Current technological advances in mapping new DNA modifications

Amouroux, Rachel; McEwen, Kirsten; Hájková, Petra

doi:10.1016/j.ddmod.2014.05.003

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Methods Involving Dna‐enrichment Strategies1

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2017

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“…Similarly, 5hmC can be modified to CMS by treatment with bisulfite and immunoprecipitated by a CMS‐specific antibody for subsequent analysis, thereby offering more sensitive and specific enrichment . However, the disadvantages of hMeDIP include its dependence on 5hmC density, its inability to give single‐base resolution, and its off‐target affinity of the antibody for simple repeats and CA dinucleotides .…”

Section: Methods Involving Dna‐enrichment Strategiesmentioning

confidence: 99%

Quantitation and mapping of the epigenetic marker 5‐hydroxymethylcytosine

Qing

Tian

et al. 2017

BioEssays

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We here review primary methods used in quantifying and mapping 5-hydroxymethylcytosine (5hmC), including global quantification, restriction enzyme-based detection, and methods involving DNA-enrichment strategies and the genome-wide sequencing of 5hmC. As discovered in the mammalian genome in 2009, 5hmC, oxidized from 5-methylcytosine (5mC) by ten-eleven translocation (TET) dioxygenases, is increasingly being recognized as a biomarker in biological processes from development to pathogenesis, as its various detection methods have shown. We focus in particular on an ultrasensitive single-molecule imaging technique that can detect and quantify 5hmC from trace samples and thus offer information regarding the distance-based relationship between 5hmC and 5mC when used in combination with fluorescence resonance energy transfer.

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Section: Methods Involving Dna‐enrichment Strategiesmentioning

confidence: 99%