Current methods in quantifying ROS and oxidative damage in Caenorhabditis elegans and other model organism of aging

Labuschagne, Christiaan F.; Brenkman, Arjan B.

doi:10.1016/j.arr.2013.09.003

Cited by 82 publications

(71 citation statements)

References 131 publications

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“…Such ROS-related mechanisms have been described in hydras and other cnidarians where they are involved in growth, development and reproduction (Blackstone, 2003; Habetha and Bosch, 2005); and ROS are involved in injury-induced cell death and activation of regeneration in Hydra, as well as in Drosophila and zebrafish (reviewed in Vriz et al, 2014). In essentially all species that age there occurs increased accumulation of oxidatively damage molecules, and reducing this oxidative damage can extend lifespan in experimental animals such as yeast and C. elegans (for review see Labuschagne and Brenkman, 2013; Merksamer et al, 2013). …”

Section: Mechanisms Of Aging Overviewmentioning

confidence: 99%

Aging and longevity in the simplest animals and the quest for immortality

Petralia

Mattson

Yao

2014

Ageing Research Reviews

113

107

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Here we review the examples of great longevity and potential immortality in the earliest animal types and contrast and compare these to humans and other higher animals. We start by discussing aging in single-celled organisms such as yeast and ciliates, and the idea of the immortal cell clone. Then we describe how these cell clones could become organized into colonies of different cell types that lead to multicellular animal life. We survey aging and longevity in all of the basal metazoan groups including ctenophores (comb jellies), sponges, placozoans, cnidarians (hydras, jellyfish, corals and sea anemones) and myxozoans. Then we move to the simplest bilaterian animals (with a head, three body cell layers, and bilateral symmetry), the two phyla of flatworms. A key determinant of longevity and immortality in most of these simple animals is the large numbers of pluripotent stem cells that underlie the remarkable abilities of these animals to regenerate and rejuvenate themselves. Finally, we discuss briefly the evolution of the higher bilaterians and how longevity was reduced and immortality lost due to attainment of greater body complexity and cell cycle strategies that protect these complex organisms from developing tumors. We also briefly consider how the evolution of multiple aging-related mechanisms/pathwayshinders our ability to understand and modify the aging process in higher organisms.

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Section: Mechanisms Of Aging Overviewmentioning

confidence: 99%

Aging and longevity in the simplest animals and the quest for immortality

Petralia

Mattson

Yao

2014

Ageing Research Reviews

113

107

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“…Despite the fact that ROS is necessary for organisms to perform some important cell processes, excessive ROS can damage cell components including proteins, lipids, and DNA (Preiser 2012;Imlay 2013;Kim and Park 2014). In bacteria, oxidative stress usually modifies certain macromolecules to facilitate their evolution as well as generate a variety of cellular damages (Brierley and Martin 2013;Labuschagne and Brenkman 2013;Schieber and Chandel 2014). In humans, oxidative damages result in diseases and aging (Gupta et al 2014;Lionaki and Tavernarakis 2013;Venkataraman et al 2013).…”

Section: Introductionmentioning

confidence: 98%

Proteomic insights into the functional basis for the response regulator DrRRA ofDeinococcus radiodurans

Wang

Liu

et al. 2016

International Journal of Radiation Biology

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Purpose To investigate the function basis of the recently discovered response regulator, drRRA (DNA damage response regulator A) in Deinococcus radiodurans, we compared the proteomic profile of the radiation-sensitive drRRA mutant with that of wild-type strain under both non-stress and gamma radiation treatment. Materials and methods Total proteins of D. radiodurans cells were subjected to two-dimension electrophoresis. Protein spots in 2-Dimension gels were silver stained and scanned. Spots that changed significantly in expression levels were selected for mass spectrometry analysis. Seven genes encoding representative proteins were knocked out for stress resistance analysis. Results A total of 52 proteins displayed significant expression level changes at least 1.5-fold in the mutant relative to wild-type strain under non-stress conditions, with 31 repressed and 21 induced proteins, which might affect the cell response of D. radiodurans to gamma radiation. The proteins were distributed into functional groups including stress response, metabolism, and function unknown. Disruptions of several altered proteins including DRA0259 (Catalase E) and DR1538 (Osmotically inducible protein C), reduced the antioxidant activity of D. radiodurans. Conclusion Combined with our previous result of transcriptional profile, we further confirmed that inactivation of DrRRA affects the expression of various stress response systems.

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“…This biochemical reaction turns H 2 DCFDA into the non-fluorescent compound H 2 DCF which is then rapidly oxidized to highly fluorescent 2′,7′-dichlorofluorescein (DCF) in the presence of ROS (Figure 1A). Therefore, fluorescence signals from H 2 DCFDA probe demonstrate important information for the quantification of ROS at single cell level (Labuschagne and Brenkman, 2013). The Caenorhabditis elegans model system provides an excellent in vivo experimental environment for evaluating molecular mechanisms of ROS pathophysiology due to their short lifespan, simplicity, and ease of genetic manipulation (Labuschagne and Brenkman, 2013; Miranda-Vizuete and Veal, 2017; Yoon et al , 2017) (Figure 1B).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, fluorescence signals from H 2 DCFDA probe demonstrate important information for the quantification of ROS at single cell level (Labuschagne and Brenkman, 2013). The Caenorhabditis elegans model system provides an excellent in vivo experimental environment for evaluating molecular mechanisms of ROS pathophysiology due to their short lifespan, simplicity, and ease of genetic manipulation (Labuschagne and Brenkman, 2013; Miranda-Vizuete and Veal, 2017; Yoon et al , 2017) (Figure 1B). Here, we describe a simple protocol to measure the levels of time-course ROS generation in C. elegans using H 2 DCFDA under normal and heat- or chemically-induced oxidative stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate

Yoon¹,

Lee²,

Seok³

2018

BIO-PROTOCOL

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Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, cardiovascular disease, ischemia-reperfusion and aging. Over the past several decades, antioxidants have received explosive attention regarding their protective potential against these deleterious reactions. Accordingly, many analytical methodologies have been developed for the evaluation of the antioxidant capacity of compounds or complex biological samples. Herein, we introduce a simple and convenient method to detect in vivo intracellular ROS levels photometrically in Caenorhabditis elegans using 2′,7′-dichlorofluorescein diacetate (H2DCFDA), a cell permeant tracer.

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Current methods in quantifying ROS and oxidative damage in Caenorhabditis elegans and other model organism of aging

Cited by 82 publications

References 131 publications

Aging and longevity in the simplest animals and the quest for immortality

Aging and longevity in the simplest animals and the quest for immortality

Proteomic insights into the functional basis for the response regulator DrRRA ofDeinococcus radiodurans

Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate

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