Current state-of-the-art in plant-based antibody production systems

Donini, Marcello; Marusic, Carla

doi:10.1007/s10529-019-02651-z

Cited by 72 publications

(60 citation statements)

References 72 publications

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“…available on the market, or in pre-clinical and clinical trials (Donini and Marusic 2019). A significant breakthrough was made in 2012, when the US Food and Drug Administration (FDA) approved for the first time a plant cell-derived enzyme, ELELYSO™ (taliglucerase alfa), for human use as a therapeutic for Gaucher disease (Yao et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

et al. 2020

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Key message This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Abstract Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

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Section: Discussionmentioning

confidence: 99%

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

et al. 2020

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“…Another reason for using the model plants is the availability of genome engineered plants to support post-translational modification compatible with the mammalian expressed proteins [75]. Use of non-conventional plant groups such as algae and bryophytes, which were shown to be good protein expression platforms [58] can be an option for scFv expression.…”

Section: Transformation Methodologies Target Plants and Expression Omentioning

confidence: 99%

“…Successful expression of antibodies, biologically and functionally similar to mammalian products is reported in few plants [16,[55][56][57]. Recently, Donini and Marusic reviewed extensively the plant-based antibody production platforms including bryophytes and algal species [58]. A comprehensive list of scFvs expressed in plants has been given in Table 1.…”

Section: Advantages Of Antibody Fragments and Biopharmingmentioning

confidence: 99%

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Satheeshkumar

2020

Mol Biotechnol

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Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker. They offer some advantages over the parental mAb such as low molecular weight, heterologous production, multimeric form, and multivalency. The scFvs were produced against more than 50 antigens till date using 10 different plant species as the expression system. There were considerable improvements in the expression and purification strategies of scFv in the last 24 years. With the growing demand of scFv in therapeutic and diagnostic fields, its biosynthesis needs to be increased. The easiness in development, maintenance, and multiplication of transgenic plants make them an attractive expression platform for scFv production. The review intends to provide comprehensive information about the use of plant expression system to produce scFv. The developments, advantages, pitfalls, and possible prospects of improvement for the exploitation of plants in the industrial level are discussed.

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“…However, the protein yields were low and did not qualify these plants for an industrial platform (Miguel et al, 2019). Recent advances in mAbs production using plant-based systems such as transgenic plants, tissue and cell cultures, and transient expression systems were described recently (Donini and Marusic, 2019). The current status of recombinant biopharmaceutical proteins generated using plant-based systems was well-documented elsewhere (Owczarek et al, 2019).…”

Section: Transgenic Plantsmentioning

confidence: 99%

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi

Shrivastava

2019

Front. Bioeng. Biotechnol.

361

243

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Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.

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Current state-of-the-art in plant-based antibody production systems

Cited by 72 publications

References 72 publications

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

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