Current status of electron spin resonance (ESR) for<i>in vivo</i>detection of free radicals

Quaresima, Valentina; Ferrari, Marco

doi:10.1088/0031-9155/43/7/015

Cited by 19 publications

(22 citation statements)

References 74 publications

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“…74 Both reactive oxygen species and NO have been detected in vivo with ESR. 75,76 Although instability, tissue metabolism, the sometimes broad reactivity of spin traps, and the cost of ESR spectrometers can be problematic, when combined with parallel strategies for detecting specific reactive species, ESR has proven to be a useful and revealing free radical detection strategy. [77][78][79] Lucigenin and LumH 2 and the light they can produce have long been studied in the hope that understanding these chemiluminescences would lead to insight into the mechanism of bioluminescence.…”

Section: Electron Spin Resonance and Spin Trappingmentioning

confidence: 99%

Methods of Detection of Vascular Reactive Species

Tarpey

Fridovich²

2001

Circulation Research

481

106

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Abstract-The evanescent nature of reactive oxygen and nitrogen species, the multiple cellular mechanisms evolved to maintain these substances at low (submicromolar) concentrations within the vascular system, and the often multifaceted nature of their reactivities have made measurement of these compounds within the vasculature problematic. This review attempts to provide a critical description of some of the most common approaches to quantification of nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite, with attention to key issues that may influence the utility of a particular assay when adapted for use in vascular cells and tissues.

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Section: Electron Spin Resonance and Spin Trappingmentioning

confidence: 99%

Methods of Detection of Vascular Reactive Species

Tarpey

Fridovich²

2001

Circulation Research

481

106

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“…Several review articles on the development of EPRI methods and their application to biological systems have appeared in recent years [3,[13][14][15]. While the time-domain EPRI technique has emerged recently with the advantage of significantly reduced imaging time [16,17], the continuous wave (CW) EPRI technique still dominates current applications because of its higher sensitivity and applicability to a large variety of spin probes of varied linewidths [17].…”

Section: Introductionmentioning

confidence: 99%

Progressive EPR imaging with adaptive projection acquisition

Deng

Kuppusamy

Zweíer

2005

Journal of Magnetic Resonance

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Continuous wave electron paramagnetic resonance imaging (EPRI) of living biological systems requires rapid acquisition and visualization of free radical images. In the commonly used multiplestage back-projection image reconstruction algorithm, the EPR image cannot be reconstructed until a complete set of projections is collected. If the data acquisition is incomplete, the previously acquired incomplete data set is no longer useful. In this work, a 3-dimensional progressive EPRI technique was implemented based on inverse Radon transform in which a 3-dimensional EPR image is acquired and reconstructed gradually from low resolution to high resolution. An adaptive data acquisition strategy is proposed to determine the significance of projections and acquire them in an order from the most significant to the least significant. The image acquisition can be terminated at any time if further collection of projections does not improve the image resolution distinctly, providing flexibility to trade image quality with imaging time. The progressive imaging technique was validated using computer simulations as well as imaging experiments. The adaptive acquisition uses 50-70% less projections as compared to the regular acquisition. In conclusion, adaptive data acquisition with progressive image reconstruction should be very useful for the accelerated acquisition and visualization of free radical distribution.

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“…EPR spectroscopy, a magnetic resonance technique similar to NMR spectroscopy, is specific for the detection of paramagnetic species such as free radicals. However, the very low levels of endogenous free radicals (Ͻ nM), their extremely short half-lives (Ͻ ns), and relatively short spinspin and spin-lattice relaxation times (Ͻ s) have made the implementation of EPR imaging (EPRI) modalities challenging in intact biological objects (4,5). However, using exogenous spin probes and continuous wave (CW) mode of data acquisition, EPR spectroscopy and imaging techniques have provided spatially encoded physiologic information such as tissue redox status and pO 2 levels (6 -11).…”

Section: Introductionmentioning

confidence: 99%