Current status of bacterial contamination in some fish species of Bakkhali river Estuary, Bangladesh

Jahan, Sarwar; Jewel, Md. Abu Sayed; Haque, Md. Anamul; Hasan, Jahid; Mita, M.M.; Aktar, A.; Al-Amin, Md.

doi:10.26832/24566632.2019.0401015

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Okonkwo et al [23] did not isolate any Vibrio species from crab samples in their findings; a result which supported absolutely with the result gotten from this findings. Vibrio cholerae reported by Jahan et al [24] supported the findings from this study as two V. cholerae were identified from fish samples. No Vibrio species was isolated from shrimp samples in their findings, an outcome which was parallel to Okonkwo et al [23].…”

Section: Discussionsupporting

confidence: 91%

Antibiotic Sensitivity and Plasmid Assessment of Ampicillin-Resistant Vibrio cholerae from Seafoods in Coastal Areas of Ondo State

Onifade

Adeyanju

2022

Microbes and Infectious Diseases

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Background: Vibrio cholerae is an epitome of non-invasive organisms, which only affects the small intestine via the liberation of enterotoxin. The plasmid profile and beta lactamase production in ampicillin-resistant Vibrio species isolated from seafoods collected from coastal area of Ondo State, Nigeria were investigated. Methods: One hundred and six (106) samples of seafoods were collected in February, 2020 and January, 2021. The isolation and identification of Vibrio species, antibiotic sensitivity pattern, plasmid analysis and beta-lactamase detection activity were conducted via pour plate, Kirby-Bauer disc diffusion, alkaline lysis, neutralization, electrophoresis methods, use of 10 % sodium dodecyl sulphate (SDS) and double disk synergistic test (DDST) respectively. Results: Pathogenic bacteria such as Bacillus subtilis, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, and Vibrio cholerae were isolated from 10 crabs, 54 fishes, and 42 shrimps. Findings also revealed that 66% of Vibrio cholerae that showed multiple antibiotic resistance tested positive to beta lactamase production test. The result of plasmid analysis indicated that they were more susceptible to antibiotics after plasmid curing of the microorganisms. Conclusion: The resistance of Vibrio cholerae in this investigation was found to be plasmid-mediated consequent to its loss after curing. The detection of multidrug resistance, ESBLs-producing Vibrio organisms, suggests risks to public health. The risk of seafood-borne infections can be reduced by decrease in pollution levels, ensuring appropriate cooking temperatures, and prevention of crosscontamination during harvesting, handling and processing.

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Section: Discussionsupporting

confidence: 91%

Antibiotic Sensitivity and Plasmid Assessment of Ampicillin-Resistant Vibrio cholerae from Seafoods in Coastal Areas of Ondo State

Onifade

Adeyanju

2022

Microbes and Infectious Diseases

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“…The presence of S. aureus and CNS means that the fish were contaminated from human and animal wastes at the source (lake) or during handling. In this study, there was no Salmonella , Listeria , Vibrio , and Shigella genera, which were isolated by [3, 4, 16, 20]. In our study, we isolated more bacteria genera (8) than that in [3, 4, 16, 20].…”

Section: Discussionmentioning

confidence: 52%

“…There is also a problem of bursting of sewage pipes, thus exuding raw untreated sewage from the high-density suburbs. Fish are contaminated from the sewage and industrial wastes [20] as well as from poor handling techniques by vendors.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Profiling of Bacteria Isolated from Fish Sold at Informal Market in Mufakose, Zimbabwe

