Current status on the development of detection methods for genetically modified plants

김재환,; 김영록,; 김해영,

doi:10.5010/jpb.2011.38.2.143

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…The multiplex detection methods for universal transgenic genes (P-35S, P-RbcS4, T-nos, T-35S, T-pinII, T-E9, T-AHASL, T-ORF23) of 24 LM soybeans approved in many countries had been developed [23]. These methods can be used as primary screening tools to discriminate suspicious samples whether they contain LMO or not, though further analysis is needed to verify event from LMO containing samples and several multiplex PCR methods of LM soybeans were reported [4,5]. As the LM crop market has been growing, new LM soybean events have been continuously developed and traded.…”

Section: Discussionmentioning

confidence: 99%

“…In the recent report of KBCH, 17 single events and 10 stack traits of LM soybean were approved in Republic of Korea [3]. Among approved 17 single events, 11 events have been used for simultaneous detection methods [4,5], and CV127, MON87705, FG72 and MON87701 were employed to develop an efficient multiplex PCR method in this study. CV127 is an event developed by BASF and resistant to imidazolinone herbicides due to insertion of CSR1 gene from Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

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Development and application of a novel multiplex PCR method for four living modified soybeans

Choi

Seol

et al. 2018

Appl Biol Chem

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Since the early 1990s when the first commercialization of living modified organism (LMO), LMO has been developed to improve nutrient quality and productivity of crops. As the self-sufficiency rate of soybean has gradually decreased in South Korea, most of soybeans have been imported. The cultivation and trade of LM crops are regulated in many countries and authorizations for the use are mandatory in most. In South Korea, the cultivation of LM crop is not allowed and unintentional release of LMO into the natural environment is prohibited. In this study, we developed a novel multiplex PCR method for four LM soybean events (CV127, MON87705, FG72 and MON87701) which were approved recently in South Korea. Multiplex PCR primers were designed for PCR amplification of four LMO event-specific fragments, and we analyzed 41 environmental monitoring samples to confirm the efficiency of this method. These results indicated that the multiplex PCR detection method is sufficient for four LM soybeans found in the natural environment. Based on our finding, we suggest that the new technique may be useful as a lead tool for the development of a detection method for various LMO/GMOs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and application of a novel multiplex PCR method for four living modified soybeans

Choi

Seol

et al. 2018

Appl Biol Chem

View full text Add to dashboard Cite

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Detection of LM canola with tolerance to glyphosate and glufosinate-ammonium via the Environmental monitoring in South Korea

Shin

Moon

et al. 2016

J Plant Biotechnol

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Living modified (LM) crops are imported each year to South Korea as food and feeds, LM canola being one of the imported crops. The cultivation of LM crops is not permitted in South Korea but the import of these crops is increasing. In this study, we surveyed the environmental risk of imported LM canola at 9 provinces, from March 2009 to June 2013. Monitoring of canola was conducted around feed factories, roadsides, harbors, farmhouses, and flower festival regions. From the total of 595 canola samples collected from 1850 monitoring sites, we identified 6 LM canola samples. The LM canola samples were subjected to protein and DNA based analysis. PCR analyses using approved 5 single event primers (T45, MS8, RT73, Rf3 and Topas 19-2) revealed that two crops were glyphosate-resistant LM canolas, and four were glufosinate-resistant LM canolas. This study suggested that environmental monitoring is a useful research tool to manage LM crops unintentionally introduced into the environment in South Korea. This result can be used as a basis for future post-management of canola crops.

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Current status on the development of detection methods for genetically modified plants

Cited by 2 publications

References 23 publications

Development and application of a novel multiplex PCR method for four living modified soybeans

Development and application of a novel multiplex PCR method for four living modified soybeans

Detection of LM canola with tolerance to glyphosate and glufosinate-ammonium via the Environmental monitoring in South Korea

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