Current tools for skin imaging and analysis

Cal, Krzysztof; Stefanowska, Justyna; Żakowiecki, Daniel

doi:10.1111/j.1365-4632.2009.04198.x

Cited by 12 publications

(6 citation statements)

References 27 publications

(54 reference statements)

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“…The data resembles an ultrasound image with a slightly better resolution, but is still too low for visualizing cell morphology. 20,21 RCM was the first in vivo tool that offered the possibility to study the skin at a microscopic resolution approaching histological detail. 22 This technique utilizes the distribution of naturally occurring refractive index variations within the skin to provide a source of contrast.…”

Section: Discussionmentioning

confidence: 99%

In vivo fluorescence confocal microscopy: indocyanine green enhances the contrast of epidermal and dermal structures

Skvara¹,

Kittler²,

Schmid³

et al. 2011

J. Biomed. Opt.

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Section: Discussionmentioning

confidence: 99%

In vivo fluorescence confocal microscopy: indocyanine green enhances the contrast of epidermal and dermal structures

Skvara¹,

Kittler²,

Schmid³

et al. 2011

J. Biomed. Opt.

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“…A number of non‐invasive imaging techniques have been developed over the years to provide an objective evaluation of healthy and diseased human skin , but the design of quantitative technologies to measure the surface and volume of the skin remains an ongoing challenge. In order to monitor skin changes over time, or before and after intervention, the dermatologist traditionally has had only his eyes, his memory and at best a two dimensional (2D) digital camera.…”

mentioning

confidence: 99%

Quantification of skin lesions with a 3D stereovision camera system: validation and clinical applications

Skvara

Burnett

Jones

et al. 2012

Skin Research and Technology

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“…Raman spectroscopy and other infrared methods are optical technique that provide information about the vibrational levels of the molecules and are widely used in analytical, qualitative, and quantitative studies. From these techniques, molecular structure and material composition are encoded by changes in observed frequencies through changes in light scattering . Thus, the Raman spectra can also reflect changes in the environment of the molecules and is therefore useful in intramolecular and intermolecular studies.…”

Section: Discussionmentioning

confidence: 99%

“…From these techniques, molecular structure and material composition are encoded by changes in observed frequencies through changes in light scattering. [20][21][22][23][24][25] Thus, the Raman spectra can also reflect changes in the environment of the molecules and is therefore useful in intramolecular and intermolecular studies.…”

Section: Discussionmentioning

confidence: 99%

Are metabolites of Fusarium oxysporum responsible for fungal skin invasion? A morphological and Raman spectroscopy monitoring

Melo

Svidzinski

Zapater

et al. 2014

J Raman Spectroscopy

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Fusarium is an emerging pathogen with high levels of morbidity and mortality. A significant tissue response is observed in infected patients, and the condition has been associated with the production of toxic metabolites. The aim of the present study was to identify a major fraction of crude metabolic extract of Fusarium oxysporum and investigate its effects on the skin of healthy rats. Fraction F1 was obtained from the cultivation of F. oxysporum in Czapek–Dox. In the treatment groups, fraction F1 (0.05 mg/ ml) was injected intradermally, while (50 µl) 0.9% of saline solution was injected in the control groups. The animals were killed 3, 6, 12, and 24 h after inoculation. The skin was fixed for inclusion in paraffin to obtain histological sections and stained with hematoxylin and eosin, Sirius red, and terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling. Samples were analyzed using Fourier transform Raman spectroscopy. The tissue reactions were classified and compared over time and by treatment. In the treatment group, inflammatory reaction peaked at 6 h, being classified as moderate, with infiltrate composed mainly of neutrophils. terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining was negative. The area occupied by types I and III collagen in the treatment group increased over time. There was a change in the area occupied by amide I and the ratios of the –CH2 and –CH3 molecules. It can be argued that the fraction F1 contains elements that contribute to the invasion of Fusarium in the skin, destructurizing the organization of the extracellular matrix. Copyright © 2014 John Wiley & Sons, Ltd.

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Current tools for skin imaging and analysis

Cited by 12 publications

References 27 publications

In vivo fluorescence confocal microscopy: indocyanine green enhances the contrast of epidermal and dermal structures

In vivo fluorescence confocal microscopy: indocyanine green enhances the contrast of epidermal and dermal structures

Quantification of skin lesions with a 3D stereovision camera system: validation and clinical applications

Are metabolites of Fusarium oxysporum responsible for fungal skin invasion? A morphological and Raman spectroscopy monitoring

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