Gufe¹,

Hodobo²,

Mbonjani³

et al. 2019

International Journal of Microbiology

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The number of infections caused by antibiotic resistant bacteria is rising worldwide. Fish from multisource pollution waters can harbour multidrug-resistant bacteria that can be disseminated to humans through eating or contact of contaminated fish. A cross-sectional study was carried out to (i) isolate and phenotypically identify bacteria from 36 fish samples from informal market in Mufakose, Harare, and (ii) determine the antibiotic sensitivity pattern of the isolated bacteria against ten available antibiotics (ampicillin 10 μg, gentamycin 30 μg, penicillin G 10 μg, erythromycin 15 μg, tetracycline 30 μg, kanamycin 30 μg, neomycin 10 μg, cloxacillin 5 μg, lincomycin 15 μg, and sulfamethoxazole 25 μg) using the Kirby–Bauer disk agar diffusion method. Eight bacterial genera were isolated and identified, and they were Escherichia, Aeromonas, Staphylococcus, Pseudomonas, Citrobacter, Klebsiella, Enterobacter, and Proteus. Among the isolates, Escherichia coli was isolated most frequently (44%) followed by Staphylococcus aureus (19%), Enterobacter aerogenes (7%), Aeromonas spp. (5%), Proteus mirabilis (5%), Citrobacter (5%), and coagulase-negative Staphylococci (5%) and the least frequent were Klebsiella (3%) and Pseudomonas (3%). All isolates were susceptible to gentamycin. Varying antibiotic resistance rates were observed to lincomycin (100%), ampicillin (81%), penicillin (67%), erythromycin (65%), tetracycline (63%), neomycin (61%), cloxacillin (43%), kanamycin (24%), and sulphamethoxazole (13%). All the isolates were multidrug-resistant (resistant to at 3 or more drugs tested) except Proteus mirabilis. Proteus mirabilis has multiple antibiotic resistance (MAR) index of 0.2, and the other isolated bacteria had MAR indexes greater than 0.2 ranging from 0.3 to 0.7. Those MAR indexes above 0.2 showed that the bacteria isolates are from a high risk source where antibiotics were frequently used, possibly from sewage effluents. Isolation of enteric bacteria such as Escherichia coli is an indication of faecal contamination, and this poses a high risk to animal and human health. These significant findings call for effective risk assessment models and management plans that protect human, animal, and environmental health.

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“…A study of the bacterial count on the skin surface of Etroplus suratensis collected from upstream and downstream regions of the Mogral river in Kasargod, Kerala was 4.41 and 4.92 log CFU g -1 respectively (Megha and Harikumar, 2016). Jahan et al (2019) showed that the bacterial count on the surface of the fish, which varied with the species and the source, ranged from 3.5 to 5.17 log CFU g -1 .…”

Section: Discussionmentioning

confidence: 99%

Incidence of Food borne Pathogens in Seafood Sourced from Neendakara and Thangassery Harbours in Kollam, Kerala, India

Nathan,

H. D. Cruz,

Kesiya S.

2023

EEC

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Seafood is a major foreign exchange earner and the microbiological quality of exported fish and shellfish is crucial for their safety and acceptance in international trade. However, seafood that is sold for local consumption in India undergoes very limited safety or quality checks. If fish are not harvested, processed and handled hygienically, they can harbour pathogenic microorganisms that can cause foodborne infections and intoxications and become a major health concern. The present work was carried out to determine the total viable bacterial count and to evaluate the presence of bacterial pathogens, such as Escherichia coli, Salmonella and Vibrio cholerae, on the surface of fish and prawns collected from Neendakara and Thangassery harbours in Kollam district during the monsoon season. The total viable aerobic plate count in the seafood ranged from 2.74-3.08 log CFU cm -2 . The fish collected from both the harbours as well as the prawns collected from Thangassery harbour were contaminated with E. coli. The samples of prawns collected from Neendakara were found to contain V. cholerae, a pathogen that should be absent in 25 g of seafood as per the Food and Safety Standards Authority of India (FSSAI). Salmonella was not detected in any of the samples. Crushed ice collected from the nearby ice plants did not show any evidence of the tested pathogens, suggesting that the seafood contamination may have come from other sources such as contact with surfaces, handlers or polluted water. Therefore, proper monitoring and control measures need to be implemented by relevant authorities to ensure the quality and safety of domestic seafood and prevent seafood-associated infections.

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Current status of bacterial contamination in some fish species of Bakkhali river Estuary, Bangladesh

Cited by 4 publications

References 13 publications

Antibiotic Sensitivity and Plasmid Assessment of Ampicillin-Resistant Vibrio cholerae from Seafoods in Coastal Areas of Ondo State

Antibiotic Sensitivity and Plasmid Assessment of Ampicillin-Resistant Vibrio cholerae from Seafoods in Coastal Areas of Ondo State

Antimicrobial Profiling of Bacteria Isolated from Fish Sold at Informal Market in Mufakose, Zimbabwe

Incidence of Food borne Pathogens in Seafood Sourced from Neendakara and Thangassery Harbours in Kollam, Kerala, India

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