Current status of multiple antigen-presenting peptide vaccine systems: Application of organic and inorganic nanoparticles

Fujita, Yoshio; Taguchi, Hiroaki

doi:10.1186/1752-153x-5-48

Cited by 69 publications

(42 citation statements)

References 120 publications

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“…They are also expected to surpass vaccines produced with attenuated or genetically-modified bacteria [12]–[13]. Synthetic immunogenic peptides are desirable components for subunit vaccines because unlike traditional vaccines they a) carry no infectious material, b) have no cross-reactivity with host tissues, c) can induce site-specific antibodies, d) are chemically defined and can be modified, and e) allow for swift large-scale manufacturing and long-term storage in the event of a pandemic [34].…”

Section: Discussionmentioning

confidence: 99%

Shigella flexneri 3a Outer Membrane Protein C Epitope Is Recognized by Human Umbilical Cord Sera and Associated with Protective Activity

et al. 2013

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Shigella flexneri 3a is one of the five major strains of the Shigella genus responsible for dysentery, especially among children, in regions of high poverty and poor sanitation. The outer membrane proteins (OMP) of this bacterium elicit immunological responses and are considered a prime target for vaccine development. When injected into mice they elicit a protective immunological response against a lethal dose of the pathogen. The OMPs from S. flexneri 3a were isolated and resolved by two-dimension-SDS-PAGE. Two 38-kDa spots were of particular interest since in our earlier studies OMPs of such molecular mass were found to interact with umbilical cord sera. These two spots were identified as OmpC by ESI-MS/MS spectrometry. By DNA sequencing, the ompC gene from S. flexneri 3a was identical to ompC from S. flexneri 2a [Gene Bank: 24113600]. A 3D model of OmpC was built and used to predict B-cell type (discontinuous) antigenic epitopes. Six epitopes bearing the highest score were selected and the corresponding peptides were synthesized. Only the peptides representing loop V of OmpC reacted strongly with the umbilical cord serum immunoglobulins. To determine which amino acids are essential for the antigenic activity of the epitope, the loop V was scanned with a series of dodecapeptides. The peptide RYDERY was identified as a minimal sequence for the loop V epitope. Truncation at either the C- or N-terminus rendered this peptide inactive. Apart from C-terminal tyrosine, substitution of each of the remaining five amino acids with glycine, led to a precipitous loss of immunological activity. This peptide may serve as a ligand in affinity chromatography of OmpC-specific antibodies and as a component of a vaccine designed to boost human immune defenses against enterobacterial infections.

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Section: Discussionmentioning

confidence: 99%

Shigella flexneri 3a Outer Membrane Protein C Epitope Is Recognized by Human Umbilical Cord Sera and Associated with Protective Activity

et al. 2013

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“…The current field is still developing but there are reports on the use of gold nanoparticles in vaccine delivery in some diseases due to their small size and ability to enter the cell easily. Fujita and Taguchi (2011) proposed that gold nanoparticle-based vaccines can be developed by two ways; the first one includes addition of functional components (T cell epitopes, cell-penetrating peptides and lipophilic moieties) and the second one is known as the synthetic approach, which is achieved by using size-defined nanomaterials (self-assembling peptides, non-peptidic dendrimers and gold nanoparticles) as antigen-displaying platforms.…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research

et al. 2015

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Nanotechnology is an emerging cutting-edge technology, which involves interdisciplinary subjects, such as physics, chemistry, biology, material science and medicine. Different methods for the synthesis of nanoparticles have been discussed here. Although physical and chemical methods have been successfully used to synthesize nanoparticles, the use of hazardous chemicals and synthesis at high temperature is a matter of concern. Hence, there is a necessity to develop eco-friendly techniques for the synthesis of nanoparticles. Biosynthesis of nanoparticles by fungi, bacteria, actinomycetes, lichen and viruses have been reported eco-friendly. Moreover, the fungal system has emerged as an efficient system for nanoparticle synthesis as fungi possess distinctive characters including high wall binding capacity, easy to culture and simpler biomass handling, etc. In this review, we have discussed fungi as an important tool for the fabrication of nanoparticles. In addition, methods and mechanism for synthesis of nanoparticles and its potential applications have also been discussed.

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“…Multiple antigenic peptide (MAP) dendrimer system is being used for vaccine and immunization purposes. MAP-based delivery can prepare by addition of multiple immune-functional components, like B/T-cell epitopes, cell-penetrating peptides, and lipophilic moieties or by controlled synthesis of nanomaterials like micelles, dendrimers, and nanoparticles (Fujita and Taguchi 2011). A tetravalent multiple antigen peptide (MAP) dendrimer with four identical branches of a C-terminal peptide sequence of the rat GH-BP (GH-BP263-279) was synthesized and used as an immunogen in rabbits.…”

Section: Dendrimermentioning

confidence: 99%

Nanotechnological Approaches for Genetic Immunization

2013

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Genetic immunization is one of the important findings that provide multifaceted immunological response against infectious diseases. With the advent of r-DNA technology, it is possible to construct vector with immunologically active genes against specific pathogens. Nevertheless, site-specific delivery of constructed genetic material is an important contributory factor for eliciting specific cellular and humoral immune response. Nanotechnology has demonstrated immense potential for the site-specific delivery of biomolecules. Several polymeric and lipidic nanocarriers have been utilized for the delivery of genetic materials. These systems seem to have better compatibility, low toxicity, economical and capable to delivering biomolecules to intracellular site for the better expression of desired antigens. Further, surface engineering of nanocarriers and targeting approaches have an ability to offer better presentation of antigenic material to immunological cells. This chapter gives an overview of existing and emerging nanotechnological approaches for the delivery of genetic materials.

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Current status of multiple antigen-presenting peptide vaccine systems: Application of organic and inorganic nanoparticles

Cited by 69 publications

References 120 publications

Shigella flexneri 3a Outer Membrane Protein C Epitope Is Recognized by Human Umbilical Cord Sera and Associated with Protective Activity

Shigella flexneri 3a Outer Membrane Protein C Epitope Is Recognized by Human Umbilical Cord Sera and Associated with Protective Activity

Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research

Nanotechnological Approaches for Genetic Immunization

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