Current status of biology, bioengineering, and therapeutic potential of stem cells

Cha, Hyuk‐Jin; Hwang, Eun Sook

doi:10.1007/s12272-012-0200-1

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…However, the recent study showed that even patient´s own iPSCs may induce immune rejection. Moreover, when viral vectors are used to engineer the expression vectors for the various methods for genetic repair, immune rejection may be developed towards the viral vector (Cha and Hwang, 2012;Pawitan, 2012;Stadtfeld and Hochedlinger, 2010;Yamanaka, 2009).…”

Section: Aberrant Reprogramming May Results In An Impairedmentioning

confidence: 99%

Induced Pluripotent Stem Cells and Their Implication for Biomedical Sciences

Csobonyeiová¹

2013

OnLine Journal of Biological Sciences

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The groundbreaking discovery, that somatic mammalian cells can be epigenetically reprogrammed to a pluripotent state through the exogenous expression of the transcription factors Oct4, Sox2, Klf4 and c-myc, has yielded a new cell type for potential application in regenerative medicine, the induced Pluripotent Stem Cells (iPSCs). Since the first demonstration of creating iPSCs in 2006 great efforts have been made into improving iPS cell generation methods and understanding the reprogramming mechanism as well as the nature of iPSCs. The iPSCs technique makes it possible to produce patient-specific pluripotent stem cells for transplantation therapy without immune rejection. However, some restriction still remain, including viral vector integration into the genome, the existence of exogenous oncogenic factors and low induction efficiency. In this review we discuss recent advances in methods of generating safer iPSCs lines and their possible use for biomedical applications.

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Section: Aberrant Reprogramming May Results In An Impairedmentioning

confidence: 99%

Induced Pluripotent Stem Cells and Their Implication for Biomedical Sciences

Csobonyeiová¹

2013

OnLine Journal of Biological Sciences

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“…In laboratory model experiments, mesenchymal stem cells have been co-cultured with scaffolds. Producing functional parts of the respiratory system, provided evidence that these cells might be used for tissue repair in the airways [ 44 ]. The same observation has been made for other diseases of the respiratory system such pulmonary fibrosis, cystic fibrosis and pulmonary hypertension [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

A gene therapy induced emphysema model and the protective role of stem cells

et al. 2014

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BackgroundChronic obstructive pulmonary disease presents with two different phenotypes: chronic bronchitis and emphysema with parenchymal destruction. Decreased expression of vascular endothelial growth factor and increased endothelial cell apoptosis are considered major factors for emphysema. Stem cells have the ability of vascular regeneration and function as a repair mechanism for the damaged endothelial cells. Currently, minimally invasive interventional procedures such as placement of valves, bio-foam or coils are performed in order to improve the disturbed mechanical function in emphysema patients. However, these procedures cannot restore functional lung tissue. Additionally stem cell instillation into the parenchyma has been used in clinical studies aiming to improve overall respiratory function and quality of life.MethodsIn our current experiment we induced emphysema with a DDMC non-viral vector in BALBC mice and simultaneously instilled stem cells testing the hyposthesis that they might have a protective role against the development of emphysema. The mice were divided into four groups: a) control, b) 50.000 cells, c) 75.000 and d) 100.000 cells.ResultsLung pathological findings revealed that all treatment groups had less damage compared to the control group. Additionally, we observed that emphysema lesions were less around vessels in an area of 10 μm.ConclusionsOur findings indicate that stem cell instillation can have a regenerative role if applied upon a tissue scaffold with vessel around.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_195

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Source-Cell Storage Systems for Allogeneic Cell Therapy Products: Comparison of the Regulatory Systems in Target Countries

Khan

Shin

Kim

2023

Research in Clinical Pharmacy

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Background: This study aimed to analyze autologous and allogeneic cell therapies. A gap analysis of source-cell storage regulations and guidelines of allogeneic cell therapies was performed for regulatory authorities in target countries, including the USA, EU, Japan, and Korea.Methods: Cell-storage regulations and guidelines regarding allogeneic cell therapy products framed by regulatory authorities were comparatively analyzed.Results: Of 41 cell therapies, 10 allogeneic cell therapies were selected, and their source cells were categorized based on 3 types of cell storage, namely cell bank (n=6) in the USA, Japan, and Korea; cell stock (n=2) in the EU; and drug substance (n=2) in Korea. In the gap analysis of cell storage regulations and guidelines by the selected regulatory authorities, cell storage systems have regulations and guidelines similar to those stipulated by regulatory authorities, except for the regulation of source-cell changes. Most allogeneic cell therapies used cell banks for cell storage. Thus, to ensure the safety of allogeneic cell therapy, more detailed criteria must be established for source-cell changes in cell storage systems. Conclusion:Regulatory authorities can ensure a safer and more effective supply of therapy products to the public by harmonizing the regulations associated with allogeneic cell therapies.

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Current status of biology, bioengineering, and therapeutic potential of stem cells

Cited by 3 publications

References 41 publications

Induced Pluripotent Stem Cells and Their Implication for Biomedical Sciences

Induced Pluripotent Stem Cells and Their Implication for Biomedical Sciences

A gene therapy induced emphysema model and the protective role of stem cells

Source-Cell Storage Systems for Allogeneic Cell Therapy Products: Comparison of the Regulatory Systems in Target Countries

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