Current progress in NK cell biology and NK cell-based cancer immunotherapy

Tarazona, Raquel; López-Sejas, Nelson; Guerrero, Beatriz Delgado; Hassouneh, Fakhri; Valhondo, Isabel; Pera, Alejandra; Sánchez-Correa, Beatriz; Pastor, Nieves; Durán, Esther; Alonso, Corona; Solana, Rafael

doi:10.1007/s00262-020-02532-9

Cited by 37 publications

(33 citation statements)

References 174 publications

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“…S7e). Recent advances suggested that NK cells could also become exhibited after chronic activation, characterized by the upregulation of KIR, NKG2A, LAG3, and HAVCR2 43,44 , but we did not observe the up-regulation of these exhaustion markers in NK cells. Furthermore, NK cells differentially expressed chemokineencoding genes XCL1 and XCL2, which were previously reported to recruit CCR5 + DCs.…”

Section: Resultscontrasting

confidence: 94%

Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma

et al. 2021

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The tumor microenvironment (TME) of nasopharyngeal carcinoma (NPC) harbors a heterogeneous and dynamic stromal population. A comprehensive understanding of this tumor-specific ecosystem is necessary to enhance cancer diagnosis, therapeutics, and prognosis. However, recent advances based on bulk RNA sequencing remain insufficient to construct an in-depth landscape of infiltrating stromal cells in NPC. Here we apply single-cell RNA sequencing to 66,627 cells from 14 patients, integrated with clonotype identification on T and B cells. We identify and characterize five major stromal clusters and 36 distinct subpopulations based on genetic profiling. By comparing with the infiltrating cells in the non-malignant microenvironment, we report highly representative features in the TME, including phenotypic abundance, genetic alternations, immune dynamics, clonal expansion, developmental trajectory, and molecular interactions that profoundly influence patient prognosis and therapeutic outcome. The key findings are further independently validated in two single-cell RNA sequencing cohorts and two bulk RNA-sequencing cohorts. In the present study, we reveal the correlation between NPC-specific characteristics and progression-free survival. Together, these data facilitate the understanding of the stromal landscape and immune dynamics in NPC patients and provides deeper insights into the development of prognostic biomarkers and therapeutic targets in the TME.

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Section: Resultscontrasting

confidence: 94%

Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma

et al. 2021

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“…The potential benefits of NK cell-based therapies over T-cell-based therapies include fewer graft-versus-host reactions and the possibility to develop off-the-shelf products [ 86 , 87 ]. A variety of NK cell products (e.g., autologous NK cells, haploidentical NK cells, chimeric antigen receptor NK cells, stem cell-derived NK cells and NK cell lines) have been explored as NK cell-based therapies [ 86 , 87 ]. There is also an increasing number of methods for enhancing NK activity in vivo as reviewed elsewhere [ 86 , 87 ].…”

Section: Strategies That Exploit Nk Cell Response To Improve Ov Efficaciesmentioning

confidence: 99%

Strategies to Optimise Oncolytic Viral Therapies: The Role of Natural Killer Cells

Leung

McNeish

2021

Viruses

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Oncolytic viruses (OVs) are an emerging class of anti-cancer agents that replicate selectively within malignant cells and generate potent immune responses. Their potential efficacy has been shown in clinical trials, with talimogene laherparepvec (T-VEC or IMLYGIC®) now approved both in the United States and Europe. In healthy individuals, NK cells provide effective surveillance against cancer and viral infections. In oncolytic viral therapy, NK cells may render OV ineffective by rapid elimination of the propagating virus but could also improve therapeutic efficacy by preferential killing of OV-infected malignant cells. Existing evidence suggests that the overall effect of NK cells against OV is context dependent. In the past decade, the understanding of cancer and OV biology has improved significantly, which helped refine this class of treatments in early-phase clinical trials. In this review, we summarised different strategies that have been evaluated to modulate NK activities for improving OV therapeutic benefits. Further development of OVs will require a systematic approach to overcome the challenges of the production and delivery of complex gene and cell-based therapies in clinical settings.

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“…But these three kinds of cells are considered to be the effector cells of the human body's specific immunity, and are the main functional cells for monitoring malignant diseases in the body. NK cells account for about 10% to 15% of peripheral blood lymphocytes [2]. Circulating NK cells in the blood have a shorter survival time, with a half-life of only 7 -10 days [3].…”

Section: Introductionmentioning

confidence: 99%

Clinical Detection of Peripheral Blood Natural Killer Cell Activity

Wang¹,

Li²,

Huang³

et al. 2021

JBM

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Natural Killer (NK) cells are specific immune cells in human immune system. They have a quick effect and can exert a cytotoxic function without prior sensitization, and they show great application potential in cell-based immunotherapy, anti-infection in vivo. NK cell activity in peripheral blood can be used as one of the biomarkers of immune function response. It has a great positive guiding significance for the clinical prognosis of tumor patients, the prevention of cancer and anti-aging. The clinical detection strategies of NK cell activity in circulation mainly grouped into five types: methyl thiazolyl tetrazolium colorimetric, lactate dehydrogenase release, radionuclide labeling, flow cytometry and NK Vue cytokine release method. It has played an important role in different stages of clinical application development. This paper will make a comparative review of the above-mentioned detection strategies for the NK cell activity.

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Current progress in NK cell biology and NK cell-based cancer immunotherapy

Cited by 37 publications

References 174 publications

Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma

Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma

Strategies to Optimise Oncolytic Viral Therapies: The Role of Natural Killer Cells

Clinical Detection of Peripheral Blood Natural Killer Cell Activity

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