapid screening of pneumococcal isolates.…”

Section: Discussionmentioning

confidence: 99%

“…At present, different 46 serogroups and 93 serotypes including the recently discovered 6D and 11E [4]–[5] have been described [6]–[8]. The distribution of these serogroups/types shows variation based on age, time and geography [9], [10]. Despite the large variety of capsular types, only a small number of serotypes was recovered from invasive pneumococcal diseases worldwide [11]–[13].…”

Section: Introductionmentioning

confidence: 99%

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One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

Coşkun-Ari¹,

Güldemir²,

Durmaz

2012

PLoS ONE

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The life-threatening illnesses caused by Streptococcus pneumoniae have been declined significantly after the use of pneumococcal conjugate vaccines. Continuous monitoring of the vaccine serogroups/types is necessary to follow the changing epidemiology of invasive pneumococcal diseases. Recently, the sequential multiplex PCR approach, which uses several different sets of reactions, has been commonly adopted for determining capsular serogroups/types of S. pneumoniae isolates. In our study, we focused on development of a one-step multiplex PCR assay detecting all 1, 3, 4, 5, 6A/B, 7F, 9V, 14, 18C, 19A, 19F and 23F serogroups/types targeted by PCV13. The content of multiplex PCR mix and the cycling conditions were optimized in a manner that allowed rapid and accurate serotyping of a pneumococcal isolate by performing only a single amplification reaction. In our study of 182 clinical isolates, the one-step multiplex PCR assay exhibited 100% sensitivity and specificity, suggesting that its utilization can significantly reduce the use of traditional antiserum method requiring expensive reagents.

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Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals

Cited by 22 publications

References 42 publications

Novel Clones of Streptococcus pneumoniae Causing Invasive Disease in Malaysia

Novel Clones of Streptococcus pneumoniae Causing Invasive Disease in Malaysia

Evolution of Erythromycin Resistance among <i>Streptococcus pneumoniae</i> Isolates in Malaysia from 2005 and 2010

One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

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Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals

Cited by 22 publications

References 42 publications

Novel Clones of Streptococcus pneumoniae Causing Invasive Disease in Malaysia

Novel Clones of Streptococcus pneumoniae Causing Invasive Disease in Malaysia

Evolution of Erythromycin Resistance among &lt;i&gt;Streptococcus pneumoniae&lt;/i&gt; Isolates in Malaysia from 2005 and 2010

One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

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Evolution of Erythromycin Resistance among <i>Streptococcus pneumoniae</i> Isolates in Malaysia from 2005 and 2010