Current knowledge on the multiform reconstitution of intestinal stem cell niche

Xu, Ziyan; Huang, Jinjian; Liu, Ye; Zhao, Yun; Wu, Xiuwen; Ren, Jianan

doi:10.4252/wjsc.v13.i10.1564

Cited by 2 publications

(2 citation statements)

References 96 publications

(120 reference statements)

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“…Studies concerning in vitro cultures of human primary intestinal tissue described various protocols and different supplements aimed to optimize colonoid growth and expansion [17,25,36]. Nevertheless, the precise conditions for growing such organoids in terms of crypt isolation and culture media are still being tested [37]. Here, we describe the establishment of an ASC human colonic organoid system derived from adenomas and surrounding healthy tissue obtained from various polyposis patients.…”

Section: Discussionmentioning

confidence: 99%

Adenoma-Derived Organoids for Precision Therapy

et al. 2022

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Human colonic organoids derived from adult tissue biopsies are based on the ability of isolated somatic epithelial stem cells to reconstitute the structure and function of the colon, offering new opportunities for studying the biology of the large intestine in both health and disease. These colonoids may also function as efficient platforms for drug screening and discovery. Here, we describe the establishment of human colonic organoids derived from healthy, and adenomatous polyp tissues. We then demonstrate that organoids grown from adenomas of familial adenomatous polyposis (FAP) patients harboring nonsense mutations in the tumor suppressor gene adenomatous polyposis coli (APC), can be used to establish a personalized therapeutic strategy which relies on nonsense mutation readthrough therapy.

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Section: Discussionmentioning

confidence: 99%

Adenoma-Derived Organoids for Precision Therapy

et al. 2022

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“…Organoids are often referred to as mini-organs, and current research is focused on creating fully functional organs on a mini-scale. Examples include ‘mini-guts’ [ 53 ], ‘mini-hearts’ [ 54 ], and ‘mini-livers’ [ 55 ]. The name ‘organoid’ accurately describes a miniaturised model of an entire organ.…”

Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.

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Current knowledge on the multiform reconstitution of intestinal stem cell niche

Cited by 2 publications

References 96 publications

Adenoma-Derived Organoids for Precision Therapy

Adenoma-Derived Organoids for Precision Therapy

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

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