Current progress in the detection of adrenergic receptor agonist residues in animal-derived foods

Luo, Lei; Pan, Yantong; Li, Qing; Zhang, Yingjie; Chen, Chaochao; Shen, Jianzhong; Wang, Zhanhui

doi:10.1016/j.trac.2022.116875

Cited by 5 publications

(4 citation statements)

References 132 publications

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“… Methods for detecting β-adrenoceptor agonists [ 16 ] (reproduced with permission from Elsevier Ltd.). Reprinted/adapted with permission from Ref.…”

Section: Figurementioning

confidence: 99%

“…SERS-IA is a novel labeling technique in which SERS-enhanced signals are utilized as a readout strategy. These methods are typically developed by capturing the analyte and subsequently modifying the immune substrate, followed by quantification of its concentration through the SERS immunoprobe [ 16 ]. In particular, the high sensitivity and spectral resolution of SERS are crucial for immunoassays.…”

Section: Introductionmentioning

confidence: 99%

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Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists

Wang,

Jing,

Cao

et al. 2024

Foods

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Rapid, sensitive, and accurate detection of adrenoceptor agonists is a significant research topic in the fields of food safety and public health. Immunoassays are among the most widely used methods for detecting adrenoceptor agonists. In recent years, surface-enhanced Raman spectroscopy combined with immunoassay (SERS-IA) has become an effective technique for improving detection sensitivity. This review focuses on the innovation of Raman reporter molecules and substrate materials for the SERS-IA of adrenoceptor agonists. In addition, it also investigates the challenges involved in potentially applying SERS-IA in the detection of adrenoceptor agonists. Overall, this review provides insight into the design and application of SERS-IA for the detection of adrenoceptor agonists, which is critical for animal-derived food safety and public health.

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“… Methods for detecting β-adrenoceptor agonists [ 16 ] (reproduced with permission from Elsevier Ltd.). Reprinted/adapted with permission from Ref.…”

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists

Wang,

Jing,

Cao

et al. 2024

Foods

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“…4,5 As a result, a sandwich immunoassay is mostly used for the detection of macromolecules, such as proteins, while small molecules are overwhelmingly detected by competitive immunoassay. 6 Competitive immunoassays for small molecules frequently exhibit difficulty in meeting the needs of high sensitivity and specificity because of the ceiling affinity of the used antibody. 7,8 Therefore, some pseudosandwich immunoassays, such as those based on antibody variable region fragments, 9−12 antimetatype antibodies, 13,14 anti-idiotypic antibodies, 15,16 and special isolation procedures, 17,18 have been proposed.…”

Section: ■ Introductionmentioning

confidence: 99%

“…A sandwich immunoassay requires analytes to have at least two epitopes that can bind to at least two antibodies simultaneously. However, because of steric hindrance derived from limited size, small molecules are usually considered to be monovalent, such as antibiotics, pesticides, and mycotoxins, which cannot bind to two antibodies at the same time. , As a result, a sandwich immunoassay is mostly used for the detection of macromolecules, such as proteins, while small molecules are overwhelmingly detected by competitive immunoassay …”

Section: Introductionmentioning

confidence: 99%

A Proof-of-Concept Sandwich Enzyme-Linked Immunoassay Development for Small Molecules

Fei,

Jiang,

Bai

et al. 2023

Anal. Chem.

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A sandwich immunoassay theoretically exhibits higher sensitivity and specificity compared to a competitive counterpart; however, it is extremely difficult to obtain a pair of antibodies that can bind to a small molecule simultaneously, which is always thought to be a single epitope. In the present study, abamectin (ABM) was selected to prove the effect of hapten design and antibody recognition properties on the development of a sandwich immunoassay for small molecules. First, the epitopes of ABM were roughly located, and epitope distances were determined. Then, two haptens were designed by introducing spacer arms at the C4″–OH and C5–OH of ABM, respectively, aiming to provide the longest epitope distances. A total of seven rabbit polyclonal antibodies (pAbs) and 21 mouse monoclonal antibodies (mAbs) with various recognition properties were obtained. Extensive combinatorial associations of antibody pairs for simultaneously binding to ABM were performed, and only two mAb–mAb pairs were observed to achieve a sandwich immunoassay for ABM with a total success rate of 0.27%. The best mAb pair for sandwich immunoassay was confirmed by surface plasmon resonance, used to develop a sandwich immunoassay, and then evaluated by cross-reactivities and molecular docking with structurally similar analogues and abamectin. Altogether, the study provided a theoretical foundation as well as practical experience and demonstrated the importance of careful hapten design and extensive antibody screening to successfully establish the sandwich immunoassay for small molecules.

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Hydroxylated hierarchical flower-like COF for solid-phase extraction of adrenergic receptor agonists in milk

Xu,

Liu,

Yang

et al. 2024

Microchim Acta

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Current progress in the detection of adrenergic receptor agonist residues in animal-derived foods

Cited by 5 publications

References 132 publications

Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists

Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists

A Proof-of-Concept Sandwich Enzyme-Linked Immunoassay Development for Small Molecules

Hydroxylated hierarchical flower-like COF for solid-phase extraction of adrenergic receptor agonists in milk

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