Current status of spent embryo media research for preimplantation genetic testing

Belandres, Denice; Shamonki, M.; Arrach, Nabil

doi:10.1007/s10815-019-01437-6

Cited by 22 publications

(12 citation statements)

References 54 publications

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“…Although some miRs such as hsa-miR-19b-3p and hsa-miR-372 have been identified in at least two independent studies (Table 1), the comparison of secreted miRs in BCM with different implantation outcomes has demonstrated a generally inconsistent result among different studies (McCallie et al, 2010;Rosenbluth et al, 2014;Cuman et al, 2015;Borges et al, 2016;Capalbo et al, 2016;Abu-Halima et al, 2020). Contributing factors to this inconsistency include diverse embryo culture conditions in different IVF clinics, unstandardized protocols, manual effects on RNA isolation, and miR detection (Belandres et al, 2019). In addition, an obvious confounder of associating miRs in BCM with failed implantation outcome is the potential effects of the endometrium.…”

Section: Spent Blastocyst Culture Mediummentioning

confidence: 99%

Secreted MicroRNA to Predict Embryo Implantation Outcome: From Research to Clinical Diagnostic Application

Zhou

Dimitriadis

2020

Front. Cell Dev. Biol.

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Embryo implantation failure is considered a leading cause of infertility and a significant bottleneck for in vitro fertilization (IVF) treatment. Confirmed factors that lead to implantation failure involve unhealthy embryos, unreceptive endometrium, and asynchronous development and communication between the two. The quality of embryos is further dependent on sperm parameters, oocyte quality, and early embryo development after fertilization. The extensive involvement of such different factors contributes to the variability of implantation potential across different menstrual cycles. An ideal approach to predict the implantation outcome should not compromise embryo implantation. The use of clinical material, including follicular fluid, cumulus cells, sperm, seminal exosomes, spent blastocyst culture medium, blood, and uterine fluid, that can be collected relatively non-invasively without compromising embryo implantation in a transfer cycle opens new perspectives for the diagnosis of embryo implantation potential. Compositional comparison of these samples between fertile women and women or couples with implantation failure has identified both quantitative and qualitative differences in the expression of microRNAs (miRs) that hold diagnostic potential for implantation failure. Here, we review current findings of secreted miRs that have been identified to potentially be useful in predicting implantation outcome using material that can be collected relatively non-invasively. Developing non-invasive biomarkers of implantation potential would have a major impact on implantation failure and infertility.

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Section: Spent Blastocyst Culture Mediummentioning

confidence: 99%

Secreted MicroRNA to Predict Embryo Implantation Outcome: From Research to Clinical Diagnostic Application

Zhou

Dimitriadis

2020

Front. Cell Dev. Biol.

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“…Attempts to use cfeDNA for non-invasive preimplantation aneuploidy testing bring to light several factors that could potentially affect the accuracy of this approach reducing concordance rates with TE biopsy results. These include maternal contamination by cumulus and corona cells 33 , cfeDNA degradation, low amounts of cfeDNA, variable DNA amplification efficacy and yield 7,36,37 , and short DNA fragments 17 .…”

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confidence: 99%

Minimally Invasive Cell-Free Human Embryo Aneuploidy Testing (miPGT-A) Utilizing Combined Spent Embryo Culture Medium and Blastocoel Fluid –Towards Development of a Clinical Assay

Kuznyetsov

Madjunkova

Abramov

et al. 2020

Sci Rep

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Preimplantation genetic testing for aneuploidies (PGT-A) using trophectoderm (TE) biopsy samples is labour intensive, invasive, and subject to sampling bias. In this study, we report on the efficacy and factors affecting accuracy of a technique we pioneered for minimally invasive preimplantation genetic testing for aneuploidy (miPGT-A). Our technique uses cell-free embryonic DNA (cfeDNA) in spent embryo culture medium (SEM) combined with blastocoel fluid (BF) to increase the amount of assayable cfeDNA. We compared miPGT-A results (n = 145 embryos) with standard PGT-A analysis of the corresponding trophectoderm biopsy. We found that accuracy of miPGT was not related to blastocyst morphological grade. The overall concordance rate per sample for euploidy/aneuploidy status between miPGT-A and TE biopsy samples was 88/90 (97.8%), and was not different between good 47/48 (97.9%) and moderate/low quality blastocysts 41/42 (97.9%) (p > 0.05). Importantly, we also discovered that for cfeDNA analysis, the SurePlex whole genome amplification (WGA) kit can be utilized without an additional cell lysis/extraction DNA step; this efficiency likely reduces the risk of maternal contamination. Regarding origin of embryonic cfeDNA, the average amount of miPGT-A WGA-DNA we obtained from blastocysts with different morphological grades, as well as the size miPGT-A WGA-DNA fragments, suggest that it is unlikely that apoptosis and necrosis are only mechanisms of DNA release from the inner cell mass (ICM) and TE into BF and SEM.

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“…However, considering its invasiveness, clinicians are looking for a more conservative technology to select euploid blastocysts. Recently, a growing number of studies has focused on the embryo medium analysis, a noninvasive alternative to the current paradigm of blastocyst biopsy (Belandres, Shamonki, & Arrach, ). Peptide analysis of the human culture medium may also hold potential applicability in IVF practice.…”

Section: Discussionmentioning

confidence: 99%

Peptidomic analysis of blastocyst culture medium and the effect of peptide derived from blastocyst culture medium on blastocyst formation and viability

Shi

Wang

et al. 2019

Molecular Reproduction Devel

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High-quality in vitro human embryo culture medium can improve the blastocyst formation rate and blastocyst quality and be beneficial for the clinical application of single blastocyst transfer. Mammalian embryos can secrete protein products into the surrounding medium. As a group of bioactive molecules and degraded proteins, peptides have been shown to participate in various biological processes.Using liquid chromatography-tandem mass spectrometry, we performed comparative peptidomic analysis of human culture medium in blastocyst formation and nonblastocyst-formation groups. A total of 201 differentially expressed peptides originating from 157 precursor proteins were identified. Among these, a peptide derived from HERC2 (peptide derived from blastocyst culture medium[PDBCM]) passed through the zona pellucida, was distributed on the perivitelline space, was absent in arrest embryos and highly expressed in high-quality blastocysts compared with low-quality blastocysts, and significantly promoted blastocyst formation in a concentration-dependent manner. These results indicate that PDBCM may be a novel biomarker for predicting blastocyst formation and viability. The mechanism remains unclear and needs to be explored in the future. K E Y W O R D Sbiomarker, blastocyst culture medium, blastocyst formation, peptide derived from blastocyst culture medium, peptidomic analysis *Ji and Shi contributed equally to this study.

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Current status of spent embryo media research for preimplantation genetic testing

Abstract: In recent years, a growing body of literature has emerged investigating the clinical utility of spent embryo media (SEM) for preimplantation genetic testing for aneuploidy (PGT-A) (Hammond et al.

Cited by 22 publications

References 54 publications

Secreted MicroRNA to Predict Embryo Implantation Outcome: From Research to Clinical Diagnostic Application

Secreted MicroRNA to Predict Embryo Implantation Outcome: From Research to Clinical Diagnostic Application

Minimally Invasive Cell-Free Human Embryo Aneuploidy Testing (miPGT-A) Utilizing Combined Spent Embryo Culture Medium and Blastocoel Fluid –Towards Development of a Clinical Assay

Peptidomic analysis of blastocyst culture medium and the effect of peptide derived from blastocyst culture medium on blastocyst formation and viability

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