Current Production Capability of Drug-Resistant Pathogen Enables Its Rapid Label-Free Detection Applicable to Wastewater-Based Epidemiology

Miran, Waheed; Long, Xizi; Huang, Wenyuan; Okamoto, Akimitsu

doi:10.3390/microorganisms10020472

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…The defined medium (DM), used as an electrolyte for electrochemical measurements as used in other electrogenic bacteria [ 19 , 20 ], had the following composition (L –1 ): NH 4 Cl: 1 g; MgCl 2 ·6H 2 O: 0.2 g; CaCl 2 ·2H 2 O: 0.08 g; NaHCO 3 : 2.5 g; NaCl: 10 g; and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid: 7.2 g (pH 8.1). Glucose: 10 g was used as an electron donor for electrochemical measurements of the DM (DMG).…”

Section: Methodsmentioning

confidence: 99%

Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate

et al. 2022

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Ultramicrobacteria (UMB) that can pass through a 0.22 µm filter are attractive because of their novelty and diversity. However, isolating UMB has been difficult because of their symbiotic or parasitic lifestyles in the environment. Some UMB have extracellular electron transfer (EET)-related genes, suggesting that these symbionts can grow on an electrode surface independently. Here, we attempted to culture from soil samples bacteria that passed through a 0.22 µm filter poised with +0.2 V vs. Ag/AgCl and isolated Cellulomonas sp. strain NTE-D12 from the electrochemical reactor. A phylogenetic analysis of the 16S rRNA showed 97.9% similarity to the closest related species, Cellulomonas algicola, indicating that the strain NTE-D12 is a novel species. Electrochemical and genomic analyses showed that the strain NTE-D12 generated the highest current density compared to that in the three related species, indicating the presence of a unique electron transfer system in the strain. Therefore, the present study provides a new isolation scheme for cultivating and isolating novel UMB potentially with a symbiotic relationship associated with interspecies electron transfer.

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Section: Methodsmentioning

confidence: 99%

Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate

et al. 2022

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Removal of Ampicillin Using Cold Atmospheric-Pressure Plasma Jet and Its Plasma-Activated Water

et al. 2023

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Ampicillin (AMP) is one broad-spectrum β-lactam antibiotics applied widely in the world, and its abuse may cause microbial resistance and pose a threat to human health. Currently, there is still a lack of effective methods for treating various antibiotics in the environment. In the present work, we employed cold atmospheric-pressure plasma (CAP) to treat ampicillin and explored the optimal conditions for the removal of AMP in water, and we made use of CAP jets (CAPJ) with different working gases and also took advantage of the associated plasma-activated water (PAW) to improve the treatment efficiency. Our results showed that air-CAPJ combined with its PAW treatment could best enhance the efficiency of the removal of ampicillin in water. For the mechanism of the AMP degradation by CAPJ and PAW treatment, hydroxyl radical (·OH) was the key factor in the direct plasma processing, while singlet oxygen (<sup>1</sup>O<sub>2</sub>) played the critical role in the indirect PAW treatment. The toxicological assessment confirmed the bio-safety of the CAPJ treatments. Therefore, this work demonstrates an effective method for removing antibiotics such as ampicillin in the environment.

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Living and Regenerative Material Encapsulating Self-Assembled Shewanella oneidensis-CdS Hybrids for Photocatalytic Biodegradation of Organic Dyes

Tao

Jin

et al. 2022

Microorganisms

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Reductive biodegradation by microorganisms has been widely explored for detoxifying recalcitrant contaminants; however, the biodegradation capacity of microbes is limited by the energy level of the released electrons. Here, we developed a method to self-assemble Shewanella oneidensis-CdS nanoparticle hybrids with significantly improved reductive biodegradation capacity and constructed a living material by encapsulating the hybrids in hydrogels. The material confines the nano-bacteria hybrids and protects them from environmental stress, thus improving their recyclability and long-term stability (degradation capacity unhindered after 4 weeks). The developed living materials exhibited efficient photocatalytic biodegradation of various organic dyes including azo and nitroso dyes. This study highlights the feasibility and benefits of constructing self-assembled nano-bacteria hybrids for bioremediation and sets the stage for the development of novel living materials from nano-bacteria hybrids.

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Current Production Capability of Drug-Resistant Pathogen Enables Its Rapid Label-Free Detection Applicable to Wastewater-Based Epidemiology

Cited by 3 publications

References 21 publications

Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate

Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate

Removal of Ampicillin Using Cold Atmospheric-Pressure Plasma Jet and Its Plasma-Activated Water

Living and Regenerative Material Encapsulating Self-Assembled Shewanella oneidensis-CdS Hybrids for Photocatalytic Biodegradation of Organic Dyes

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