Current Laboratory Biosecurity for Handling Pathogenic Viruses

Artika, I Made; Ma’roef, Chairin Nisa

doi:10.3844/ajbbsp.2018.108.116

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…161 The importance of biosecurity systems to prevent loss, theft, misuse, diversion, or intentional unauthorised release of pathogenic viruses handled in the laboratory has previously been described. 163 For PCR detection of pathogenic viruses, the genetic material is first extracted, isolated and purified. EV-A71 RNA extraction is commonly carried out using commercial kits.…”

Section: Molecular Mechanism Of Enterovirus A71 Infection and Innate ...mentioning

confidence: 99%

“…All manipulation of infectious viral specimen material and suspensions must be performed inside a functional class II biological safety cabinet 161 . The importance of biosecurity systems to prevent loss, theft, misuse, diversion, or intentional unauthorised release of pathogenic viruses handled in the laboratory has previously been described 163 …”

Section: Molecular Detection and Characterisation Of Enterovirus A71mentioning

confidence: 99%

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Insight into the Enterovirus A71: A review

Kinobe

Wiyatno

Artika

et al. 2022

Reviews in Medical Virology

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Enterovirus A71 is a major causative pathogen of hand, foot and mouth disease. It has become a global public health threat, and is especially important for infants and young children in the Asian‐Pacific countries. The enterovirus A71 is a non‐enveloped virus of the Picornaviridae family having a single‐stranded positive‐sense RNA genome of about 7.4 kb which encodes the structural and nonstructural proteins. Currently there are no US FDA‐approved vaccines or antiviral therapy available against enterovirus A71 infection. Although enterovirus A71 vaccines have been licenced in China, clinically approved vaccines for widespread vaccination programs are lacking. Substantial progress has recently been achieved on understanding the structure and function of enterovirus A71 proteins together with information on the viral genetic diversity and geographic distribution. The present review is intended to provide an overview on our current understanding of the molecular biology and epidemiology of enterovirus A71 which will aid the development of vaccines, therapeutics and other control strategies so as to bolster the preparedness for future enterovirus A71 outbreaks.

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Section: Molecular Mechanism Of Enterovirus A71 Infection and Innate ...mentioning

confidence: 99%

Section: Molecular Detection and Characterisation Of Enterovirus A71mentioning

confidence: 99%

Insight into the Enterovirus A71: A review

Kinobe

Wiyatno

Artika

et al. 2022

Reviews in Medical Virology

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“…Indeed, with the current advanced powerful genetic engineering techniques, a virus can be modified, redesigned, reconstructed or even synthesized. This can potentially bring about a novel virus which is unprecedented in nature ( Artika and Ma'roef, 2018 ). The molecular characteristics of the SARS-CoV-2 indicated that the virus is not a product of purposeful manipulation.…”

Section: Molecular Characteristics Of Sars-cov-2mentioning

confidence: 99%

Molecular biology of coronaviruses: current knowledge

Artika

Dewantari

Wiyatno

2020

Heliyon

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The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) late December 2019 in Wuhan, China, marked the third introduction of a highly pathogenic coronavirus into the human population in the twenty-first century. The constant spillover of coronaviruses from natural hosts to humans has been linked to human activities and other factors. The seriousness of this infection and the lack of effective, licensed countermeasures clearly underscore the need of more detailed and comprehensive understanding of coronavirus molecular biology. Coronaviruses are large, enveloped viruses with a positive sense single-stranded RNA genome. Currently, coronaviruses are recognized as one of the most rapidly evolving viruses due to their high genomic nucleotide substitution rates and recombination. At the molecular level, the coronaviruses employ complex strategies to successfully accomplish genome expression, virus particle assembly and virion progeny release. As the health threats from coronaviruses are constant and long-term, understanding the molecular biology of coronaviruses and controlling their spread has significant implications for global health and economic stability. This review is intended to provide an overview of our current basic knowledge of the molecular biology of coronaviruses, which is important as basic knowledge for the development of coronavirus countermeasures.

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“…To address the possibility that pathogenic viruses may be misused for malicious purposes, implementation of a biosecurity system in any laboratory working with pathogenic viruses is recommended. The laboratory biosecurity system should cover physical security, virus control and accountability, personnel security, transport security, and information security; the implementation of which needs to be integrated with the overall biosafety plan (Artika and Ma'roef, 2018).…”

Section: Development Of Laboratory Capacitymentioning

confidence: 99%