Current progress in biosensors for organophosphorus pesticides based on enzyme functionalized nanostructures: a review

Xiong, Sheng; Deng, Yaocheng; Zhou, Yaoyu; Gong, Daoxin; Xu, Zheng; Yang, Lihua; Chen, Henghui; Chen, Ling; Song, Tianwei; Luo, Ao; Deng, Xiaolan; Zhang, Chenyu; Jiang, Zi-yan

doi:10.1039/c8ay01851k

Cited by 62 publications

(24 citation statements)

References 87 publications

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“…In these studies they were able to show a detection limit of 3 nM for the paraoxon substrate. The benefits of combining nanostructures and enzymes in OP sensors has been realized and exploited by several other research groups whose efforts are summarized in a recent review by Sheng et al (Xiong et al, 2018).…”

Section: Relevance and Potential For Deploymentmentioning

confidence: 99%

Enzymatic Bioremediation of Organophosphate Compounds—Progress and Remaining Challenges

Thakur

Medintz

Walper

2019

Front. Bioeng. Biotechnol.

102

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Organophosphate compounds are ubiquitously employed as agricultural pesticides and maintained as chemical warfare agents by several nations. These compounds are highly toxic, show environmental persistence and accumulation, and contribute to numerous cases of poisoning and death each year. While their use as weapons of mass destruction is rare, these never fully disappear into obscurity as they continue to be tools of fear and control by governments and terrorist organizations. Beyond weaponization, their wide-scale dissemination as agricultural products has led to environmental accumulation and intoxication of soil and water across the globe. Therefore, there is a dire need for rapid and safe agents for environmental bioremediation, personal decontamination, and as therapeutic detoxicants. Organophosphate hydrolyzing enzymes are emerging as appealing targets to satisfy decontamination needs owing to their ability to hydrolyze both pesticides and nerve agents using biologically-derived materials safe for both the environment and the individual. As the release of genetically modified organisms is not widely accepted practice, researchers are exploring alternative strategies of organophosphate bioremediation that focus on cell-free enzyme systems. In this review, we first discuss several of the more prevalent organophosphorus hydrolyzing enzymes along with research and engineering efforts that have led to an enhancement in their activity, substrate tolerance, and stability. In the later half we focus on advances achieved through research focusing on enhancing the catalytic activity and stability of phosphotriesterase, a model organophosphate hydrolase, using various approaches such as nanoparticle display, DNA scaffolding, and outer membrane vesicle encapsulation.

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Section: Relevance and Potential For Deploymentmentioning

confidence: 99%

Enzymatic Bioremediation of Organophosphate Compounds—Progress and Remaining Challenges

Thakur

Medintz

Walper

2019

Front. Bioeng. Biotechnol.

102

View full text Add to dashboard Cite

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“…Some of these substrates are best suited for fabricating novel biosensors for OP detection and are discussed elsewhere. 2,96 The immobilization strategies described in this article hold promise as therapeutics and as agents for environmental decontamination. Each strategy has its special use, and the choice of specific immobilization method is influenced by the overall cost, scalability, and applicability.…”

Section: Stabilization/immobilization Strategiesmentioning

confidence: 99%

Enhancing organophosphate hydrolase efficacy via protein engineering and immobilization strategies

Katyal

Chu

Montclare

2020

Annals of the New York Academy of Sciences

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Organophosphorus compounds (OPs), developed as pesticides and chemical warfare agents, are extremely toxic chemicals that pose a public health risk. Of the different detoxification strategies, organophosphate-hydrolyzing enzymes have attracted much attention, providing a potential route for detoxifying those exposed to OPs. Phosphotriesterase (PTE), also known as organophosphate hydrolase (OPH), is one such enzyme that has been extensively studied as a catalytic bioscavenger. In this review, we will discuss the protein engineering of PTE aimed toward improving the activity and stability of the enzyme. In order to make enzyme utilization in OP detoxification more favorable, enzyme immobilization provides an effective means to increase enzyme activity and stability. Here, we present several such strategies that enhance the storage and operational stability of PTE/OPH.

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“…Thus, it is essential to detect PFF in agricultural products. Common detection methods for PFF include gas chromatography (Yang et al, 2012), high-performance liquid chromatography (HPLC) (Raharjo et al, 2009), biosensors Selvolini et al, 2018;Xiong et al, 2018), and colorimetric and fluorescent sensors (Zhang et al, 2014;Li X. et al, 2018;Kovida and Koner, 2020). Although those methods are sensitive, they are time-and reagent-consuming and require a professional operator, and sample preparation is complex.…”

Section: Introductionmentioning

confidence: 99%

Photoresponsive Surface Molecularly Imprinted Polymers for the Detection of Profenofos in Tomato and Mangosteen

Chen

Yang

et al. 2020

Front. Chem.

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As a moderately toxic organophosphorus pesticide, profenofos (PFF) is widely used in agricultural practice, resulting in the accumulation of a high amount of PFF in agricultural products and the environment. This will inevitably damage our health. Therefore, it is important to establish a convenient and sensitive method for the detection of PFF. This paper reports a photoresponsive surface-imprinted polymer based on poly(styrene-co-methyl acrylic acid) (PS-co-PMAA@PSMIPs) for the detection of PFF by using carboxyl-capped polystyrene microspheres (PS-co-PMAA), PFF, 4-((4-(methacryloyloxy)phenyl)diazenyl) benzoic acid, and triethanolamine trimethacrylate as the substrate, template, functional monomer, and cross-linker, respectively. PS-co-PMAA@PSMIP shows good photoresponsive properties in DMSO/H 2 O (3:1, v/v). Its photoisomerization rate constant exhibits a good linear relationship with PFF concentration in the range of 0∼15 µmol/L. PS-co-PMAA@PSMIP was applied for the determination of PFF in spiked tomato and mangosteen with good recoveries ranging in 94.4-102.4%.

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Current progress in biosensors for organophosphorus pesticides based on enzyme functionalized nanostructures: a review

Abstract: Organophosphorus pesticides analysis has become an increasingly significant research area due to their widespread application and contamination of the environment.

Cited by 62 publications

References 87 publications

Enzymatic Bioremediation of Organophosphate Compounds—Progress and Remaining Challenges

Enzymatic Bioremediation of Organophosphate Compounds—Progress and Remaining Challenges

Enhancing organophosphate hydrolase efficacy via protein engineering and immobilization strategies

Photoresponsive Surface Molecularly Imprinted Polymers for the Detection of Profenofos in Tomato and Mangosteen

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