Current Status and Challenges in Identifying Disease Resistance Genes in Brassica napus

Neik, Ting Xiang; Barbetti, Martin J.; Batley, Jacqueline

doi:10.3389/fpls.2017.01788

Cited by 95 publications

(53 citation statements)

References 375 publications

(525 reference statements)

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“…B. oleracea species encompass many popular and nutritious vegetable crops including cabbages, cauliflower, broccoli, brussels sprout, kohlrabi and kale. These species are susceptible to a range of diseases including blackleg, clubroot, sclerotinia stem rot, downy mildew and powdery mildew (Channon, ; Neik et al ., ; Punithalingham and Holliday, ; Voorrips, ).…”

Section: Introductionmentioning

confidence: 99%

Variation in abundance of predicted resistance genes in the Brassica oleracea pangenome

Bayer

Golicz

Tirnaz

et al. 2018

Plant Biotechnology Journal

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110

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Brassica oleracea is an important agricultural species encompassing many vegetable crops including cabbage, cauliflower, broccoli and kale; however, it can be susceptible to a variety of fungal diseases such as clubroot, blackleg, leaf spot and downy mildew. Resistance to these diseases is meditated by specific disease resistance genes analogs (RGAs) which are differently distributed across B. oleracea lines. The sequenced reference cultivar does not contain all B. oleracea genes due to gene presence/absence variation between individuals, which makes it necessary to search for RGA candidates in the B. oleracea pangenome. Here we present a comparative analysis of RGA candidates in the pangenome of B. oleracea. We show that the presence of RGA candidates differs between lines and suggests that in B. oleracea, SNPs and presence/absence variation drive RGA diversity using separate mechanisms. We identified 59 RGA candidates linked to Sclerotinia, clubroot, and Fusarium wilt resistance QTL, and these findings have implications for crop breeding in B. oleracea, which may also be applicable in other crops species.

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Section: Introductionmentioning

confidence: 99%

Variation in abundance of predicted resistance genes in the Brassica oleracea pangenome

Bayer

Golicz

Tirnaz

et al. 2018

Plant Biotechnology Journal

Self Cite

110

View full text Add to dashboard Cite

show abstract

“…Most R genes identified to date encode nucleotide-binding leucine-rich repeats (NB-LRRs), including coiled-coil NB-LRRs (CC-NB-LRRs) and Toll interleukin 1 receptor NB-LRRs (TIR-NB-LRRs). Moreover, some R genes encode receptor-like kinases (RLKs), transmembrane receptor-like proteins (RLPs), cytoplasmic kinases, and proteins with atypical molecular motifs [6][7][8][9] . Various R genes with flexible molecular mechanisms provide powerful weapons that protect the plant host from pathogens.…”

Section: Introductionmentioning

confidence: 99%

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

Fang

Yang

et al. 2020

Hortic Res

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Brassica species include many economically important crops that provide nutrition and health-promoting substances to humans worldwide. However, as with all crops, their production is constantly threatened by emerging viral, bacterial, and fungal diseases, whose incidence has increased in recent years. Traditional methods of control are often costly, present limited effectiveness, and cause environmental damage; instead, the ideal approach is to mine and utilize the resistance genes of the Brassica crop hosts themselves. Fortunately, the development of genomics, molecular genetics, and biological techniques enables us to rapidly discover and apply resistance (R) genes. Herein, the R genes identified in Brassica crops are summarized, including their mapping and cloning, possible molecular mechanisms, and application in resistance breeding. Future perspectives concerning how to accurately discover additional R gene resources and efficiently utilize these genes in the genomic era are also discussed.

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“…In either situation, DNA markers within the gene of interest are the ultimate due to no recombination occurrence between trait and marker [10]. In view to promote and improve disease-resistance in tomato, various markers were extensively and successfully deployed in breeding programs [11][12][13]. With strong focus on resistance breeding in tomato, resistance traits have become important characteristics in distinctness, uniformity and stability (DUS) testing for Plant Breeders Rights (PBR) applications.…”

Section: Introductionmentioning

confidence: 99%

Validation of some disease-resistance molecular markers associated with multiple diseases in tomato for marker-assisted selection program

Nevame¹,

Xia²,

Zhang³

et al. 2020

ScienceAsia

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Marker-assisted selection (MAS) is a tool that is widely applied in tomato resistance breeding. To determine the robustness of some molecular markers commonly used in MAS, extensive screening of 964 tomato lines was performed under a controlled experimental condition. Initially, the application of 36 molecular markers targeting 26 resistance genes (R genes) and 14 major diseases was evaluated. Here, we employed basic molecular biology and bioinformatics techniques for analysis where polymorphism, accuracy and clearness of amplicons constituted the selection criteria of markers. Upon initial analysis, 20 of these markers designated as efficient markers, among which 8 were considered gene-based markers and referred to as perfect markers were selected for detail evaluation. Information extrapolated from PCR result revealed 18 R genes that control 12 diseases were grouped under efficient markers. On the other hand, grouping of breeding lines based on the number of R gene harbored comprehensively revealed 62% of the lines to be void of R gene, while 38% carry different types of R genes. This provides us with an avenue to better understand new sources of resistance in the breeding lines. Conclusively, these efficient markers and their limited PCR condition can be suggested as basis of a diagnostic kit for MAS applications against 12 major tomato diseases and the identified resistant breeding lines could be conserved in order to be propagated as different sources of resistance for the development of new resistant varieties. Therefore, in areas with high vulnerability to diseases, high efficiency combination of the relevant R genes and their pyramiding into commercial tomato varieties are proposed to be implemented as a pragmatic approach.

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Current Status and Challenges in Identifying Disease Resistance Genes in Brassica napus

Cited by 95 publications

References 375 publications

Variation in abundance of predicted resistance genes in the Brassica oleracea pangenome

Variation in abundance of predicted resistance genes in the Brassica oleracea pangenome

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

Validation of some disease-resistance molecular markers associated with multiple diseases in tomato for marker-assisted selection program

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