Current Laboratory Methods for Biological Threat Agent Identification

Henchal, Erik A.; Teska, Jeffrey D.; Ludwig, George V.; Shoemaker, David R.; Ezzell, John W.

doi:10.1016/s0272-2712(18)30027-1

Cited by 41 publications

(30 citation statements)

References 43 publications

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“…⌬Rn, change in fluorescence. (9). In addition, the use of this technology in field laboratories has become a reality now that instruments such as the R.A.P.I.D.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the use of this technology in field laboratories has become a reality now that instruments such as the R.A.P.I.D. and SmartCycler XC instruments, which have been specifically designed for use by field medical laboratories, have become available (9). In order to obtain an early diagnosis and contain future outbreaks of Ebola hemorrhagic fever, it will be necessary to implement rapid diagnostic assays in laboratories throughout Africa.…”

Section: Discussionmentioning

confidence: 99%

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Development and Evaluation of a Fluorogenic 5′ Nuclease Assay To Detect and Differentiate between Ebola Virus Subtypes Zaire and Sudan

et al. 2001

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The ability to rapidly recognize Ebola virus infections is critical to quickly limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of Ebola virus infection and to distinguish it from other diseases that can cause similar clinical symptoms. A one-tube reverse transcription-PCR assay for the identification of Ebola virus subtype Zaire (Ebola Zaire) and Ebola virus subtype Sudan (Ebola Sudan) was developed and evaluated by using the ABI PRISM 7700 sequence detection system. This assay uses one common primer set and two differentially labeled fluorescent probes to simultaneously detect and differentiate these two subtypes of Ebola virus. The sensitivity of the primer set was comparable to that of previously designed primer sets, as determined by limit-of-detection experiments. This assay is unique in its ability to simultaneously detect and differentiate Ebola Zaire and Ebola Sudan. In addition, this assay is compatible with emerging rapid nucleic acid analysis platforms and therefore may prove to be a very useful diagnostic tool for the control and management of future outbreaks.

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“…⌬Rn, change in fluorescence. (9). In addition, the use of this technology in field laboratories has become a reality now that instruments such as the R.A.P.I.D.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Fluorogenic 5′ Nuclease Assay To Detect and Differentiate between Ebola Virus Subtypes Zaire and Sudan

et al. 2001

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“…Several papers also reported highly sensitive bacterial and viral assays involving ECL [166][167][168] as well as cancer antigens [169][170][171][172] in a variety of templates employing immunoassays and nucleic acid amplification techniques. E. coli 0157 studies have been done for water safety and food, such as drinking [173] and creek [174] water, feces [175], various food and environmental water matrices [176][177][178], and ground beef, chicken, fish, juices, and milk [179,180].…”

Section: Food and Water Safety And Military/defense Applicationsmentioning

confidence: 99%

Applications of Electrochemiluminescence

Parveen

Aslam

et al. 2013

SpringerBriefs in Molecular Science

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The development of electrochemiluminescence (ECL) applications is a growing field, having the potential advantages of ECL over conventional chemiluminescence. ECL has found various applications in immunoassays, DNA probe assays, and aptasensors by employing ECL-active species as labels on biological molecules. The recent use of ECL to detect many chemically, biochemically, clinically, and environmentally important analytes is reviewed.

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“…In either situation, knowledge of the type and extent of bacterial contamination requires the use of a fast analytical technique with high sensitivity and selectivity. Traditional methods for pathogen detection require the collection and growth of microorganisms prior to biochemical assays, which is both time consuming and, dependent on growth media, may lead to biased results because of selective cell outgrowth 1,2. In recent years, the polymerase chain reaction (PCR) has increasingly been used to detect bacterial DNA 3,4.…”

Section: Introductionmentioning

confidence: 99%

Affinity Capture Mass Spectrometry of Biomarker Proteins Using Peptide Ligands from Biopanning

et al. 2009

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Affinity capture mass spectrometry was used to isolate and ionize protein A from Staphylococcus aureus from both a commercial source and cell culture lysate using matrix assisted laser desorption/ ionization mass spectrometry. Two surfaces are compared: gold surfaces with immunoglobulin G covalently immobilized and silica surfaces with a covalently bound small peptide discovered via biopanning. A detection limit of 2.22 bacterial cells/mL of culture fluid was determined using the immobilized peptide surfaces. This study emphasizes the ability to use peptide ligands to effectively capture a biomarker protein out of a complex mixture. This demonstrates the potential to use biopanning to generate capture ligands for a large variety of target proteins, and subsequently detect the captured protein using MALDI mass spectrometry.

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Current Laboratory Methods for Biological Threat Agent Identification

Cited by 41 publications

References 43 publications

Development and Evaluation of a Fluorogenic 5′ Nuclease Assay To Detect and Differentiate between Ebola Virus Subtypes Zaire and Sudan

Development and Evaluation of a Fluorogenic 5′ Nuclease Assay To Detect and Differentiate between Ebola Virus Subtypes Zaire and Sudan

Applications of Electrochemiluminescence

Affinity Capture Mass Spectrometry of Biomarker Proteins Using Peptide Ligands from Biopanning

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