Current status in resistance to ACCase and ALS-inhibiting herbicides in rigid ryegrass populations from cereal crops in North of Tunisia

Khammassi, Messaad; Hajri, Haifa; Menchari, Yosra; Chaâbane, Hanène; Souissi, Touraya

doi:10.1017/s002185962000009x

Cited by 10 publications

(11 citation statements)

References 28 publications

(47 reference statements)

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“…According to this study, putative populations resistant to ALS inhibitors could be widely distributed in Northern Tunisia. Similar results were found for D. sophia in China [ 34 ], as well for L. rigidum in Tunisia [ 40 ], another troublesome weed species in the region, which behaved similarly and had evolved resistance in 87% of tested populations [ 41 ]. At field rate, at least for one of the two sulfonylureas tested, the dry weight increased compared to nontreated plants in most populations which suggest a hormetic effect induced in G. coronaria resistant plants.…”

Section: Discussionsupporting

confidence: 75%

Field Survey and Resistance Occurrence to ALS-Inhibiting Herbicides in Glebionis coronaria L. in Tunisian Wheat Crops

Hada

Khammassi

Jenfaoui

et al. 2020

Plants

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Glebionis coronaria (L.) Cass. ex Spach is a troublesome weed in cereal cropping systems in northern Tunisia. Recently, failures in controlling this weed have been reported by farmers. Field surveys and farmers interviews were conducted to highlight the potential causes of G. coronaria occurrence and the associated yield losses in wheat. Survey results revealed a significant correlation between farmers’ awareness of resistance occurrence and cultural practices, mainly sowing date and tillage, while G. coronaria abundance was related to the lack of herbicide rotation and the frequency of ALS-inhibiting herbicide use. High G. coronaria infestations (more than 20 plants/m2) caused a significant decrease in wheat grain yield, reaching almost 75% at a density of 100 plants/m2. Field and pot experiments showed low efficacies of ALS-inhibiting herbicides to control G. coronaria populations. The application of field rates of tribenuron-methyl and mesosulfuron + iodosulfuron failed to control the tested populations, and generally, G. coronaria dry weight increased compared to nontreated ones (potential hormetic effect). These findings were further investigated in two selected resistant populations through tribenuron dose–response experiments, plants from both populations exhibited high resistance factors (greater than 300), surviving up to 16-fold the recommended field dose. This is the first report for G. coronaria resistance occurrence to ALS-inhibiting herbicides in Tunisia and the second case worldwide.

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Section: Discussionsupporting

confidence: 75%

Field Survey and Resistance Occurrence to ALS-Inhibiting Herbicides in Glebionis coronaria L. in Tunisian Wheat Crops

Hada

Khammassi

Jenfaoui

et al. 2020

Plants

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“…Results from this study revealed the development of cross-resistance to ALS-inhibiting herbicides in two G. coronaria populations from the Bizerte region in northern Tunisia. Since their introduction in 1999, the SU herbicides were excessively used by the Tunisian wheat growers to control troublesome grass weeds such as L. rigidum ( Khammassi et al, 2020 ) and dicotyledonous weeds. The main reason was their broad-spectrum weed control at low rates.…”

Section: Discussionmentioning

confidence: 99%

Point Mutations as Main Resistance Mechanism Together With P450-Based Metabolism Confer Broad Resistance to Different ALS-Inhibiting Herbicides in Glebionis coronaria From Tunisia

et al. 2021

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Resistance to acetolactate synthase (ALS) inhibiting herbicides has recently been reported in Glebionis coronaria from wheat fields in northern Tunisia, where the weed is widespread. However, potential resistance mechanisms conferring resistance in these populations are unknown. The aim of this research was to study target-site resistance (TSR) and non-target-site resistance (NTSR) mechanisms present in two putative resistant (R) populations. Dose–response experiments, ALS enzyme activity assays, ALS gene sequencing, absorption and translocation experiments with radiolabeled herbicides, and metabolism experiments were carried out for this purpose. Whole plant trials confirmed high resistance levels to tribenuron and cross-resistance to florasulam and imazamox. ALS enzyme activity further confirmed cross-resistance to these three herbicides and also to bispyribac, but not to flucarbazone. Sequence analysis revealed the presence of amino acid substitutions in positions 197, 376, and 574 of the target enzyme. Among the NTSR mechanisms investigated, absorption or translocation did not contribute to resistance, while evidences of the presence of enhanced metabolism were provided. A pretreatment with the cytochrome P450 monooxygenase (P450) inhibitor malathion partially synergized with imazamox in post-emergence but not with tribenuron in dose–response experiments. Additionally, an imazamox hydroxyl metabolite was detected in both R populations in metabolism experiments, which disappeared with the pretreatment with malathion. This study confirms the evolution of cross-resistance to ALS inhibiting herbicides in G. coronaria from Tunisia through TSR and NTSR mechanisms. The presence of enhanced metabolism involving P450 is threatening the chemical management of this weed in Tunisian wheat fields, since it might confer cross-resistance to other sites of action.

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“…Globally, there have been numerous herbicide resistance surveys of specific cropping regions 28–33 that typically focus on single weed species at one point in time, screened with herbicides representing a limited selection of modes of action (MOAs). These surveys provide a ‘snapshot in time’ description of the extent of herbicide resistance, however, they have not typically been repeated and any subsequent changes in resistance types or distribution have not been tracked.…”

Section: Herbicide Resistance Surveysmentioning

confidence: 99%

Increasing the value and efficiency of herbicide resistance surveys

Squires

Coleman

Broster

et al. 2021

Pest Management Science

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The scale of herbicide resistance within a cropping region can be estimated and monitored using surveys of weed populations. The current approach to herbicide resistance surveys is time‐consuming, logistically challenging and costly. Here we review past and current approaches used in herbicide resistance surveys with the aims of (i) defining effective survey methodologies, (ii) highlighting opportunities for improving efficiencies through the use of new technologies and (iii) identifying the value of repeated region‐wide herbicide resistance surveys. One of the most extensively surveyed areas of the world's cropping regions is the Australian grain production region, with >2900 fields randomly surveyed in each of three surveys conducted over the past 15 years. Consequently, recommended methodologies are based on what has been learned from the Australian experience. Traditional seedling‐based herbicide screening assays remain the most reliable and widely applicable method for characterizing resistance in weed populations. The use of satellite or aerial imagery to plan collections and image analysis to rapidly quantify screening results could complement traditional resistance assays by increasing survey efficiency and sampling accuracy. Global management of herbicide‐resistant weeds would benefit from repeated and standardized surveys that track herbicide resistance evolution within and across cropping regions. © 2021 Society of Chemical Industry.

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Current status in resistance to ACCase and ALS-inhibiting herbicides in rigid ryegrass populations from cereal crops in North of Tunisia

Cited by 10 publications

References 28 publications

Field Survey and Resistance Occurrence to ALS-Inhibiting Herbicides in Glebionis coronaria L. in Tunisian Wheat Crops

Field Survey and Resistance Occurrence to ALS-Inhibiting Herbicides in Glebionis coronaria L. in Tunisian Wheat Crops

Point Mutations as Main Resistance Mechanism Together With P450-Based Metabolism Confer Broad Resistance to Different ALS-Inhibiting Herbicides in Glebionis coronaria From Tunisia

Increasing the value and efficiency of herbicide resistance surveys

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