Current research developments of patient‑derived tumour xenograft models (Review)

Yin, Zhenyu; Maswikiti, Ewetse Paul; Liu, Qian; Bai, Yuping; Li, Xiaomei; Qi, Wenbo; Liu, Le; Ma, Yanling; Chen, Hao

doi:10.3892/etm.2021.10640

Cited by 6 publications

(4 citation statements)

References 45 publications

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“…Further, several studies have shown high consistency between pre-clinical experiments using PDX models and results of clinical treatment. Therefore, the PDX models are useful in studying tumor pathogenesis and developing personalized treatment [ 16 , 17 , 33 ]. The tumor model generated by transplanting tumor cell lines into mice is different from the primary tumor.…”

Section: Discussionmentioning

confidence: 99%

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Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Xin,

Jiang,

Liu

et al. 2023

Aging

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Background: Head and neck squamous cell carcinomas are the sixth most common malignant tumors worldwide. Tongue squamous cell carcinoma is a common malignant tumor of this type, and it is associated with poor prognosis, a high rate of recurrence and a low survival rate. Plumbagin is derived from Plumbago zeylanica L, several studies report that plumbagin could inhibit cell, tumor metastasis, induce apoptosis in various cancer cells. Patient-derived xenograft (PDX) model can maintain the heterogeneity and microenvironment of human tumors, is a powerful research tool for developing potentially effective therapies for TSCC. Methods: Tumor tissues obtained from TSCC patients were implanted into immunodeficient mice to establish TSCC PDX models. Subsequently, the PDX models were used to evaluate the anti-tumor effects of plumbagin on TSCC. Furthermore, we conducted next-generation sequencing (NGS) and explored the mRNA expression profiles between the treatment and control groups. We selected eight mRNAs related to the characteristics and prognosis of TSCC patients for further analysis. Results: Plumbagin could inhibit the growth of TSCC PDX models and inhibit expression of Akt/mTOR pathway. In addition, plumbagin was shown to increase drug sensitivity to cisplatin. The eight mRNAs selected for further analysis, AXL, SCG5, VOPP1, DCBLD2 and DRAM1 are cancer-promoting genes, DUSP1, AQP5 and BLNK are cancer suppressor genes. And they were related to the diagnosis, growth, prognosis, and immune cell infiltration in TSCC patients. Conclusion: Plumbagin exhibits an inhibitory effect on the growth of the PDX model of TSCC. Moreover, plumbagin enhances the inhibitory effects of cisplatin.

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Section: Discussionmentioning

confidence: 99%

“…The tumor model generated by transplanting tumor cell lines into mice is different from the primary tumor. Therefore, the drug dosage range tends to be different [ 33 ]. Therefore, the PDX model is a more reliable model for evaluating the anti-cancer effects of chemotherapeutic drugs [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Xin,

Jiang,

Liu

et al. 2023

Aging

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“…The uses for these things are varied. Improved diagnosis and treatment of digestive illnesses may be achieved via research into the link between nutrient-rich foods, gut microbiota, and the organisms presently serving as hosts [229][230][231][232]. Patient-derived models are now widely available, allowing their usage by researchers from all across the world.…”

Section: Future Concepts and Opinions Examinedmentioning

confidence: 99%

Simulate the Dynamic Interactions Across Nutritional Supplements, the Polybiome, and the Biochemical Approach Dependent on Patient-Derived Gastrointestinal Knowledge

Reyed¹

2023

J Clin Epid Toxic

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“…In the first series, lymphoma cell implantation was performed via the s.c. route derived from seven patients [40]. After s.c. implantation, two out of three SCNSL samples engrafted at the site of implantation but only one out of four PCNSL biopsies engrafted.…”

Section: Establishment Of Primary and Secondary Cns Lymphoma Pdx Modelsmentioning

confidence: 99%

Patient‐derived xenograft mouse models to investigate tropism to the central nervous system and retina of primary and secondary central nervous system lymphoma

Isbell

Tschuch

Doostkam

et al. 2023

Neuropathology Appl Neurobio

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Aims How and why lymphoma cells home to the central nervous system and vitreoretinal compartment in primary diffuse large B‐cell lymphoma of the central nervous system remain unknown. Our aim was to create an in vivo model to study lymphoma cell tropism to the central nervous system. Methods We established a patient‐derived central nervous system lymphoma xenograft mouse model and characterised xenografts derived from four primary and four secondary central nervous system lymphoma patients using immunohistochemistry, flow cytometry and nucleic acid sequencing technology. In reimplantation experiments, we analysed dissemination patterns of orthotopic and heterotopic xenografts and performed RNA sequencing of different involved organs to detect differences at the transcriptome level. Results We found that xenografted primary central nervous system lymphoma cells home to the central nervous system and eye after intrasplenic transplantation, mimicking central nervous system and primary vitreoretinal lymphoma pathology, respectively. Transcriptomic analysis revealed distinct signatures for lymphoma cells in the brain in comparison to the spleen as well as a small overlap of commonly regulated genes in both primary and secondary central nervous system lymphoma. Conclusion This in vivo tumour model preserves key features of primary and secondary central nervous system lymphoma and can be used to explore critical pathways for the central nervous system and retinal tropism with the goal to find new targets for novel therapeutic approaches.

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Current research developments of patient‑derived tumour xenograft models (Review)

Cited by 6 publications

References 45 publications

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Simulate the Dynamic Interactions Across Nutritional Supplements, the Polybiome, and the Biochemical Approach Dependent on Patient-Derived Gastrointestinal Knowledge

Patient‐derived xenograft mouse models to investigate tropism to the central nervous system and retina of primary and secondary central nervous system lymphoma

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