Current Status of Automatic Serial Sections for 3D Electron Microscopy

Abstract

The automatic equipment for three-dimensional electron microscopy (3DEM) can acquire serial sections of a large sample in a relatively short time, and is especially suitable for the connectomics, which is a field related to understanding the brain structure as a whole. As many results obtained through 3DEM using automatic serial sections have been published in the field of brain research, many researchers continue to apply this technique to various samples. We reviewed the equipment for automatic serial sectioning, the block preparation method, the limitations of 3DEM, and future directions.

3D imaging of human epidermis micromorphology by combining fluorescent dye, optical clearing and confocal microscopy

“…18,19 One of 3D approach on skin epidermis was carried out in electron microscopy with serial block-face imaging. 20 This technique presents the advantage of very high resolution but only on small skin volume.…”