Current Methods in the Study of Nanomaterials for Bone Regeneration

Tanaka, Manabu; Izumiya, Makoto; Haniu, Hisao; Ueda, Katsumi; Ma, Chuang; Ueshiba, Koki; Ideta, Hirokazu; Sobajima, Atsushi; Uchiyama, Shigeharu; Takahashi, J.; Saito, Naoto

doi:10.3390/nano12071195

Cited by 9 publications

(6 citation statements)

References 104 publications

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“…Osteoblasts derived from multipotent MSCs are critical players in bone formation and repair [30]. During bone healing, osteoblasts can proliferate and deposit bone ECM components in the injury sites [31].…”

Section: Discussionmentioning

confidence: 99%

The role of proteoglycan form of DMP1 in cranial repair

Liu

Niu

Zhou

et al. 2022

BMC Mol and Cell Biol

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Background The cranial region is a complex set of blood vessels, cartilage, nerves and soft tissues. The reconstruction of cranial defects caused by trauma, congenital defects and surgical procedures presents clinical challenges. Our previous data showed that deficiency of the proteoglycan (PG) form of dentin matrix protein 1 (DMP1-PG) could lead to abnormal cranial development. In addition, DMP1-PG was highly expressed in the cranial defect areas. The present study aimed to investigate the potential role of DMP1-PG in intramembranous ossification in cranial defect repair. Methods Mouse cranial defect models were established by using wild- type (WT) and DMP1-PG point mutation mice. Microcomputed tomography (micro-CT) and histological staining were performed to assess the extent of repair. Immunofluorescence assays and real-time quantitative polymerase chain reaction (RT‒qPCR) were applied to detect the differentially expressed osteogenic markers. RNA sequencing was performed to probe the molecular mechanism of DMP1-PG in regulating defect healing. Results A delayed healing process and an abnormal osteogenic capacity of primary osteoblasts were observed in DMP1-PG point mutation mice. Furthermore, impaired inflammatory signaling pathways were detected by using RNA transcription analysis of this model. Conclusions Our data indicate that DMP1-PG is an indispensable positive regulator during cranial defect healing.

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Section: Discussionmentioning

confidence: 99%

The role of proteoglycan form of DMP1 in cranial repair

Liu

Niu

Zhou

et al. 2022

BMC Mol and Cell Biol

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“…The presence of bio-corona on the surface of carbon-based nanoparticles may alter their activity, biodistribution, pharmacokinetics, cellular uptake, toxicity, and clearance. [28,29] In BTE, NPs can be incorporated into bone scaffolds to act as fillers and provide mechanical support, or they can be employed as carriers for delivering bioactive molecules that stimulate bone regeneration [30]. Recently, special attention has been directed toward polymeric nanoparticles (PNPs) due to their many features, such as biocompatibility, biodegradability, water solubility, and lack of immunogenicity [31].…”

Section: Nanoparticles Classificationsmentioning

confidence: 99%

Harnessing the Potential of PLGA Nanoparticles for Enhanced Bone Regeneration

Hassan,

Abdelnabi,

Mohsin

2024

Pharmaceutics

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Recently, nanotechnologies have become increasingly prominent in the field of bone tissue engineering (BTE), offering substantial potential to advance the field forward. These advancements manifest in two primary ways: the localized application of nanoengineered materials to enhance bone regeneration and their use as nanovehicles for delivering bioactive compounds. Despite significant progress in the development of bone substitutes over the past few decades, it is worth noting that the quest to identify the optimal biomaterial for bone regeneration remains a subject of intense debate. Ever since its initial discovery, poly(lactic-co-glycolic acid) (PLGA) has found widespread use in BTE due to its favorable biocompatibility and customizable biodegradability. This review provides an overview of contemporary advancements in the development of bone regeneration materials using PLGA polymers. The review covers some of the properties of PLGA, with a special focus on modifications of these properties towards bone regeneration. Furthermore, we delve into the techniques for synthesizing PLGA nanoparticles (NPs), the diverse forms in which these NPs can be fabricated, and the bioactive molecules that exhibit therapeutic potential for promoting bone regeneration. Additionally, we addressed some of the current concerns regarding the safety of PLGA NPs and PLGA-based products available on the market. Finally, we briefly discussed some of the current challenges and proposed some strategies to functionally enhance the fabrication of PLGA NPs towards BTE. We envisage that the utilization of PLGA NP holds significant potential as a potent tool in advancing therapies for intractable bone diseases.

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“…The murine calvaria-derived osteoblast-like cell line (MC3T3-E1) was procured from Riken BRC (Tsukuba, Ibaraki, Japan). The MC3T3-E1 cells were cultured in alpha-minimum essential medium (αMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 10% FBS and 1% penicillin-streptomycin (Fujifilm Wako) [24,25]. The passaging of cells was performed every three days, and the cells were cultured at 37 • C under 5% CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

Three-Dimensional Modeling with Osteoblast-like Cells under External Magnetic Field Conditions Using Magnetic Nano-Ferrite Particles for the Development of Cell-Derived Artificial Bone

Ma,

Izumiya,

Nobuoka

et al. 2024

Nanomaterials

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The progress in artificial bone research is crucial for addressing fractures and bone defects in the aging population. However, challenges persist in terms of biocompatibility and structural complexity. Nanotechnology provides a promising avenue by which to overcome these challenges, with nano-ferrite particles (NFPs) exhibiting superparamagnetic properties. The ability to control cell positioning using a magnetic field opens up new possibilities for customizing artificial bones with specific shapes. This study explores the biological effects of NFPs on osteoblast-like cell lines (MC3T3-E1), including key analyses, such as cell viability, cellular uptake of NFPs, calcification processes, cell migration under external magnetic field conditions, and three-dimensional modeling. The results indicate that the impact of NFPs on cell proliferation is negligible. Fluorescence and transmission electron microscopy validated the cellular uptake of NFPs, demonstrating the potential for precise cell positioning through an external magnetic field. Under calcification-inducing conditions, the cells exhibited sustained calcification ability even in the presence of NFPs. The cell movement analysis observed the controlled movement of NFP-absorbing cells under an external magnetic field. Applying a magnetic field along the z-axis induced the three-dimensional shaping of cells incorporating NFPs, resulting in well-arranged z-axis directional patterns. In this study, NFPs demonstrated excellent biocompatibility and controllability under an external magnetic field, laying the foundation for innovative treatment strategies for customizing artificial bones.

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Current Methods in the Study of Nanomaterials for Bone Regeneration

Cited by 9 publications

References 104 publications

The role of proteoglycan form of DMP1 in cranial repair

The role of proteoglycan form of DMP1 in cranial repair

Harnessing the Potential of PLGA Nanoparticles for Enhanced Bone Regeneration

Three-Dimensional Modeling with Osteoblast-like Cells under External Magnetic Field Conditions Using Magnetic Nano-Ferrite Particles for the Development of Cell-Derived Artificial Bone

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