Current status of IVM/IVF and embryo culture in humans and farm animals

Trounson, Alan; Pushett, David; Maclellan, L.J.; Lewis, Ian M.; Gardner, David K.

doi:10.1016/s0093-691x(05)80049-4

Cited by 93 publications

(28 citation statements)

References 33 publications

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“…Thus the bovine embryo is a more appropriate model than the mouse for research in fertilization and assisted-reproduction technology. Furthermore, large numbers of oocytes are easily recovered from ovaries obtained from abattoirs, which can be matured and fertilized in vitro and easily grown to the blastocyst stage in the laboratory (Trounson et al 1994). These oocytes and embryos are ideal for micromanipulation, multiplication, and genetic engineering, including cloning.…”

Section: Resultsmentioning

confidence: 99%

Centriolar dynamics in the bovine embryo: inheritance and perpetuation of the sperm centrosome during fertilization and development

et al. 1999

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Summary.Inheritance of the centrosome (centriole) and its behaviour during fertilization and embryogenesis of cattle is presented. The bovine embryo follows the human pattern of centriole behaviour, which is common to most animals including large mammals. Thus, most mammals obey Boveri's rule of paternal centrosomal inheritance and perpetuation, whereas the mouse is an exception to the rule, showing maternal inheritance. The sperm centrosome was traced from fertilization to the hatching blastocyst stage in the cow and its presence was confirmed at every stage of cleavage, as reported in the human. It is concluded that the bovine embryo is a more appropriate model than the mouse for research in fertilization and assisted-reproduction technology.

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Section: Resultsmentioning

confidence: 99%

Centriolar dynamics in the bovine embryo: inheritance and perpetuation of the sperm centrosome during fertilization and development

et al. 1999

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“…Oocyte maturation in our laboratory consistently produces approximately 70% polar body extrusion; however, not all oocytes are suitable for use in enucleation experiments on the basis of their morphological characteristics [20]. The use of in vitro-matured oocytes for nuclear transfer experiments [21-231 has shown that they have a relatively low developmental potential.…”

Section: Discussionmentioning

confidence: 99%

Enucleation by Centrifugation of in Vitro-Matured Bovine Oocytes for Use in Nuclear Transfer1

Tatham

Dowsing

Trounson

1995

Biology of Reproduction

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Nuclear transfer has the potential to produce large numbers of identical progeny. Current limitations of the technique are associated with the use of micromanipulation for the demanding enucleation and reconstitution procedure. With the overcoming of this limitation, increased numbers of nuclear transfer embryos could be produced. Centrifugation of bovine oocytes at 15,000 x g for 2 min resulted in the stratification of organelles within the cytoplasm which positioned the metaphase II spindle for enucleation. After removal of the zona pellucida with Pronase, the oocytes were centrifuged in a Percoll density gradient so that the oocytes were stretched apart to form cytoplasts and the metaphase II spindle was separated from the majority of oocytes. Enucleation by centrifugation efficiently produced a consistent population of enucleated cytoplasts from bovine in vitro-matured oocytes. The population of enucleated cytoplasts was enriched by exclusion of the cytoplasts that exhibited an extrusion cone containing metaphase II chromosomes 6 h after centrifugation. The enucleated oocyte cytoplasts were aggregated with blastomeres isolated from in vivo-collected morulae. The aggregated embryonic cells were electrofused to obtain nuclear transfer embryos that were placed into a sodium alginate false zona and were capable of cleavage and development in vitro. The development of nuclear transfer embryos produced through use of centrifugation and aggregation techniques was comparable with that of nuclear transfer embryos produced by micromanipulation techniques.

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“…Embryos were recovered by laparotomy. Each horn of the uterus was flushed by the methods of Tervit et al [10] with 20 to 30 ml of phosphate-buffered saline supplemented with 0.3% bovine serum albumin (BSA) and amino acids [11].…”

Section: Embryo Recoverymentioning

confidence: 99%

Effect of Single or Multiple Injection of Follicle Stimulating Hormone Combined with Pregnant Mare Serum Gonadotropin on Superovulatory Response, and Normal and Freezable Embryos in Ewes.

Yamada

Kawana

Tamura

et al. 1996

Journal of Reproduction and Development

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Current status of IVM/IVF and embryo culture in humans and farm animals

Cited by 93 publications

References 33 publications

Centriolar dynamics in the bovine embryo: inheritance and perpetuation of the sperm centrosome during fertilization and development

Centriolar dynamics in the bovine embryo: inheritance and perpetuation of the sperm centrosome during fertilization and development

Enucleation by Centrifugation of in Vitro-Matured Bovine Oocytes for Use in Nuclear Transfer1

Effect of Single or Multiple Injection of Follicle Stimulating Hormone Combined with Pregnant Mare Serum Gonadotropin on Superovulatory Response, and Normal and Freezable Embryos in Ewes.

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