Current Status of Binary Vectors and Superbinary Vectors

Delivery of Multiple Transgenes to Plant Cells

“…McCormac et al, 1997;Curtis and Grossniklaus, 2003;Bracha-Drori et al, 2004;Earley et al, 2006;Coutu et al, 2007) provide the plant research community with a vast number of versatile tools for various plant expression analyses. (For a recent update on binary vectors, the reader is referred to an update by Komori et al, 2007).…”

“…An et al, 1985;Simoens et al, 1986;Becker et al, 1992). The constant improvements in binary vectors even included the most famous binary vector, one which has been dominating the landscape of binary plasmids for several decades: pBin19 (Komori et al, 2007). This plasmid, the first to be commercialized for use in plant genetic transformation, to our knowledge, offers several features, including incorporation of the LacZ gene into the MCS to facilitate identification of recombinant plasmids using a colorimetric assay, a bacterial kanamycin-resistance gene, an E. coli ori, a complete plant selection marker expression cassette, and an extended MCS.…”

Review: Plant Binary Vectors of Ti Plasmid in <i>Agrobacterium tumefaciens</i> with a Broad Host-Range Replicon of pRK2, pRi, pSa or pVS1

“…A binary plant vector strategy was designed to separate the T-DNA region in a small plasmid from the virulence genes in avirulent T-DNA-less Ti plasmid [9]. The small plant vectors with the T-DNA region have been simply now called binary Ti vectors [10,11]. [12].…”

Advanced Expression Vector Systems: New Weapons for Plant Research and Biotechnology

“…Thus, for example, the progress in plant genetic engineering could not have been as productive as it is today without the development of small, easy-to-manipulate, and simple-to-use Agrobacterium binary vectors (e.g. Komari et al, 2006;Komori et al, 2007), and studies of protein subcellular localization in plant cells have been greatly simplified and advanced with the introduction of vectors that express GFP fusions (Goodin et al, 2007). Indeed, the ability to transiently and stably express foreign genes, to genetically interfere with the expression of native genes, and to functionally study the expression, interaction, localization, and modification of proteins in cells, tissues, and whole plants are fundamental to modern plant basic research and biotechnology.…”