Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

Parys, Wioletta; Dołowy, Małgorzata; Pyka, Alina

doi:10.3390/pr9071100

Cited by 3 publications

(3 citation statements)

References 86 publications

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“…The development and validation of rapid and reliable methods for the determination of ecdysterone and turkesterone in DSs for professional athletes would be useful for future doping investigations [50]. High-performance liquid chromatography has proven to be a versatile, rapid, sensitive, and reproducible technique for both identification and quality control of doping substances [35,[51][52][53]. In order to enhance future doping analysis, it is advisable to optimize the HPLC method for UHPLC-MS.…”

Section: Methods Application-analysis Of Dietary Supplementsmentioning

confidence: 99%

Development and Validation of High-Performance Liquid Chromatography for Identification and Quantification of Phytoecdysteroids Ecdysterone and Turkesterone in Dietary Supplements

Todorova

Ivanov

Karcheva-Bahchevanska

et al. 2023

Processes

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Phytoecdysteroids are a group of naturally occurring ecdysteroid hormones found in certain plant families and used for centuries for their adaptogenic, tonifying, and antioxidant properties. Ecdysterone and turkesterone are the dominant phytoecdysteroids found in the plant species Rhaponticum carthamoides Willd., Cyanotis arachnoidea C.B.Clarke and Ajuga turkestanica (Regel) Briq., and have been recognized for their adaptogenic potential and ability to enhance physical performance. However, over the past few years, supplementation with ecdysterone by professional athletes has raised some concerns about its safety and quality, leading to its inclusion in the World Anti-Doping Agency monitoring program. This study proposes a simple and reliable method for quality control of ecdysterone- and turkesterone-containing products using high-performance liquid chromatography (HPLC) analysis. The HPLC method is based on reversed-phase chromatography and gradient elution to achieve a superior separation of phytoecdysteroids. The method has been shown to qualify both phytoecdysteroids at low concentrations such as 10.98 μg/mL for ecdysterone and 11.43 μg/mL for turkesterone. This method could be successfully used in research programs and routine quality control of dietary supplements to ensure their safety in professional athletes’ nutrition.

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Section: Methods Application-analysis Of Dietary Supplementsmentioning

confidence: 99%

Development and Validation of High-Performance Liquid Chromatography for Identification and Quantification of Phytoecdysteroids Ecdysterone and Turkesterone in Dietary Supplements

Todorova

Ivanov

Karcheva-Bahchevanska

et al. 2023

Processes

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“…The main application area of GC is in the studies of food and edible plants, where a number of groups of compounds are analyzed, including pesticides, endocrine-disrupting chemicals, estrogens, fatty acids, etc. [70]. Balsamic vinegar samples are typically highly complex mixtures containing hundreds of compounds, while most of them are volatile.…”

Section: Separation Methods For Characterization Of Balsamic Vinegars...mentioning

confidence: 99%

Characterization of Balsamic Vinegars Using High-Performance Liquid Chromatography and Gas Chromatography

Kašpar

Česla

2022

Applied Sciences

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Vinegars are natural products manufactured by two-step fermentation. One of the most famous is balsamic vinegar, especially that produced in Modena, Italy. Its unique production process positively distinguishes it from other vinegars. There are basically three types of balsamic vinegar: common balsamic vinegar, Balsamic vinegar of Modena, and Traditional balsamic vinegar of Modena. The chemical analysis of these vinegars is mainly carried out by using gas or liquid chromatography, often coupled to mass spectrometric detection. Although gas chromatography is generally used for the determination of the overall profile of volatile organic compounds, furfurals, phenolic compounds, and organic acids, high-performance liquid chromatography is typically applied for the determination of amino acids, sugars, and polyphenols. The two complementary techniques, the combination of which is useful for the detailed characterization of balsamic vinegars, are reviewed and discussed in this article.

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“…The origin of the analyzer, the simplicity with which the various methods can be combined, the sensitivity of the required results, and the availability of the equipment should all factor into the selection of the best combination methodology. The flavonoids and phenolic acids present in Flos Lamii albi were analyzed using high-performance liquid chromatography and high-performance thin-layer chromatography [26]. [27].…”

Section: Table 2 Hyphenated Technique: Coupling Of Some Chromatograph...mentioning

confidence: 99%

An Overview- Advances in Chromatographic Techniques in Phytochemistry

Vij,

Pathania

2023

E3S Web Conf.

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The basic idea of the Chromatographic process is that the distinct compounds have different properties like solubility, adsorption, ion exchange, and affinity that can be regulated through various separation mechanisms causing the analytes to travel through the stationary phase at different speeds, resulting in their separation from a complex mixture. The Chromatographic techniques have been explored as multidimensional, which has been growing and evolving due to continuous advancements in technology allowing it to meet the upcoming challenges and analytical demands in various scientific scenarios including academics and industries. Phytochemistry is one of the oldest core areas of research in science where one of the major challenges in drug discovery from natural sources is the identification and isolation of closely related active molecules within these complex to the observed biological activities therefore there is utmost need to better understand their intrinsic complexity and exploit their vast commercial potential. Nowadays the hyphenated techniques with one component as the chromatographic technique have been widely explored as inevitable analytical tools for early detection and identification of bioactive compounds and driving forces in the evolution and discoveries in phytochemistry from crude plant extracts that boosted mainly due to rapid technological advancements in instrumentation. It’s also significant as an intellectual method for phytotherapeutic quality control and regulation. In the present review, we have discussed the advances and insight of chromatographic techniques which can be explored for comprehensive chemical profiles of herbal medicine preparations or extracts in phytochemistry. The review provides a systematic update of the recent advancement and published approaches to methodology in phytochemistry.

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Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

Cited by 3 publications

References 86 publications

Development and Validation of High-Performance Liquid Chromatography for Identification and Quantification of Phytoecdysteroids Ecdysterone and Turkesterone in Dietary Supplements

Development and Validation of High-Performance Liquid Chromatography for Identification and Quantification of Phytoecdysteroids Ecdysterone and Turkesterone in Dietary Supplements

Characterization of Balsamic Vinegars Using High-Performance Liquid Chromatography and Gas Chromatography

An Overview- Advances in Chromatographic Techniques in Phytochemistry

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