Current status of the application of gene editing in pigs

Tanihara, Fuminori; Hirata, Maki; Otoi, Takeshige

doi:10.1262/jrd.2021-025

Cited by 20 publications

(17 citation statements)

References 151 publications

(205 reference statements)

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“…CRISPR/Cas9 can also be used to improve animal and plant breeding in agricultural production. It functions well in improving plant breeding in terms of yield, resistance, and quality [ 32 , 33 ]. In 2013, the Gao, C.X.…”

Section: Resultsmentioning

confidence: 99%

The Bibliometric Landscape of Gene Editing Innovation and Regulation in the Worldwide

Wei

Liu

et al. 2022

Cells

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Gene editing (GE) has become one of the mainstream bioengineering technologies over the past two decades, mainly fueled by the rapid development of the CRISPR/Cas system since 2012. To date, plenty of articles related to the progress and applications of GE have been published globally, but the objective, quantitative and comprehensive investigations of them are relatively few. Here, 13,980 research articles and reviews published since 1999 were collected by using GE-related queries in the Web of Science. We used bibliometric analysis to investigate the competitiveness and cooperation of leading countries, influential affiliations, and prolific authors. Text clustering methods were used to assess technical trends and research hotspots dynamically. The global application status and regulatory framework were also summarized. This analysis illustrates the bottleneck of the GE innovation and provides insights into the future trajectory of development and application of the technology in various fields, which will be helpful for the popularization of gene editing technology.

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Section: Resultsmentioning

confidence: 99%

The Bibliometric Landscape of Gene Editing Innovation and Regulation in the Worldwide

Wei

Liu

et al. 2022

Cells

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“…The net effects of internal factors might be of special relevance for evaluating the welfare of genetically modified rodents (and other species, such as pigs, Perleberg et al, 2018;Tanihara et al, 2021). Providing external factors, in particular management and housing conditions, may be crucial in alleviating the welfare consequences of the induced genetic modifications.…”

Section: Strategies To Improve Welfare Based On Dawconmentioning

confidence: 99%

A dynamic concept of animal welfare: The role of appetitive and adverse internal and external factors and the animal’s ability to adapt to them

2022

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Animal welfare is a multifaceted issue that can be approached from different viewpoints, depending on human interests, ethical assumptions, and culture. To properly assess, safeguard and promote animal welfare, concepts are needed to serve as guidelines in any context the animal is kept in. Several different welfare concepts have been developed during the last half decade. The Five Freedoms concept has provided the basis for developing animal welfare assessment to date, and the Five Domains concept has guided those responsible for safeguarding animal welfare, while the Quality of Life concept focuses on how the individual perceives its own welfare state. This study proposes a modified and extended version of an earlier animal welfare concept - the Dynamic Animal Welfare Concept (DAWCon). Based on the adaptability of the animal, and taking the importance of positive emotional states and the dynamic nature of animal welfare into account, an individual animal is likely in a positive welfare state when it is mentally and physically capable and possesses the ability and opportunity to react adequately to sporadic or lasting appetitive and adverse internal and external stimuli, events, and conditions. Adequate reactions are elements of an animal’s normal behavior. They allow the animal to cope with and adapt to the demands of the (prevailing) environmental circumstances, enabling it to reach a state that it perceives as positive, i.e., that evokes positive emotions. This paper describes the role of internal as well as external factors in influencing welfare, each of which exerts their effects in a sporadic or lasting manner. Behavior is highlighted as a crucial read-out parameter. As most animals under human care are selected for certain traits that may affect their behavioral repertoire it is crucial to have thorough ethograms, i.e., a catalogue of specific behaviors of the species/strain/breed under study. DAWCon highlights aspects that need to be addressed when assessing welfare and may stimulate future research questions.

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“…Genome editing is a powerful tool to inactivate single genes in cells and animals [142]. The situation with PERV is more complicated, as it is integrated 50-70 times in the genome of a cell.…”

Section: Genome Editingmentioning

confidence: 99%

Porcine Endogenous Retroviruses and Xenotransplantation, 2021

Denner

2021

Viruses

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Porcine endogenous retroviruses (PERVs) are integrated in the genome of all pigs, and some of them are able to infect human cells. Therefore, PERVs pose a risk for xenotransplantation, the transplantation of pig cells, tissues, or organ to humans in order to alleviate the shortage of human donor organs. Up to 2021, a huge body of knowledge about PERVs has been accumulated regarding their biology, including replication, recombination, origin, host range, and immunosuppressive properties. Until now, no PERV transmission has been observed in clinical trials transplanting pig islet cells into diabetic humans, in preclinical trials transplanting pig cells and organs into nonhuman primates with remarkable long survival times of the transplant, and in infection experiments with several animal species. Nevertheless, in order to prevent virus transmission to the recipient, numerous strategies have been developed, including selection of PERV-C-free animals, RNA interference, antiviral drugs, vaccination, and genome editing. Furthermore, at present there are no more experimental approaches to evaluate the full risk until we move to the clinic.

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Current status of the application of gene editing in pigs

Cited by 20 publications

References 151 publications

The Bibliometric Landscape of Gene Editing Innovation and Regulation in the Worldwide

The Bibliometric Landscape of Gene Editing Innovation and Regulation in the Worldwide

A dynamic concept of animal welfare: The role of appetitive and adverse internal and external factors and the animal’s ability to adapt to them

Porcine Endogenous Retroviruses and Xenotransplantation, 2021

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