Current status and applications of genus Geobacillus in the production of industrially important products—a review

Khaswal, Ashutosh; Chaturvedi, Neha; Mishra, Santosh Kumar; Kumar, Priya Ranjan; Paul, Prabir Kumar

doi:10.1007/s12223-022-00961-w

Cited by 20 publications

(13 citation statements)

References 111 publications

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“…The results observed here are different from those observed by Balakrishnan et al 21 who studied α‐amylase produced by Aspergillus oryzae and observed an optimum temperature of 32.5°C. The ability of a few enzymes to turn substrates into products at higher temperatures can highly improve their potential exploitation for many biotechnological applications 22 …”

Section: Resultsmentioning

confidence: 99%

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Pasin,

Betini,

de Lucas

et al. 2023

Biotechnology Progress

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Aspergillus species have been highlighted in enzyme production looking for industrial applications, notably, amylases are one of the most interesting enzymes. They are capable of hydrolyzing α‐glycosidic linkages of starch and widely used in industrial processes to produce ethanol, glucose, and fructose syrup as well as in the textiles, detergents, and paper industries applications. In this context, this work aimed at the biochemical characterization of the glucoamylase from Aspergillus japonicus and its application in the bio‐bleaching process of recycled paper. The optimum temperature and pH for the glucoamylase assay were standardized as 50°C and 5.5. After 1 h of incubation, glucoamylase retained 90% of its activity at 30–50°C. It also kept 70% of its activity in the pH range of 4.0–6.5 after an hour of incubation. The enzyme led to an increase of 30% in the relative whiteness of 10 dry grams of sulfite paper and magazine paper when applied along with commercial cellulase and 10 mM MnCl2. In addition, after the treatments, the glucoamylase recovered activity was 30%–32%, which indicates a prolonged availability of the enzyme and can considerably curtail the redundant downstream process of the recycled paper bio‐bleaching. Thus, the glucoamylase from A. japonicus has a significant role in bio‐bleaching recycled paper, reducing the necessity of hard chemicals, and improving the industrial process in an interesting economic and ecological mode.

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Section: Resultsmentioning

confidence: 99%

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Pasin,

Betini,

de Lucas

et al. 2023

Biotechnology Progress

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“…Geobacillus sp. has been reported for starch-digesting enzyme production, with growth at high temperatures without the need for cooling systems, and produced high amounts of enzyme for industrial-scale applications [28,29]. Many studies found that α-amylase from Geobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Phonlamai,

Kingkaew,

Prajanket

et al. 2024

J Basic Microbiol

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Geobacillus kaustophilus TSCCA02, a newly isolated strain from cassava (Manihot esculenta L.) rhizosphere soil in Thailand, showed maximum raw starch degrading enzyme (RSDE) activity at 252.3 ± 9.32 U/mL with cassava starch and peptone at 5.0 and 3.0 g/L, respectively. 16 S ribosomal RNA (rRNA) sequencing and phylogenetic tree analyses indicated that the TSCCA02 strain was closely related to G. kaustophilus. The crude RSDE had optimal activity at 60°C and pH 9.0. This enzyme degraded various kinds of starch including potato starch, cassava starch, rice flour, corn starch, glutinous rice flour, and wheat flour to produce sugar syrup at 60°C, as confirmed by scanning electron microscopy (SEM), thin‐layer chromatography (TLC), and Fourier‐transform infrared spectroscopy (FTIR). The major end products of starch hydrolysis were maltose and maltotriose with a small amount of glucose, confirming this enzyme as an α‐amylase. The enzyme improved the washing efficiency of cotton fabric with commercial detergent. Results indicated the potential of alkaline α‐amylase produced from a new isolate of G. kaustophilus TSCCA02 for application as a detergent additive on an industrial scale.

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“…The genus Geobacillus was documented initially by Nazina [ 74 ], and initially ten species were identified [ 75 ]. This genus is composed of thermoresistant (25–45 °C) and obligate thermophilic microorganisms (50 °C to 75 °C), proliferating in a pH range between 6.0 and 8.5, that are chemo-organotrophic, including aerobic, anaerobic, and facultative anaerobic members, classifications consistent with those observed in the characterization of the strains isolated in this study [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation of Thermophilic Bacteria from Extreme Environments in Northern Chile

Valenzuela,

Solís-Cornejo,

Araya

et al. 2024

Microorganisms

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The northern region of Chile boasts unique geographical features that support the emergence of geothermal effluents, salt lagoons, and coastal creeks. These extreme climate conditions create polyextreme habitats for microorganisms, particularly adapted to survive these harsh environments. These extremophilic microorganisms hold immense potential as a source of hydrolytic enzymes, among other biotechnological applications. In this study, we isolated 15 strains of aerobic thermophilic bacteria (45–70 °C) from sediment samples collected at five different ecological sites, including hot springs, geothermal fields, and lagoons in the Atacama Desert and Andes high planes. Analyses of the 16S rRNA gene sequences of the isolates showed a close genetic similarity (98–100%) with microorganisms of the genera Parageobacillus, Geobacillus, Anoxybacillus, and Aeribacillus. Notably, these thermophiles exhibited significant hydrolytic enzyme activity, particularly amylases, lipases, and proteases. These findings underscore the potential of using these thermophilic bacterial strains as an invaluable source of thermozymes with wide-ranging applications in diverse industries, such as detergent formulations, pharmaceutical processing, and food technology. This research highlights the ecological significance of these extreme environments in the Atacama Desert and Andes high plains, which serve as vital ecological niches housing extremophilic bacteria as a genetic source of relevant thermozymes, promising great potential for innovation in the biotechnology industry.

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Current status and applications of genus Geobacillus in the production of industrially important products—a review

Cited by 20 publications

References 111 publications

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Isolation of Thermophilic Bacteria from Extreme Environments in Northern Chile

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